##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AgeI        
Alw44I      ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     BamHI       BciVI       BfrI        
BseAI       BsiWI       BsmBI       BspDI       BspEI       
BsrBI       BsrFI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     Cfr10I      ClaI        
CpoI        DraIII      EagI        EclXI       Eco52I      
EcoO65I     FseI        HpaI        KpnI        MluI        
MroI        NaeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PinAI       
PmeI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RsrII       SalI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SnaBI       Sse8387I    StuI        SwaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

AatII        2047  GACGTC               GACGT^C
AccI         1113  GTAGAC               GT^[AC][GT]AC
AhdI          321  GACACACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      2031  AGCGCT               AGC^GCT
AspEI         321  GACACACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1811  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI           46  CTCGGG               C^[CT]CG[GA]G
AviII        2346  TGCGCA               TGC^GCA
AvrII        3127  CCTAGG               C^CTAGG
BalI         4265  TGGCCA               TGG^CCA
BbeI           68  GGCGCC               GGCGC^C
BbrPI         841  CACGTG               CAC^GTG
BcgI         2061  CGACTGTGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI         4267  GCCAGGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         856  AGATCT               A^GATCT
BlnI         3127  CCTAGG               C^CTAGG
BlpI           79  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I       79  GCTGAGC              GC^T[ACGT]AGC
BsaAI         841  CACGTG               [CT]AC^GT[GA]
BsaBI        1093  GATCAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          939  GGTCTC               GGTCTC(1/5)
BsiEI          40  CGGTCG               CG[GA][CT]^CG
BsoBI          46  CTCGGG               C^[CT]CG[GA]G
Bsp1407I     2145  TGTACA               T^GTACA
BsrGI        2145  TGTACA               T^GTACA
BssSI         860  CTCGTG               CACGAG(-5/-1)
CelII          79  GCTGAGC              GC^T[ACGT]AGC
DraII         602  AGGGCCT              [GA]G^G[ACGT]CC[CT]
DrdI         3772  GACACTTTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI         4265  TGGCCA               [CT]^GGCC[GA]
Eam1105I      321  GACACACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     2031  AGCGCT               AGC^GCT
EcoO109I      602  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoRV        1244  GATATC               GAT^ATC
EcoT22I      2747  ATGCAT               ATGCA^T
FspI         2346  TGCGCA               TGC^GCA
HgaI         1664  GACGC                GACGC(5/10)
HincII       2161  GTTGAC               GT[CT]^[GA]AC
HindII       2161  GTTGAC               GT[CT]^[GA]AC
HindIII       383  AAGCTT               A^AGCTT
KasI           68  GGCGCC               G^GCGCC
KspI           72  CCGCGG               CCGC^GG
MamI         1093  GATCAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         3503  CAATTG               C^AATTG
MluNI        4265  TGGCCA               TGG^CCA
MscI         4265  TGGCCA               TGG^CCA
MunI         3503  CAATTG               C^AATTG
NarI           68  GGCGCC               GG^CGCC
NdeI         3847  CATATG               CA^TATG
NsiI         2747  ATGCAT               ATGCA^T
PflFI        1811  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         841  CACGTG               CAC^GTG
PmlI          841  CACGTG               CAC^GTG
SacII          72  CCGCGG               CCGC^GG
SfoI           68  GGCGCC               GGC^GCC
SphI         4604  GCATGC               GCATG^C
SspBI        2145  TGTACA               T^GTACA
SspI         3856  AATATT               AAT^ATT
Tth111I      1811  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         4443  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AocI         1959  CCTCAGG              CC^T[ACGT]AGG
             3479  CCTCAGG             
BbvCI        4307  CCTCAGC              CCTCAGC(-5/-2)
             1853  GCTGAGG             
BclI          573  TGATCA               T^GATCA
             1740  TGATCA              
BsaWI         139  ACCGGA               [AT]^CCGG[AT]
             2542  TCCGGT              
BsmI          580  GAATGC               GAATGC(1/-1)
             1218  GAATGC              
BspHI        1105  TCATGA               T^CATGA
             3180  TCATGA              
BstAPI         89  GCAGACATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             4680  GCATGTGCTGC         
BstXI        4152  CCAGGAGATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4358  CCATTGGCCTGG        
BstYI         856  AGATCT               [GA]^GATC[CT]
             3444  GGATCT              
Bsu36I       1959  CCTCAGG              CC^T[ACGT]AGG
             3479  CCTCAGG             
Eco81I       1959  CCTCAGG              CC^T[ACGT]AGG
             3479  CCTCAGG             
EcoRI         836  GAATTC               G^AATTC
             4060  GAATTC              
FbaI          573  TGATCA               T^GATCA
             1740  TGATCA              
HaeII          68  GGCGCC               [GA]GCGC^[CT]
             2031  AGCGCT              
MflI          856  AGATCT               [GA]^GATC[CT]
             3444  GGATCT              
PflMI         919  CCACTGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1939  CCATTCCATGG         
RcaI         1105  TCATGA               T^CATGA
             3180  TCATGA              
SacI          184  GAGCTC               GAGCT^C
             1792  GAGCTC              
SapI          714  GAAGAGC              GCTCTTC(1/4)
             1693  GAAGAGC             
SmlI          731  CTTGAG               C^T[CT][GA]AG
             1138  CTTGAG              
SpeI         4195  ACTAGT               A^CTAGT
             4660  ACTAGT              
Van91I        919  CCACTGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1939  CCATTCCATGG         
XhoII         856  AGATCT               [GA]^GATC[CT]
             3444  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (  103 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       ApoI        
Asp700I     AspHI       AvaII       BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaHI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspLU11I    BspMI       BsrDI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        DsaI        EarI        EcoNI       EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PleI        PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XcmI        XmnI