##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AccIII AflII AgeI AscI Asp700I
AspI AvrII BbeI BcgI BclI
BfrI BlnI BsaBI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BsrGI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DrdI FbaI FseI HindIII
KasI MamI MluI MroI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PflFI PinAI PmeI
RsrII SalI SbfI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 3738 GGTACC G^GTACC
AccI 2351 GTAGAC GT^[AC][GT]AC
AclI 4884 AACGTT AA^CGTT
AhdI 4453 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 947 GTGCAC G^TGCAC
Aor51HI 1053 AGCGCT AGC^GCT
ApaLI 947 GTGCAC G^TGCAC
AseI 4915 ATTAAT AT^TAAT
AsnI 4915 ATTAAT AT^TAAT
Asp718I 3738 GGTACC G^GTACC
AspEI 4453 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1539 TGCGCA TGC^GCA
BbrPI 1850 CACGTG CAC^GTG
BlpI 563 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 563 GCTGAGC GC^T[ACGT]AGC
BsaAI 1850 CACGTG [CT]AC^GT[GA]
BspHI 208 TCATGA T^CATGA
BstEII 2110 GGTCACC G^GT[ACGT]ACC
BstPI 2110 GGTCACC G^GT[ACGT]ACC
CelII 563 GCTGAGC GC^T[ACGT]AGC
EagI 3881 CGGCCG C^GGCCG
Eam1105I 4453 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3881 CGGCCG C^GGCCG
Eco47III 1053 AGCGCT AGC^GCT
Eco52I 3881 CGGCCG C^GGCCG
EcoO65I 2110 GGTCACC G^GT[ACGT]ACC
EcoRV 2342 GATATC GAT^ATC
EcoT22I 1799 ATGCAT ATGCA^T
FspI 1539 TGCGCA TGC^GCA
HpaI 4881 GTTAAC GTT^AAC
KpnI 3738 GGTACC GGTAC^C
KspI 3845 CCGCGG CCGC^GG
MfeI 4738 CAATTG C^AATTG
MunI 4738 CAATTG C^AATTG
NdeI 2031 CATATG CA^TATG
NsiI 1799 ATGCAT ATGCA^T
PmaCI 1850 CACGTG CAC^GTG
PmlI 1850 CACGTG CAC^GTG
PshAI 1064 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4915 ATTAAT AT^TAAT
Psp1406I 4884 AACGTT AA^CGTT
PvuI 1981 CGATCG CGAT^CG
RcaI 208 TCATGA T^CATGA
SacII 3845 CCGCGG CCGC^GG
SapI 3994 GAAGAGC GCTCTTC(1/4)
SexAI 570 ACCTGGT A^CC[AT]GGT
XbaI 4255 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AatII 53 GACGTC GACGT^C
718 GACGTC
ApaI 1351 GGGCCC GGGCC^C
3832 GGGCCC
BbsI 380 GAAGAC GAAGAC(2/6)
1307 GAAGAC
BciVI 4288 GGATAC GTATCC(6/5)
2686 GTATCC
BglII 1788 AGATCT A^GATCT
3456 AGATCT
BpuAI 380 GAAGAC GAAGAC(2/6)
1307 GAAGAC
BsaWI 2949 ACCGGA [AT]^CCGG[AT]
3255 ACCGGA
BsiEI 1981 CGATCG CG[GA][CT]^CG
3881 CGGCCG
BsmBI 51 GAGACG CGTCTC(1/5)
716 GAGACG
BspLU11I 133 ACATGT A^CATGT
3651 ACATGT
BspMI 951 ACCTGC ACCTGC(4/8)
3197 ACCTGC
BsrDI 1897 CATTGC GCAATG(2/0)
4585 GCAATG
BsrFI 2043 ACCGGC [GA]^CCGG[CT]
2550 ACCGGC
Cfr10I 2043 ACCGGC [GA]^CCGG[CT]
2550 ACCGGC
DraI 4766 TTTAAA TTT^AAA
5112 TTTAAA
DraIII 569 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
5479 CACTCCGTG
EcoRI 4976 GAATTC G^AATTC
5251 GAATTC
PaeR7I 2018 CTCGAG C^TCGAG
5382 CTCGAG
PflMI 932 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1075 CCAGTCCCTGG
SacI 390 GAGCTC GAGCT^C
1343 GAGCTC
ScaI 495 AGTACT AGT^ACT
1380 AGTACT
SfiI 2074 GGCCAACTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3914 GGCCGTGGTGGCC
SmaI 1485 CCCGGG CCC^GGG
3208 CCCGGG
SphI 2157 GCATGC GCATG^C
2379 GCATGC
StuI 2526 AGGCCT AGG^CCT
4276 AGGCCT
Van91I 932 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1075 CCAGTCCCTGG
XhoI 2018 CTCGAG C^TCGAG
5382 CTCGAG
XmaCI 1485 CCCGGG C^CCGGG
3208 CCCGGG
XmaI 1485 CCCGGG C^CCGGG
3208 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII BalI
BamHI BanI BanII BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII