##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI Aor51HI AscI AviII AvrII
BamHI BbeI BbrPI BbvCI BfrI
BlnI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BspEI BsrBI BsrFI
BssHII Bst1107I BstZ17I Cfr10I CpoI
EagI EclXI Eco47III Eco52I FseI
FspI Hin1I HpaI KasI KspI
MfeI MluI MroI MunI NaeI
NarI NgoMIV NheI NotI NruI
PacI PaeR7I PinAI PmaCI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SphI Sse8387I
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 154 GTCTAC GT^[AC][GT]AC
AhdI 982 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2337 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1855 CCTGAGG CC^T[ACGT]AGG
Asp700I 3132 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 982 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 3114 TGGCCA TGG^CCA
BbsI 2729 GAAGAC GAAGAC(2/6)
BglI 2060 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 2729 GAAGAC GAAGAC(2/6)
Bsp1407I 4603 TGTACA T^GTACA
BspDI 3913 ATCGAT AT^CGAT
BspLU11I 3548 ACATGT A^CATGT
BspMI 1283 ACCTGC ACCTGC(4/8)
BsrGI 4603 TGTACA T^GTACA
Bsu36I 1855 CCTGAGG CC^T[ACGT]AGG
ClaI 3913 ATCGAT AT^CGAT
DrdI 693 GACTTTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 982 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1855 CCTGAGG CC^T[ACGT]AGG
EcoNI 4453 CCTAAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 4292 GATATC GAT^ATC
FauI 3149 GCGGG CCCGC(4/6)
HaeII 3215 AGCGCC [GA]GCGC^[CT]
MluNI 3114 TGGCCA TGG^CCA
MscI 3114 TGGCCA TGG^CCA
MspA1I 2775 CAGCTG C[AC]G^C[GT]G
PmeI 4824 GTTTAAAC GTTT^AAAC
PvuII 2775 CAGCTG CAG^CTG
SapI 176 GCTCTTC GCTCTTC(1/4)
SmlI 1413 CTTGAG C^T[CT][GA]AG
SnaBI 1097 TACGTA TAC^GTA
SpeI 4156 ACTAGT A^CTAGT
SspBI 4603 TGTACA T^GTACA
StuI 2491 AGGCCT AGG^CCT
XbaI 4793 TCTAGA T^CTAGA
XcmI 1602 CCAGGCTTCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3132 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 1187 GGTACC G^GTACC
3346 GGTACC
AccII 1307 CGCG CG^CG
1324 CGCG
ApaI 1659 GGGCCC GGGCC^C
2972 GGGCCC
AseI 324 ATTAAT AT^TAAT
439 ATTAAT
AsnI 324 ATTAAT AT^TAAT
439 ATTAAT
Asp718I 1187 GGTACC G^GTACC
3346 GGTACC
AspI 2550 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3804 GACAAAGTC
AvaI 520 CCCGAG C^[CT]CG[GA]G
2969 CTCGGG
BcgI 201 GCATGAGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2989 GCAGCCCATTCG
BciVI 1236 GGATAC GTATCC(6/5)
468 GTATCC
BclI 494 TGATCA T^GATCA
4675 TGATCA
BcnI 128 CCGGG CC^[GC]GG
1210 CCGGG
BlpI 1561 GCTAAGC GC^T[ACGT]AGC
2684 GCTGAGC
Bpu1102I 1561 GCTAAGC GC^T[ACGT]AGC
2684 GCTGAGC
BsaAI 1097 TACGTA [CT]AC^GT[GA]
2317 CACGTA
BsaBI 489 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2257 GATCCTTATC
BsmI 4203 GAATGC GAATGC(1/-1)
3076 GCATTC
BsoBI 520 CCCGAG C^[CT]CG[GA]G
2969 CTCGGG
BstAPI 192 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1536 GCAAGTGTTGC
BstBI 1034 TTCGAA TT^CGAA
1253 TTCGAA
BstEII 793 GGTTACC G^GT[ACGT]ACC
1369 GGTTACC
BstPI 793 GGTTACC G^GT[ACGT]ACC
1369 GGTTACC
BstUI 1307 CGCG CG^CG
1324 CGCG
CelII 1561 GCTAAGC GC^T[ACGT]AGC
2684 GCTGAGC
DraII 13 GGGTCCC [GA]G^G[ACGT]CC[CT]
1272 AGGACCC
DraIII 2507 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2690 CACGCTGTG
EaeI 1745 CGGCCA [CT]^GGCC[GA]
3114 TGGCCA
EcoO109I 13 GGGTCCC [GA]G^G[ACGT]CC[CT]
1272 AGGACCC
EcoO65I 793 GGTTACC G^GT[ACGT]ACC
1369 GGTTACC
EcoT22I 199 ATGCAT ATGCA^T
1227 ATGCAT
FbaI 494 TGATCA T^GATCA
4675 TGATCA
HgaI 2193 GACGC GACGC(5/10)
1566 GCGTC
HincII 1400 GTCAAC GT[CT]^[GA]AC
3160 GTTGAC
HindII 1400 GTCAAC GT[CT]^[GA]AC
3160 GTTGAC
KpnI 1187 GGTACC GGTAC^C
3346 GGTACC
MamI 489 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2257 GATCCTTATC
MvnI 1307 CGCG CG^CG
1324 CGCG
NciI 128 CCGGG CC^[GC]GG
1210 CCGGG
NcoI 789 CCATGG C^CATGG
3111 CCATGG
NdeI 839 CATATG CA^TATG
4607 CATATG
NsiI 199 ATGCAT ATGCA^T
1227 ATGCAT
NspV 1034 TTCGAA TT^CGAA
1253 TTCGAA
PflFI 2550 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3804 GACAAAGTC
PflMI 1052 CCAAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1748 CCAGAGCGTGG
PpuMI 13 GGGTCCC [GA]G^G[AT]CC[CT]
1272 AGGACCC
PshBI 324 ATTAAT AT^TAAT
439 ATTAAT
SacI 2332 GAGCTC GAGCT^C
2966 GAGCTC
SfaNI 1632 GATGC GCATC(5/9)
371 GCATC
SfuI 1034 TTCGAA TT^CGAA
1253 TTCGAA
SspI 4748 AATATT AAT^ATT
4802 AATATT
Tth111I 2550 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3804 GACAAAGTC
Van91I 1052 CCAAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1748 CCAGAGCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI ApaLI ApoI AspHI
AvaII BanI BanII BbvI BfaI
BglII BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI Bsp1286I BspHI BsrDI
BsrI BssKI BssSI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DsaI EarI
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PstI RcaI RsaI
Sau3AI Sau96I ScrFI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII