##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AgeI        AhdI        AocI        ApaI        AscI        
Asp718I     AspEI       AspI        AvrII       BanI        
BbeI        BcgI        BcnI        BglI        BlnI        
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsiEI       BsiWI       BspDI       BspEI       
BspMI       BsrBI       BsrFI       BssHII      BssSI       
Bst1107I    BstBI       BstZ17I     Bsu36I      Cfr10I      
ClaI        CpoI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco52I      Eco81I      EcoNI       
EcoRI       FseI        HapII       HgaI        Hin1I       
HpaII       KasI        KpnI        MamI        MluI        
MroI        MspI        NaeI        NarI        NciI        
NgoMIV      NheI        NotI        NruI        NspV        
PflFI       PinAI       PmeI        PshAI       Psp1406I    
PvuI        RsrII       SalI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SphI        Sse8387I    StuI        SwaI        
Tth111I     XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccII        1755  CGCG                 CG^CG
AflII        1031  CTTAAG               C^TTAAG
Alw44I       1844  GTGCAC               G^TGCAC
AlwNI        1610  CAGCTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       657  AGCGCT               AGC^GCT
ApaLI        1844  GTGCAC               G^TGCAC
AvaI         2301  CTCGAG               C^[CT]CG[GA]G
AviII        4321  TGCGCA               TGC^GCA
BalI         2781  TGGCCA               TGG^CCA
BbrPI          20  CACGTG               CAC^GTG
BbvCI         820  GCTGAGG              CCTCAGC(-5/-2)
BfrI         1031  CTTAAG               C^TTAAG
BglII        3647  AGATCT               A^GATCT
BsgI          809  GTGCAG               GTGCAG(16/14)
BsmBI        4697  CGTCTC               CGTCTC(1/5)
BsoBI        2301  CTCGAG               C^[CT]CG[GA]G
Bsp1407I     3780  TGTACA               T^GTACA
BspLU11I     1098  ACATGT               A^CATGT
BsrGI        3780  TGTACA               T^GTACA
BstEII        826  GGTGACC              G^GT[ACGT]ACC
BstPI         826  GGTGACC              G^GT[ACGT]ACC
BstUI        1755  CGCG                 CG^CG
BstXI        4306  CCACTCATTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII        4521  AGGACCT              [GA]G^G[ACGT]CC[CT]
EaeI         2781  TGGCCA               [CT]^GGCC[GA]
Eco47III      657  AGCGCT               AGC^GCT
EcoO109I     4521  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       826  GGTGACC              G^GT[ACGT]ACC
FspI         4321  TGCGCA               TGC^GCA
HpaI         2630  GTTAAC               GTT^AAC
KspI         1754  CCGCGG               CCGC^GG
MfeI          627  CAATTG               C^AATTG
MluNI        2781  TGGCCA               TGG^CCA
MscI         2781  TGGCCA               TGG^CCA
MunI          627  CAATTG               C^AATTG
MvnI         1755  CGCG                 CG^CG
NcoI         1180  CCATGG               C^CATGG
NdeI          713  CATATG               CA^TATG
PacI         2387  TTAATTAA             TTAAT^TAA
PaeR7I       2301  CTCGAG               C^TCGAG
PflMI        1240  CCATAATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI          20  CACGTG               CAC^GTG
PmlI           20  CACGTG               CAC^GTG
PpuMI        4521  AGGACCT              [GA]G^G[AT]CC[CT]
SacI         3531  GAGCTC               GAGCT^C
SacII        1754  CCGCGG               CCGC^GG
ScaI         1108  AGTACT               AGT^ACT
SspBI        3780  TGTACA               T^GTACA
Van91I       1240  CCATAATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         3798  CCAGGAATCTTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         2301  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AccI         1463  GTCTAC               GT^[AC][GT]AC
             2254  GTAGAC              
BanII         121  GGGCTC               G[GA]GC[CT]^C
             3531  GAGCTC              
BciVI        1687  GGATAC               GTATCC(6/5)
             1927  GGATAC              
BlpI         1801  GCTGAGC              GC^T[ACGT]AGC
             3920  GCTCAGC             
Bpu1102I     1801  GCTGAGC              GC^T[ACGT]AGC
             3920  GCTCAGC             
BsaAI          20  CACGTG               [CT]AC^GT[GA]
             4387  TACGTG              
BspHI         466  TCATGA               T^CATGA
             2933  TCATGA              
Cac8I         119  GCGGGC               GC[ACGT]^[ACGT]GC
             2065  GCCTGC              
CelII        1801  GCTGAGC              GC^T[ACGT]AGC
             3920  GCTCAGC             
EcoRV         272  GATATC               GAT^ATC
              282  GATATC              
FauI          119  GCGGG                CCCGC(4/6)
             1756  GCGGG               
HaeII         583  AGCGCC               [GA]GCGC^[CT]
              657  AGCGCT              
PstI         2067  CTGCAG               CTGCA^G
             3466  CTGCAG              
RcaI          466  TCATGA               T^CATGA
             2933  TCATGA              
SapI         3236  GAAGAGC              GCTCTTC(1/4)
             4814  GCTCTTC             
SpeI           35  ACTAGT               A^CTAGT
              943  ACTAGT              


#########################################
# 3 OR MORE CUT ENZYMES (   97 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        ApoI        AseI        AsnI        Asp700I     
AspHI       AvaII       BamHI       BbsI        BbvI        
BclI        BfaI        BmyI        BpmI        BpuAI       
BsaJI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    BsrDI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstYI       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DsaI        EarI        EcoRII      EcoT14I     EcoT22I     
FbaI        Fnu4HI      FokI        HaeIII      HhaI        
HinP1I      HincII      HindII      HindIII     HinfI       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MvaI        MwoI        
NdeII       NlaIII      NlaIV       NsiI        NspI        
PleI        PshBI       PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       XmnI