##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

AatII       Acc65I      AcyI        AgeI        Aor51HI     
AscI        AseI        AsnI        Asp718I     AvrII       
BbeI        BbrPI       BciVI       BglII       BlnI        
BsaAI       BsaBI       BsaHI       BsaI        BsiEI       
BsiWI       Bsp1407I    BspDI       BsrGI       BssHII      
BstBI       BstEII      BstPI       ClaI        CpoI        
DrdI        EagI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRV       EcoT22I     Hin1I       
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MunI        NarI        NheI        
NotI        NruI        NsiI        NspV        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshBI       
PvuI        RsrII       SacII       SbfI        SfoI        
SfuI        SgrAI       SnaBI       SphI        Sse8387I    
SspBI       XhoI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccII         932  CGCG                 CG^CG
AclI         4789  AACGTT               AA^CGTT
AflII        1441  CTTAAG               C^TTAAG
AhdI         3811  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I      3511  GAACACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        3811  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         671  TGCGCA               TGC^GCA
BamHI        1092  GGATCC               G^GATCC
BcgI         3111  GCAGCTACGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         1441  CTTAAG               C^TTAAG
BssSI        3527  CTCGTG               CACGAG(-5/-1)
Bst1107I     4003  GTATAC               GTA^TAC
BstUI         932  CGCG                 CG^CG
BstXI        1823  CCAGATCCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      4003  GTATAC               GTA^TAC
DraIII       2450  CACCGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     3811  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI         1198  GGCCGGCC             GGCCGG^CC
FspI          671  TGCGCA               TGC^GCA
HaeII        1593  GGCGCT               [GA]GCGC^[CT]
HgaI         5157  GACGC                GACGC(5/10)
MvnI          932  CGCG                 CG^CG
NaeI         1199  GCCGGC               GCC^GGC
NgoMIV       1199  GCCGGC               G^CCGGC
PacI         5397  TTAATTAA             TTAAT^TAA
PshAI        3151  GACATCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     4789  AACGTT               AA^CGTT
SacI         3687  GAGCTC               GAGCT^C
SapI         2139  GAAGAGC              GCTCTTC(1/4)
SexAI         749  ACCTGGT              A^CC[AT]GGT
SfiI          954  GGCCAACCGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          786  CCCGGG               CCC^GGG
SpeI           66  ACTAGT               A^CTAGT
StuI         1926  AGGCCT               AGG^CCT
SwaI         5246  ATTTAAAT             ATTT^AAAT
XmaCI         786  CCCGGG               C^CCGGG
XmaI          786  CCCGGG               C^CCGGG
XmnI         3511  GAACACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AflIII       3211  ACATGT               A^C[GA][CT]GT
             4769  ACATGT              
Alw44I       2285  GTGCAC               G^TGCAC
             4706  GTGCAC              
AocI         1951  CCTGAGG              CC^T[ACGT]AGG
             2510  CCTCAGG             
ApaLI        2285  GTGCAC               G^TGCAC
             4706  GTGCAC              
AspI          196  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1651  GACATGGTC           
AvaI          786  CCCGGG               C^[CT]CG[GA]G
             2129  CTCGGG              
BglI          955  GCCAACCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1514  GCCTCTACGGC         
BsmBI        4323  CGTCTC               CGTCTC(1/5)
             5155  GAGACG              
BsmI         3587  GAATGC               GAATGC(1/-1)
             1477  GCATTC              
BsoBI         786  CCCGGG               C^[CT]CG[GA]G
             2129  CTCGGG              
BspLU11I     3211  ACATGT               A^CATGT
             4769  ACATGT              
BsrBI         630  CCGCTC               CCGCTC(-3/-3)
             1797  CCGCTC              
BsrFI         173  ACCGGC               [GA]^CCGG[CT]
             1199  GCCGGC              
Bsu36I       1951  CCTGAGG              CC^T[ACGT]AGG
             2510  CCTCAGG             
Cfr10I        173  ACCGGC               [GA]^CCGG[CT]
             1199  GCCGGC              
Eco81I       1951  CCTGAGG              CC^T[ACGT]AGG
             2510  CCTCAGG             
EcoRI        3376  GAATTC               G^AATTC
             4016  GAATTC              
FauI          692  CCCGC                CCCGC(4/6)
             2826  GCGGG               
HindIII      1968  AAGCTT               A^AGCTT
             3825  AAGCTT              
PflFI         196  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1651  GACATGGTC           
PflMI        2730  CCAAGGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3814  CCAGGGTCTGG         
PpuMI          92  AGGTCCC              [GA]G^G[AT]CC[CT]
             3762  AGGTCCT             
SalI          470  GTCGAC               G^TCGAC
             3119  GTCGAC              
ScaI          992  AGTACT               AGT^ACT
             4114  AGTACT              
SspI         1266  AATATT               AAT^ATT
             4208  AATATT              
Tth111I       196  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1651  GACATGGTC           
Van91I       2730  CCAAGGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3814  CCAGGGTCTGG         
XbaI         2475  TCTAGA               T^CTAGA
             5068  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  120 enzymes) #
#########################################

AccI        AccIII      AciI        AcsI        AfaI        
AluI        AlwI        AlwNI       ApaI        ApoI        
AspHI       AvaII       BalI        BanI        BanII       
BbsI        BbvCI       BbvI        BclI        BcnI        
BfaI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspEI       BspHI       BspMI       
BsrDI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstYI       Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FbaI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MroI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NcoI        NdeI        NdeII       
NlaIII      NlaIV       NspI        PleI        PstI        
PvuII       RcaI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        XhoII