##################################
# NO CUT ENZYMES (   71 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AgeI        
AscI        AspI        AviII       AvrII       BbeI        
BbrPI       BclI        BlnI        BsaAI       BsaWI       
BseAI       BsiWI       BsmI        BspDI       BspEI       
BssHII      Bst1107I    BstEII      BstPI       BstUI       
BstZ17I     ClaI        CpoI        DraIII      DrdI        
EagI        EarI        EclXI       Eco52I      EcoO65I     
EcoRV       EcoT22I     FbaI        FseI        FspI        
HpaI        KasI        Ksp632I     KspI        MluI        
MroI        MvnI        NarI        NotI        NruI        
NsiI        PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PvuI        RsrII       
SacII       SapI        SbfI        SfiI        SfoI        
SgrAI       SnaBI       SpeI        Sse8387I    SwaI        
Tth111I     


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AflII        3568  CTTAAG               C^TTAAG
AhdI         3219  GACATTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        747  GTGCAC               G^TGCAC
Aor51HI      2322  AGCGCT               AGC^GCT
ApaI          211  GGGCCC               GGGCC^C
ApaLI         747  GTGCAC               G^TGCAC
AspEI        3219  GACATTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI         187  GGATCC               G^GATCC
BcgI         2432  GCACCCAACTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         3568  CTTAAG               C^TTAAG
BglII        5347  AGATCT               A^GATCT
BsaBI         433  GATGAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1030  GAGACC               GGTCTC(1/5)
BsiEI        4083  CGGTCG               CG[GA][CT]^CG
BsmBI         564  GAGACG               CGTCTC(1/5)
BspLU11I     3821  ACATGT               A^CATGT
BsrDI          72  CATTGC               GCAATG(2/0)
BsrFI         866  GCCGGC               [GA]^CCGG[CT]
BssSI        2440  CTCGTG               CACGAG(-5/-1)
BstAPI       4275  GCAGAGGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        2750  TTCGAA               TT^CGAA
Cfr10I        866  GCCGGC               [GA]^CCGG[CT]
Eam1105I     3219  GACATTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     2322  AGCGCT               AGC^GCT
MaeII        4990  ACGT                 A^CGT
MamI          433  GATGAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI          866  GCCGGC               GCC^GGC
NcoI         2909  CCATGG               C^CATGG
NgoMIV        866  GCCGGC               G^CCGGC
NheI          993  GCTAGC               G^CTAGC
NspV         2750  TTCGAA               TT^CGAA
PacI         4518  TTAATTAA             TTAAT^TAA
PaeR7I       3326  CTCGAG               C^TCGAG
SalI         1072  GTCGAC               G^TCGAC
SexAI        1117  ACCTGGT              A^CC[AT]GGT
SfuI         2750  TTCGAA               TT^CGAA
SmaI          692  CCCGGG               CCC^GGG
SphI         3657  GCATGC               GCATG^C
XbaI         1974  TCTAGA               T^CTAGA
XhoI         3326  CTCGAG               C^TCGAG
XmaCI         692  CCCGGG               C^CCGGG
XmaI          692  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

Acc65I       1090  GGTACC               G^GTACC
             1213  GGTACC              
AflIII       3821  ACATGT               A^C[GA][CT]GT
             4990  ACGTGT              
AseI         4521  ATTAAT               AT^TAAT
             5338  ATTAAT              
AsnI         4521  ATTAAT               AT^TAAT
             5338  ATTAAT              
Asp718I      1090  GGTACC               G^GTACC
             1213  GGTACC              
AvaI          692  CCCGGG               C^[CT]CG[GA]G
             3326  CTCGAG              
BbvCI         382  CCTCAGC              CCTCAGC(-5/-2)
             1578  CCTCAGC             
BciVI         617  GTATCC               GTATCC(6/5)
             1122  GTATCC              
BglI          204  GCCTGGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1281  GCCCAGGCGGC         
BsoBI         692  CCCGGG               C^[CT]CG[GA]G
             3326  CTCGAG              
BspHI         854  TCATGA               T^CATGA
             2072  TCATGA              
BsrBI         305  CCGCTC               CCGCTC(-3/-3)
             1344  CCGCTC              
EcoRI         784  GAATTC               G^AATTC
             3835  GAATTC              
FauI           57  GCGGG                CCCGC(4/6)
              244  GCGGG               
HaeII        1159  GGCGCT               [GA]GCGC^[CT]
             2322  AGCGCT              
KpnI         1090  GGTACC               GGTAC^C
             1213  GGTACC              
MfeI          548  CAATTG               C^AATTG
             3310  CAATTG              
MunI          548  CAATTG               C^AATTG
             3310  CAATTG              
NdeI         3302  CATATG               CA^TATG
             4062  CATATG              
NspI         3657  GCATGC               [GA]CATG^[CT]
             3821  ACATGT              
PflMI        2517  CCAAGTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2766  CCAAATTCTGG         
PpuMI           4  GGGTCCC              [GA]G^G[AT]CC[CT]
             4808  AGGTCCC             
PshBI        4521  ATTAAT               AT^TAAT
             5338  ATTAAT              
RcaI          854  TCATGA               T^CATGA
             2072  TCATGA              
SspI         5020  AATATT               AAT^ATT
             5148  AATATT              
StuI         1817  AGGCCT               AGG^CCT
             3107  AGGCCT              
Van91I       2517  CCAAGTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2766  CCAAATTCTGG         
XcmI         1634  CCAGGAACTCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2975  CCAGCAACCTCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  117 enzymes) #
#########################################

AccI        AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       AocI        ApoI        
Asp700I     AspHI       AvaII       BalI        BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaHI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
Bsp1407I    BspMI       BsrGI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Bsu36I      
Cac8I       CelII       CfoI        Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EaeI        
Eco81I      EcoNI       EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HinfI       HpaII       HphI        ItaI        
MaeI        MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScaI        ScrFI       SfaNI       SfcI        SmlI        
SspBI       StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       XmnI