##################################
# NO CUT ENZYMES (   69 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AgeI        
AhdI        Aor51HI     AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AviII       BbrPI       
BclI        BfrI        BglII       BlpI        Bpu1102I    
BseAI       BsiWI       BsmI        BspDI       BspEI       
BsrDI       BstEII      BstPI       CelII       ClaI        
CpoI        Eam1105I    Eco47III    EcoNI       EcoO65I     
EcoRV       EcoT22I     FbaI        FseI        FspI        
HindIII     MfeI        MluI        MroI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    RsrII       
SalI        ScaI        SfiI        SnaBI       SwaI        
Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AvrII        3893  CCTAGG               C^CTAGG
BbeI          613  GGCGCC               GGCGC^C
BcgI         3771  CGAAGACTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI         3893  CCTAGG               C^CTAGG
BsaAI        2722  TACGTG               [CT]AC^GT[GA]
BsaBI        2026  GATATGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        3152  ACCGGA               [AT]^CCGG[AT]
BstBI        3769  TTCGAA               TT^CGAA
DraIII       4601  CACCGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
HpaI          884  GTTAAC               GTT^AAC
KasI          613  GGCGCC               G^GCGCC
MamI         2026  GATATGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI         2066  GCCGGC               GCC^GGC
NarI          613  GGCGCC               GG^CGCC
NgoMIV       2066  GCCGGC               G^CCGGC
NspV         3769  TTCGAA               TT^CGAA
PflMI        3324  CCACAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI          95  GACAAGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI         3864  CGATCG               CGAT^CG
SbfI         2490  CCTGCAGG             CCTGCA^GG
SexAI        1871  ACCAGGT              A^CC[AT]GGT
SfoI          613  GGCGCC               GGC^GCC
SfuI         3769  TTCGAA               TT^CGAA
SgrAI        1624  CACCGGCG             C[GA]^CCGG[CT]G
SmlI         4453  CTCAAG               C^T[CT][GA]AG
SpeI         4168  ACTAGT               A^CTAGT
SphI         3132  GCATGC               GCATG^C
Sse8387I     2490  CCTGCAGG             CCTGCA^GG
SspI         4231  AATATT               AAT^ATT
Van91I       3324  CCACAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

Acc65I        198  GGTACC               G^GTACC
              525  GGTACC              
AccI         2466  GTATAC               GT^[AC][GT]AC
             5162  GTATAC              
Alw44I       2932  GTGCAC               G^TGCAC
             3286  GTGCAC              
AocI          214  CCTTAGG              CC^T[ACGT]AGG
             3998  CCTCAGG             
ApaI          294  GGGCCC               GGGCC^C
              437  GGGCCC              
ApaLI        2932  GTGCAC               G^TGCAC
             3286  GTGCAC              
Asp718I       198  GGTACC               G^GTACC
              525  GGTACC              
BbvCI        3726  CCTCAGC              CCTCAGC(-5/-2)
             2887  GCTGAGG             
BglI         2759  GCCTGAGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             4074  GCCAGCTGGGC         
BsaI         3538  GAGACC               GGTCTC(1/5)
             4596  GGTCTC              
Bsp1407I     3817  TGTACA               T^GTACA
             4633  TGTACA              
BspHI        3410  TCATGA               T^CATGA
             5168  TCATGA              
BspLU11I     1664  ACATGT               A^CATGT
             4947  ACATGT              
BsrGI        3817  TGTACA               T^GTACA
             4633  TGTACA              
Bst1107I     2466  GTATAC               GTA^TAC
             5162  GTATAC              
BstAPI       3108  GCACCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             4819  GCACCCCCTGC         
BstZ17I      2466  GTATAC               GTA^TAC
             5162  GTATAC              
Bsu36I        214  CCTTAGG              CC^T[ACGT]AGG
             3998  CCTCAGG             
DraI         1052  TTTAAA               TTT^AAA
             4141  TTTAAA              
DrdI         1670  GACGAGCCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2488  GACCTGCAGGTC        
Eco81I        214  CCTTAGG              CC^T[ACGT]AGG
             3998  CCTCAGG             
EcoRI        2188  GAATTC               G^AATTC
             2642  GAATTC              
KpnI          198  GGTACC               GGTAC^C
              525  GGTACC              
RcaI         3410  TCATGA               T^CATGA
             5168  TCATGA              
SapI         3926  GCTCTTC              GCTCTTC(1/4)
             4324  GCTCTTC             
SspBI        3817  TGTACA               T^GTACA
             4633  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (  133 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       ApoI        
AspHI       AvaI        AvaII       BalI        BamHI       
BanI        BanII       BbsI        BbvI        BciVI       
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaHI       BsaJI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssHII      BssKI       BssSI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EagI        
EarI        EclXI       Eco52I      EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     KspI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PleI        PpuMI       PstI        PvuII       
RsaI        SacI        SacII       Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmaI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
XhoII       XmaCI       XmaI