##################################
# NO CUT ENZYMES (   92 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcyI        
AflII       AhdI        Alw44I      ApaLI       AscI        
AspEI       AviII       AvrII       BalI        BbeI        
BbvCI       BcgI        BciVI       BcnI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaBI       
BsaHI       BseAI       BsiEI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BsrBI       BsrGI       
BssHII      BssSI       Bst1107I    BstEII      BstPI       
BstZ17I     CelII       ClaI        CpoI        DraIII      
EagI        Eam1105I    EclXI       Eco52I      EcoNI       
EcoO65I     EcoRV       FseI        FspI        HgaI        
Hin1I       HpaI        KasI        KspI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        NarI        NciI        NheI        NotI        
NruI        PmeI        PpuMI       Psp1406I    PvuI        
RsrII       SacII       SalI        SapI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        SspBI       SwaI        XbaI        XcmI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

Acc65I       3355  GGTACC               G^GTACC
AccII        2851  CGCG                 CG^CG
AgeI         2178  ACCGGT               A^CCGGT
Aor51HI       667  AGCGCT               AGC^GCT
ApaI         2162  GGGCCC               GGGCC^C
Asp718I      3355  GGTACC               G^GTACC
AspI          225  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          679  CTCGAG               C^[CT]CG[GA]G
BamHI        1824  GGATCC               G^GATCC
BbrPI         549  CACGTG               CAC^GTG
BglII        1940  AGATCT               A^GATCT
BsaI         1540  GAGACC               GGTCTC(1/5)
BsaWI        2178  ACCGGT               [AT]^CCGG[AT]
BsgI           55  CTGCAC               GTGCAG(16/14)
BsmFI        1386  GGGAC                GGGAC(10/14)
BsmI         2479  GCATTC               GAATGC(1/-1)
BsoBI         679  CTCGAG               C^[CT]CG[GA]G
BspLU11I     2902  ACATGT               A^CATGT
BstBI        2721  TTCGAA               TT^CGAA
BstUI        2851  CGCG                 CG^CG
CfoI          668  GCGC                 GCG^C
DrdI         2063  GACGAGCAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI         2024  TGGCCG               [CT]^GGCC[GA]
Eco47III      667  AGCGCT               AGC^GCT
EcoRI        2972  GAATTC               G^AATTC
FauI         2165  CCCGC                CCCGC(4/6)
HaeII         667  AGCGCT               [GA]GCGC^[CT]
HhaI          668  GCGC                 GCG^C
HinP1I        668  GCGC                 G^CGC
HincII       2741  GTTGAC               GT[CT]^[GA]AC
HindII       2741  GTTGAC               GT[CT]^[GA]AC
HindIII       273  AAGCTT               A^AGCTT
KpnI         3355  GGTACC               GGTAC^C
MspA1I       1953  CAGCTG               C[AC]G^C[GT]G
MvnI         2851  CGCG                 CG^CG
NaeI         2026  GCCGGC               GCC^GGC
NdeI         2286  CATATG               CA^TATG
NgoMIV       2026  GCCGGC               G^CCGGC
NspV         2721  TTCGAA               TT^CGAA
PacI         3086  TTAATTAA             TTAAT^TAA
PaeR7I        679  CTCGAG               C^TCGAG
PflFI         225  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        2178  ACCGGT               A^CCGGT
PmaCI         549  CACGTG               CAC^GTG
PmlI          549  CACGTG               CAC^GTG
PshAI        1898  GACCACCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII        1953  CAGCTG               CAG^CTG
SacI         3373  GAGCTC               GAGCT^C
SbfI         1097  CCTGCAGG             CCTGCA^GG
ScaI         3541  AGTACT               AGT^ACT
SfuI         2721  TTCGAA               TT^CGAA
Sse8387I     1097  CCTGCAGG             CCTGCA^GG
SspI         3607  AATATT               AAT^ATT
Tth111I       225  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          679  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AocI          264  CCTGAGG              CC^T[ACGT]AGG
              981  CCTGAGG             
BanI          156  GGTGCC               G^G[CT][GA]CC
             3355  GGTACC              
BanII        2162  GGGCCC               G[GA]GC[CT]^C
             3373  GAGCTC              
BbsI           17  GAAGAC               GAAGAC(2/6)
              259  GAAGAC              
BbvI         1028  GCAGC                GCAGC(8/12)
             1955  GCTGC               
BclI          440  TGATCA               T^GATCA
             2640  TGATCA              
BpuAI          17  GAAGAC               GAAGAC(2/6)
              259  GAAGAC              
BsaAI         194  TACGTG               [CT]AC^GT[GA]
              549  CACGTG              
BsrFI        2026  GCCGGC               [GA]^CCGG[CT]
             2178  ACCGGT              
BstAPI        589  GCAACCTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              950  GCAGACTCTGC         
BstXI        1579  CCATTGGCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1984  CCATGGAGATGG        
BstYI        1824  GGATCC               [GA]^GATC[CT]
             1940  AGATCT              
Bsu36I        264  CCTGAGG              CC^T[ACGT]AGG
              981  CCTGAGG             
Cfr10I       2026  GCCGGC               [GA]^CCGG[CT]
             2178  ACCGGT              
DraI         3006  TTTAAA               TTT^AAA
             3522  TTTAAA              
DraII         628  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              811  AGGGCCT             
EarI         2512  CTCTTC               CTCTTC(1/4)
             2792  CTCTTC              
Eco81I        264  CCTGAGG              CC^T[ACGT]AGG
              981  CCTGAGG             
EcoO109I      628  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              811  AGGGCCT             
FbaI          440  TGATCA               T^GATCA
             2640  TGATCA              
HapII        2027  CCGG                 C^CGG
             2179  CCGG                
HpaII        2027  CCGG                 C^CGG
             2179  CCGG                
Ksp632I      2512  CTCTTC               CTCTTC(1/4)
             2792  CTCTTC              
MflI         1824  GGATCC               [GA]^GATC[CT]
             1940  AGATCT              
MspI         2027  CCGG                 C^CGG
             2179  CCGG                
PflMI        1828  CCAATCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1952  CCAGCTGCTGG         
PstI          948  CTGCAG               CTGCA^G
             1098  CTGCAG              
StuI         1094  AGGCCT               AGG^CCT
             2009  AGGCCT              
TseI         1028  GCAGC                G^C[AT]GC
             1955  GCTGC               
Van91I       1828  CCAATCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1952  CCAGCTGCTGG         
XhoII        1824  GGATCC               [GA]^GATC[CT]
             1940  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        AseI        AsnI        
Asp700I     AspHI       AvaII       BfaI        BmyI        
BpmI        BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       Bsp1286I    BspHI       BspMI       
BsrDI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       DsaI        
EcoRII      EcoT14I     EcoT22I     Fnu4HI      FokI        
HaeIII      HinfI       HphI        ItaI        MaeI        
MaeII       MaeIII      MboI        MboII       MnlI        
MseI        MslI        MvaI        MwoI        NcoI        
NdeII       NlaIII      NlaIV       NsiI        NspI        
PleI        PshBI       RcaI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SphI        StyI        TaqI        TfiI        Tru9I       
Tsp45I      Tsp509I     TspRI       TthHB8I     XmnI