##################################
# NO CUT ENZYMES (   80 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AgeI        
AhdI        Alw44I      Aor51HI     ApaLI       AscI        
Asp718I     AspEI       AviII       AvrII       BamHI       
BbeI        BcgI        BciVI       BlnI        BsaBI       
BsiEI       BsiWI       Bsp1407I    BspDI       BspLU11I    
BsrFI       BsrGI       BssHII      Bst1107I    BstBI       
BstUI       BstZ17I     Cfr10I      ClaI        CpoI        
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoRI       FseI        FspI        HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MluI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NotI        NruI        NspV        PacI        
PaeR7I      PinAI       PmeI        Psp1406I    PvuI        
RsrII       SacII       SapI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SpeI        
SphI        Sse8387I    SspBI       SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccIII       1311  TCCGGA               T^CCGGA
AcyI         3487  GACGCC               G[GA]^CG[CT]C
AflIII       2338  ACGTGT               A^C[GA][CT]GT
AocI          815  CCTTAGG              CC^T[ACGT]AGG
BglI         1588  GCCAGAAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        4308  AGATCT               A^GATCT
BlpI         1861  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1861  GCTCAGC              GC^T[ACGT]AGC
BsaHI        3487  GACGCC               G[GA]^CG[CT]C
BseAI        1311  TCCGGA               T^CCGGA
BseRI        4688  CTCCTC               GAGGAG(10/8)
BspEI        1311  TCCGGA               T^CCGGA
BspHI        1554  TCATGA               T^CATGA
BsrBI         684  GAGCGG               CCGCTC(-3/-3)
BsrDI         614  GCAATG               GCAATG(2/0)
BstAPI       4100  GCAGAGATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       4572  GGTTACC              G^GT[ACGT]ACC
BstPI        4572  GGTTACC              G^GT[ACGT]ACC
Bsu36I        815  CCTTAGG              CC^T[ACGT]AGG
CelII        1861  GCTCAGC              GC^T[ACGT]AGC
DsaI         5209  CCATGG               C^C[GA][CT]GG
Eco81I        815  CCTTAGG              CC^T[ACGT]AGG
EcoNI        1672  CCTGATTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      4572  GGTTACC              G^GT[ACGT]ACC
EcoRV        1732  GATATC               GAT^ATC
HaeII        2816  AGCGCC               [GA]GCGC^[CT]
HgaI         3487  GACGC                GACGC(5/10)
Hin1I        3487  GACGCC               G[GA]^CG[CT]C
MfeI         1406  CAATTG               C^AATTG
MroI         1311  TCCGGA               T^CCGGA
MunI         1406  CAATTG               C^AATTG
NcoI         5209  CCATGG               C^CATGG
NheI         3270  GCTAGC               G^CTAGC
NspI         4029  GCATGT               [GA]CATG^[CT]
PshAI        3487  GACGCCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         1554  TCATGA               T^CATGA
SalI         4146  GTCGAC               G^TCGAC
SnaBI        4235  TACGTA               TAC^GTA
XcmI         1716  CCAGGTGGCTTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccI         4146  GTCGAC               GT^[AC][GT]AC
             4600  GTAGAC              
AflII         508  CTTAAG               C^TTAAG
             5197  CTTAAG              
ApaI          673  GGGCCC               GGGCC^C
             5050  GGGCCC              
AseI         1467  ATTAAT               AT^TAAT
             2740  ATTAAT              
AsnI         1467  ATTAAT               AT^TAAT
             2740  ATTAAT              
BbvCI        3452  GCTGAGG              CCTCAGC(-5/-2)
             4068  GCTGAGG             
BclI         1551  TGATCA               T^GATCA
             2570  TGATCA              
BfrI          508  CTTAAG               C^TTAAG
             5197  CTTAAG              
BsaWI        1311  TCCGGA               [AT]^CCGG[AT]
             1891  ACCGGA              
BsmBI        3998  CGTCTC               CGTCTC(1/5)
             4164  CGTCTC              
BssSI        3951  CACGAG               CACGAG(-5/-1)
             1144  CTCGTG              
BstXI        3920  CCAGCACTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4053  CCACCTACTTGG        
DraIII        652  CACCACGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4978  CACATTGTG           
EcoT22I       491  ATGCAT               ATGCA^T
             4227  ATGCAT              
FbaI         1551  TGATCA               T^GATCA
             2570  TGATCA              
NsiI          491  ATGCAT               ATGCA^T
             4227  ATGCAT              
PpuMI         503  AGGACCT              [GA]G^G[AT]CC[CT]
             5215  AGGTCCC             
PshBI        1467  ATTAAT               AT^TAAT
             2740  ATTAAT              
PstI         1069  CTGCAG               CTGCA^G
             2461  CTGCAG              
SacI          559  GAGCTC               GAGCT^C
             1198  GAGCTC              
SmaI         1059  CCCGGG               CCC^GGG
             2606  CCCGGG              
XbaI         3107  TCTAGA               T^CTAGA
             3848  TCTAGA              
XmaCI        1059  CCCGGG               C^CCGGG
             2606  CCCGGG              
XmaI         1059  CCCGGG               C^CCGGG
             2606  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  115 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        Asp700I     AspHI       AspI        
AvaI        AvaII       BalI        BanI        BanII       
BbrPI       BbsI        BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaAI       BsaI        
BsaJI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraI        DraII       EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PflFI       PflMI       PleI        PmaCI       
PmlI        PvuII       RsaI        Sau3AI      Sau96I      
ScaI        ScrFI       SfaNI       SfcI        SmlI        
SspI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Tth111I     TthHB8I     Van91I      XhoII       XmnI