##################################
# NO CUT ENZYMES (   82 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflIII      ApoI        AscI        Asp700I     Asp718I     
AvrII       BamHI       BbrPI       BclI        BglII       
BlnI        BsaBI       BseAI       BsiWI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrGI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     ClaI        CpoI        DraI        DrdI        
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        HincII      HindII      HindIII     HpaI        
KpnI        MamI        MfeI        MluI        MroI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
RcaI        RsrII       SalI        SapI        SbfI        
SexAI       SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AatII        1270  GACGTC               GACGT^C
AflII        3969  CTTAAG               C^TTAAG
AgeI         3148  ACCGGT               A^CCGGT
AhdI         1667  GACTCCGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       3385  GTGCAC               G^TGCAC
ApaLI        3385  GTGCAC               G^TGCAC
AseI         3867  ATTAAT               AT^TAAT
AsnI         3867  ATTAAT               AT^TAAT
AspEI        1667  GACTCCGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         797  TGCGCA               TGC^GCA
BbsI         3954  GAAGAC               GAAGAC(2/6)
BcgI          382  CGATTCTTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1870  GTATCC               GTATCC(6/5)
BfrI         3969  CTTAAG               C^TTAAG
BlpI         1369  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1369  GCTCAGC              GC^T[ACGT]AGC
BpuAI        3954  GAAGAC               GAAGAC(2/6)
BsaWI        3148  ACCGGT               [AT]^CCGG[AT]
BsmI         1526  GAATGC               GAATGC(1/-1)
BspMI        1928  ACCTGC               ACCTGC(4/8)
BssSI        1616  CACGAG               CACGAG(-5/-1)
CelII        1369  GCTCAGC              GC^T[ACGT]AGC
DraIII       2690  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     1667  GACTCCGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI          797  TGCGCA               TGC^GCA
NcoI          804  CCATGG               C^CATGG
PflMI         711  CCAAAATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        3148  ACCGGT               A^CCGGT
PshBI        3867  ATTAAT               AT^TAAT
SacI         1289  GAGCTC               GAGCT^C
ScaI         2600  AGTACT               AGT^ACT
Van91I        711  CCAAAATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AocI          197  CCTGAGG              CC^T[ACGT]AGG
             2891  CCTCAGG             
Aor51HI       730  AGCGCT               AGC^GCT
             1721  AGCGCT              
AspI         3465  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3768  GACTCCGTC           
BbeI          188  GGCGCC               GGCGC^C
             2847  GGCGCC              
BsrDI        2340  GCAATG               GCAATG(2/0)
             2558  GCAATG              
BstYI        2461  AGATCC               [GA]^GATC[CT]
             3877  GGATCT              
Bsu36I        197  CCTGAGG              CC^T[ACGT]AGG
             2891  CCTCAGG             
EarI         3348  CTCTTC               CTCTTC(1/4)
             1979  GAAGAG              
Eco47III      730  AGCGCT               AGC^GCT
             1721  AGCGCT              
Eco81I        197  CCTGAGG              CC^T[ACGT]AGG
             2891  CCTCAGG             
KasI          188  GGCGCC               G^GCGCC
             2847  GGCGCC              
Ksp632I      3348  CTCTTC               CTCTTC(1/4)
             1979  GAAGAG              
MflI         2461  AGATCC               [GA]^GATC[CT]
             3877  GGATCT              
MslI         2832  CACAAGCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             3408  CACGTAAGTG          
NarI          188  GGCGCC               GG^CGCC
             2847  GGCGCC              
PaeR7I       1213  CTCGAG               C^TCGAG
             2429  CTCGAG              
PflFI        3465  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3768  GACTCCGTC           
PvuII        2283  CAGCTG               CAG^CTG
             2316  CAGCTG              
SfoI          188  GGCGCC               GGC^GCC
             2847  GGCGCC              
TfiI          383  GATTC                G^A[AT]TC
             2533  GATTC               
Tth111I      3465  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3768  GACTCCGTC           
XhoI         1213  CTCGAG               C^TCGAG
             2429  CTCGAG              
XhoII        2461  AGATCC               [GA]^GATC[CT]
             3877  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (  121 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbvCI       
BbvI        BcnI        BfaI        BglI        BmyI        
BpmI        BsaAI       BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1286I    BsrBI       BsrFI       BsrI        BssHII      
BssKI       BstF5I      BstNI       BstUI       BstXI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EagI        
EclXI       Eco52I      EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
KspI        MaeI        MaeII       MaeIII      MboI        
MboII       MluNI       MnlI        MscI        MseI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       PleI        PpuMI       PstI        RsaI        
SacII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfiI        SmaI        SmlI        StuI        
StyI        TaqI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        XmaCI       
XmaI