##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII AclI AocI Aor51HI ApaI
AscI AspI AviII BsiEI BsiWI
Bsp1407I BspDI BsrGI BssHII Bst1107I
BstZ17I Bsu36I ClaI CpoI DraIII
EagI EclXI Eco47III Eco52I Eco81I
EcoRV FseI FspI MfeI MluI
MunI NdeI NheI NotI NruI
PacI PaeR7I PflFI PmeI Psp1406I
PvuI RsrII SalI ScaI SexAI
SfiI SnaBI SpeI SspBI SspI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 3989 GTCTAC GT^[AC][GT]AC
AflII 314 CTTAAG C^TTAAG
AgeI 900 ACCGGT A^CCGGT
Alw44I 1820 GTGCAC G^TGCAC
ApaLI 1820 GTGCAC G^TGCAC
BbeI 4850 GGCGCC GGCGC^C
BbvCI 364 CCTCAGC CCTCAGC(-5/-2)
BciVI 1645 GGATAC GTATCC(6/5)
BfrI 314 CTTAAG C^TTAAG
BspLU11I 6030 ACATGT A^CATGT
BstBI 4154 TTCGAA TT^CGAA
BstEII 5613 GGTGACC G^GT[ACGT]ACC
BstPI 5613 GGTGACC G^GT[ACGT]ACC
DrdI 5994 GACCCAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 5613 GGTGACC G^GT[ACGT]ACC
EcoRI 5675 GAATTC G^AATTC
EcoT22I 4727 ATGCAT ATGCA^T
HpaI 1638 GTTAAC GTT^AAC
KasI 4850 GGCGCC G^GCGCC
KspI 1785 CCGCGG CCGC^GG
NarI 4850 GGCGCC GG^CGCC
NcoI 1798 CCATGG C^CATGG
NsiI 4727 ATGCAT ATGCA^T
NspV 4154 TTCGAA TT^CGAA
PinAI 900 ACCGGT A^CCGGT
PvuII 379 CAGCTG CAG^CTG
SacII 1785 CCGCGG CCGC^GG
SbfI 3644 CCTGCAGG CCTGCA^GG
SfoI 4850 GGCGCC GGC^GCC
SfuI 4154 TTCGAA TT^CGAA
SgrAI 899 CACCGGTG C[GA]^CCGG[CT]G
SmaI 4843 CCCGGG CCC^GGG
SphI 5889 GCATGC GCATG^C
Sse8387I 3644 CCTGCAGG CCTGCA^GG
SwaI 5843 ATTTAAAT ATTT^AAAT
XmaCI 4843 CCCGGG C^CCGGG
XmaI 4843 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 5594 GGTACC G^GTACC
6155 GGTACC
AccIII 274 TCCGGA T^CCGGA
1495 TCCGGA
AhdI 3330 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3981 GACACCCTGTC
AseI 2433 ATTAAT AT^TAAT
3258 ATTAAT
AsnI 2433 ATTAAT AT^TAAT
3258 ATTAAT
Asp700I 995 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4484 GAAGAGGTTC
Asp718I 5594 GGTACC G^GTACC
6155 GGTACC
AspEI 3330 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3981 GACACCCTGTC
BcgI 4449 GCAGCAACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4633 GCAACAACTTCG
BseAI 274 TCCGGA T^CCGGA
1495 TCCGGA
BsmBI 2091 GAGACG CGTCTC(1/5)
2883 GAGACG
BspEI 274 TCCGGA T^CCGGA
1495 TCCGGA
BspMI 3643 ACCTGC ACCTGC(4/8)
4735 ACCTGC
BsrDI 5525 CATTGC GCAATG(2/0)
6145 GCAATG
BssSI 3228 CACGAG CACGAG(-5/-1)
4112 CACGAG
BstAPI 1416 GCAGATAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2072 GCAGCCTTTGC
DraI 5844 TTTAAA TTT^AAA
6063 TTTAAA
Eam1105I 3330 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3981 GACACCCTGTC
HaeII 4850 GGCGCC [GA]GCGC^[CT]
5325 GGCGCT
KpnI 5594 GGTACC GGTAC^C
6155 GGTACC
MroI 274 TCCGGA T^CCGGA
1495 TCCGGA
NaeI 115 GCCGGC GCC^GGC
3945 GCCGGC
NgoMIV 115 GCCGGC G^CCGGC
3945 GCCGGC
PshAI 1482 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
6133 GACAAGTGTC
PshBI 2433 ATTAAT AT^TAAT
3258 ATTAAT
StuI 2544 AGGCCT AGG^CCT
3357 AGGCCT
XmnI 995 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4484 GAAGAGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII AvrII BalI
BamHI BanI BanII BbrPI BbsI
BbvI BclI BcnI BfaI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspHI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PpuMI PstI RcaI
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII