##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AclI AcsI AgeI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AvrII BciVI BclI
BlnI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssSI Bst1107I BstBI
BstZ17I ClaI DraI Eco47III EcoRI
EcoRV FbaI HindIII HpaI KpnI
MfeI MunI NdeI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI SalI
SbfI SfuI SgrAI SnaBI Sse8387I
SspBI SspI SwaI XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 728 TCCGGA T^CCGGA
AhdI 2142 GACACGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2142 GACACGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3869 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 402 TGCGCA TGC^GCA
BseAI 728 TCCGGA T^CCGGA
BspEI 728 TCCGGA T^CCGGA
BssHII 1120 GCGCGC G^CGCGC
BstAPI 405 GCACGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2456 GGTAACC G^GT[ACGT]ACC
BstPI 2456 GGTAACC G^GT[ACGT]ACC
CpoI 490 CGGACCG CG^G[AT]CCG
Eam1105I 2142 GACACGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2456 GGTAACC G^GT[ACGT]ACC
FspI 402 TGCGCA TGC^GCA
HincII 3764 GTCAAC GT[CT]^[GA]AC
HindII 3764 GTCAAC GT[CT]^[GA]AC
KspI 497 CCGCGG CCGC^GG
MluI 562 ACGCGT A^CGCGT
MroI 728 TCCGGA T^CCGGA
NheI 587 GCTAGC G^CTAGC
NotI 4587 GCGGCCGC GC^GGCCGC
PflFI 3869 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsrII 490 CGGACCG CG^G[AT]CCG
SacII 497 CCGCGG CCGC^GG
SapI 2360 GCTCTTC GCTCTTC(1/4)
ScaI 3495 AGTACT AGT^ACT
SfiI 1297 GGCCAGGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 3793 ACTAGT A^CTAGT
Tth111I 3869 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 1964 GTAGAC GT^[AC][GT]AC
4016 GTAGAC
AflII 481 CTTAAG C^TTAAG
2862 CTTAAG
Alw44I 1000 GTGCAC G^TGCAC
2676 GTGCAC
ApaLI 1000 GTGCAC G^TGCAC
2676 GTGCAC
BbeI 609 GGCGCC GGCGC^C
669 GGCGCC
BbrPI 406 CACGTG CAC^GTG
3689 CACGTG
BfrI 481 CTTAAG C^TTAAG
2862 CTTAAG
BglII 1844 AGATCT A^GATCT
3945 AGATCT
BlpI 95 GCTCAGC GC^T[ACGT]AGC
154 GCTGAGC
Bpu1102I 95 GCTCAGC GC^T[ACGT]AGC
154 GCTGAGC
BsaBI 166 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3940 GATGGAGATC
BspMI 4020 ACCTGC ACCTGC(4/8)
2924 GCAGGT
CelII 95 GCTCAGC GC^T[ACGT]AGC
154 GCTGAGC
EcoT22I 2516 ATGCAT ATGCA^T
2816 ATGCAT
FseI 789 GGCCGGCC GGCCGG^CC
1069 GGCCGGCC
KasI 609 GGCGCC G^GCGCC
669 GGCGCC
MamI 166 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3940 GATGGAGATC
NaeI 790 GCCGGC GCC^GGC
1070 GCCGGC
NarI 609 GGCGCC GG^CGCC
669 GGCGCC
NcoI 3543 CCATGG C^CATGG
4571 CCATGG
NgoMIV 790 GCCGGC G^CCGGC
1070 GCCGGC
NsiI 2516 ATGCAT ATGCA^T
2816 ATGCAT
PmaCI 406 CACGTG CAC^GTG
3689 CACGTG
PmlI 406 CACGTG CAC^GTG
3689 CACGTG
PstI 1095 CTGCAG CTGCA^G
3149 CTGCAG
SexAI 2472 ACCAGGT A^CC[AT]GGT
4479 ACCTGGT
SfoI 609 GGCGCC GGC^GCC
669 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvCI BbvI
BcgI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BsrBI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DrdI DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI