##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AclI AgeI AhdI Alw44I
ApaLI AscI AseI AsnI Asp700I
AspEI AvrII BamHI BclI BglII
BlnI BsaBI BsiWI BsmBI BspDI
BspHI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI Eam1105I EcoRI
EcoRV FbaI HincII HindII HindIII
HpaI MamI MfeI MluI MunI
NheI NruI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SalI SfuI SgrAI
SnaBI SpeI SwaI XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 559 GGTACC G^GTACC
AccIII 4406 TCCGGA T^CCGGA
AcsI 3437 GAATTT [GA]^AATT[CT]
AflII 2157 CTTAAG C^TTAAG
Aor51HI 2923 AGCGCT AGC^GCT
ApoI 3437 GAATTT [GA]^AATT[CT]
Asp718I 559 GGTACC G^GTACC
AspI 1116 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1519 TGGCCA TGG^CCA
BbrPI 4596 CACGTG CAC^GTG
BciVI 3952 GGATAC GTATCC(6/5)
BfrI 2157 CTTAAG C^TTAAG
BsaAI 4596 CACGTG [CT]AC^GT[GA]
BseAI 4406 TCCGGA T^CCGGA
BsmI 2959 GCATTC GAATGC(1/-1)
BspEI 4406 TCCGGA T^CCGGA
BspLU11I 4264 ACATGT A^CATGT
BsrDI 2492 CATTGC GCAATG(2/0)
BstEII 1146 GGTGACC G^GT[ACGT]ACC
BstPI 1146 GGTGACC G^GT[ACGT]ACC
DraI 4021 TTTAAA TTT^AAA
Eco47III 2923 AGCGCT AGC^GCT
EcoO65I 1146 GGTGACC G^GT[ACGT]ACC
EcoT22I 3685 ATGCAT ATGCA^T
FseI 1545 GGCCGGCC GGCCGG^CC
KpnI 559 GGTACC GGTAC^C
KspI 62 CCGCGG CCGC^GG
MluNI 1519 TGGCCA TGG^CCA
MroI 4406 TCCGGA T^CCGGA
MscI 1519 TGGCCA TGG^CCA
NdeI 3767 CATATG CA^TATG
NotI 4814 GCGGCCGC GC^GGCCGC
NsiI 3685 ATGCAT ATGCA^T
PflFI 1116 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 293 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4596 CACGTG CAC^GTG
PmlI 4596 CACGTG CAC^GTG
PshAI 761 GACGTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 62 CCGCGG CCGC^GG
SbfI 2207 CCTGCAGG CCTGCA^GG
ScaI 328 AGTACT AGT^ACT
SphI 4422 GCATGC GCATG^C
Sse8387I 2207 CCTGCAGG CCTGCA^GG
SspI 4624 AATATT AAT^ATT
Tth111I 1116 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 293 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AccI 312 GTCTAC GT^[AC][GT]AC
681 GTCTAC
AocI 1291 CCTCAGG CC^T[ACGT]AGG
2541 CCTGAGG
AviII 370 TGCGCA TGC^GCA
2857 TGCGCA
BcgI 2486 CGACCCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
847 GCATGGACTTCG
Bsu36I 1291 CCTCAGG CC^T[ACGT]AGG
2541 CCTGAGG
Eco81I 1291 CCTCAGG CC^T[ACGT]AGG
2541 CCTGAGG
FspI 370 TGCGCA TGC^GCA
2857 TGCGCA
SfiI 1296 GGCCGGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2109 GGCCCCTTTGGCC
SmlI 1567 CTCAAG C^T[CT][GA]AG
2157 CTTAAG
StuI 4328 AGGCCT AGG^CCT
4396 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrFI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DrdI DsaI EaeI
EagI EarI EclXI Eco52I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfoI SmaI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI