##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      Aor51HI     
ApaLI       AscI        Asp700I     Asp718I     AspEI       
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BsaHI       BsiEI       BsiWI       BsmBI       
BsoBI       BspDI       BspHI       BsrBI       BsrFI       
BssHII      BssSI       BstEII      BstPI       BstUI       
BstXI       Cfr10I      ClaI        CpoI        DraIII      
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRV       
FbaI        FseI        FspI        HgaI        Hin1I       
HindIII     KasI        KpnI        KspI        MfeI        
MluI        MluNI       MscI        MunI        MvnI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NheI        NotI        NruI        PacI        PaeR7I      
PinAI       PmeI        PpuMI       PshAI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SphI        Sse8387I    
StuI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI         2842  GTATAC               GT^[AC][GT]AC
AccIII       1889  TCCGGA               T^CCGGA
AciI         1741  GCGG                 CCGC(-3/-1)
AocI         3189  CCTTAGG              CC^T[ACGT]AGG
ApaI         1792  GGGCCC               GGGCC^C
AspHI        2048  GTGCTC               G[AT]GC[AT]^C
AspI         1117  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII        2142  GGACC                G^G[AT]CC
BanII        1792  GGGCCC               G[GA]GC[CT]^C
BbsI         3416  GTCTTC               GAAGAC(2/6)
BlpI         2112  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2112  GCTGAGC              GC^T[ACGT]AGC
BpuAI        3416  GTCTTC               GAAGAC(2/6)
BsaBI        1213  GATGTATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          796  GAGACC               GGTCTC(1/5)
BseAI        1889  TCCGGA               T^CCGGA
BsiHKAI      2048  GTGCTC               G[AT]GC[AT]^C
BsmI          702  GAATGC               GAATGC(1/-1)
BspEI        1889  TCCGGA               T^CCGGA
Bst1107I     2842  GTATAC               GTA^TAC
BstBI        4120  TTCGAA               TT^CGAA
BstYI         908  AGATCC               [GA]^GATC[CT]
BstZ17I      2842  GTATAC               GTA^TAC
Bsu36I       3189  CCTTAGG              CC^T[ACGT]AGG
CelII        2112  GCTGAGC              GC^T[ACGT]AGC
DraII        1791  AGGGCCC              [GA]G^G[ACGT]CC[CT]
DsaI         1752  CCATGG               C^C[GA][CT]GG
Eco81I       3189  CCTTAGG              CC^T[ACGT]AGG
EcoO109I     1791  AGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoRI         846  GAATTC               G^AATTC
EcoT22I      2727  ATGCAT               ATGCA^T
FauI         1741  GCGGG                CCCGC(4/6)
HaeII         416  AGCGCC               [GA]GCGC^[CT]
HincII       1677  GTTAAC               GT[CT]^[GA]AC
HindII       1677  GTTAAC               GT[CT]^[GA]AC
HpaI         1677  GTTAAC               GTT^AAC
MamI         1213  GATGTATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          908  AGATCC               [GA]^GATC[CT]
MroI         1889  TCCGGA               T^CCGGA
NcoI         1752  CCATGG               C^CATGG
NsiI         2727  ATGCAT               ATGCA^T
NspV         4120  TTCGAA               TT^CGAA
PflFI        1117  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
ScaI         2800  AGTACT               AGT^ACT
SfuI         4120  TTCGAA               TT^CGAA
SnaBI        4052  TACGTA               TAC^GTA
Tth111I      1117  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII         908  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AlwI          909  GATCC                GGATC(4/5)
             3579  GATCC               
BbrPI         568  CACGTG               CAC^GTG
             2053  CACGTG              
BseRI         751  CTCCTC               GAGGAG(10/8)
             3516  CTCCTC              
BsgI          405  GTGCAG               GTGCAG(16/14)
             1208  GTGCAG              
BsmFI        1579  GGGAC                GGGAC(10/14)
             1743  GGGAC               
Bsp1407I       35  TGTACA               T^GTACA
             3027  TGTACA              
BspMI        3726  GCAGGT               ACCTGC(4/8)
             3769  GCAGGT              
BsrGI          35  TGTACA               T^GTACA
             3027  TGTACA              
BstAPI        309  GCAAGTTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1458  GCAGTACATGC         
CfoI          417  GCGC                 GCG^C
             1851  GCGC                
HhaI          417  GCGC                 GCG^C
             1851  GCGC                
HinP1I        417  GCGC                 G^CGC
             1851  GCGC                
MspA1I         43  CAGCTG               C[AC]G^C[GT]G
              835  CAGCTG              
PmaCI         568  CACGTG               CAC^GTG
             2053  CACGTG              
PmlI          568  CACGTG               CAC^GTG
             2053  CACGTG              
PstI          550  CTGCAG               CTGCA^G
              764  CTGCAG              
PvuII          43  CAGCTG               CAG^CTG
              835  CAGCTG              
SpeI          282  ACTAGT               A^CTAGT
             2423  ACTAGT              
SspBI          35  TGTACA               T^GTACA
             3027  TGTACA              
SwaI         2916  ATTTAAAT             ATTT^AAAT
             4189  ATTTAAAT            
XbaI           26  TCTAGA               T^CTAGA
             1353  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AcsI        AfaI        AflIII      AluI        AlwNI       
ApoI        AseI        AsnI        BbvI        BfaI        
BmyI        BpmI        BsaAI       BsaJI       BsaWI       
BsiYI       BslI        BsmAI       Bsp1286I    BspLU11I    
BsrDI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       DraI        
EarI        EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PshBI       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I