##################################
# NO CUT ENZYMES (   65 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        Aor51HI     AscI        Asp718I     AspEI       
AvrII       BbrPI       BfrI        BglII       BlnI        
BsaBI       BsiEI       BsiWI       BspDI       Bst1107I    
BstBI       BstZ17I     ClaI        CpoI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoT22I     
FseI        HindIII     HpaI        KpnI        KspI        
MamI        MfeI        MluI        MunI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PinAI       PmaCI       PmeI        PmlI        
Psp1406I    PvuI        RsrII       SacII       SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SwaI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AccIII       2561  TCCGGA               T^CCGGA
AseI          506  ATTAAT               AT^TAAT
AsnI          506  ATTAAT               AT^TAAT
AviII        5126  TGCGCA               TGC^GCA
BcgI         4400  GCAGTCTGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2694  GGATAC               GTATCC(6/5)
BclI         1069  TGATCA               T^GATCA
BglI         4467  GCCTGGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         1231  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1231  GCTCAGC              GC^T[ACGT]AGC
BsaAI        2920  CACGTA               [CT]AC^GT[GA]
BseAI        2561  TCCGGA               T^CCGGA
BsmBI        5509  GAGACG               CGTCTC(1/5)
BspEI        2561  TCCGGA               T^CCGGA
BspHI         240  TCATGA               T^CATGA
BspLU11I     5586  ACATGT               A^CATGT
BsrBI        2644  GAGCGG               CCGCTC(-3/-3)
BssHII         47  GCGCGC               G^CGCGC
BstAPI       4558  GCACACACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        815  GGTGACC              G^GT[ACGT]ACC
BstPI         815  GGTGACC              G^GT[ACGT]ACC
CelII        1231  GCTCAGC              GC^T[ACGT]AGC
DraIII       5712  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          973  GACAGTCCGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         569  CCTGGGAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       815  GGTGACC              G^GT[ACGT]ACC
EcoRI         547  GAATTC               G^AATTC
FbaI         1069  TGATCA               T^GATCA
FspI         5126  TGCGCA               TGC^GCA
MroI         2561  TCCGGA               T^CCGGA
PshAI        4456  GACAGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         506  ATTAAT               AT^TAAT
RcaI          240  TCATGA               T^CATGA
SalI         2327  GTCGAC               G^TCGAC
SspI          282  AATATT               AAT^ATT


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccI         2327  GTCGAC               GT^[AC][GT]AC
             4260  GTAGAC              
Alw44I        191  GTGCAC               G^TGCAC
             2763  GTGCAC              
ApaLI         191  GTGCAC               G^TGCAC
             2763  GTGCAC              
Asp700I      3502  GAAATCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3607  GAATGTTTTC          
AspI          299  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1214  GACGAGGTC           
BamHI        2243  GGATCC               G^GATCC
             2954  GGATCC              
BbeI         1270  GGCGCC               GGCGC^C
             5766  GGCGCC              
BsaWI         978  TCCGGT               [AT]^CCGG[AT]
             2561  TCCGGA              
BssSI        1856  CACGAG               CACGAG(-5/-1)
             4605  CTCGTG              
DraI         2399  TTTAAA               TTT^AAA
             5181  TTTAAA              
EcoRV        2105  GATATC               GAT^ATC
             5540  GATATC              
KasI         1270  GGCGCC               G^GCGCC
             5766  GGCGCC              
NarI         1270  GGCGCC               GG^CGCC
             5766  GGCGCC              
NcoI         3677  CCATGG               C^CATGG
             4991  CCATGG              
PaeR7I       1046  CTCGAG               C^TCGAG
             2214  CTCGAG              
PflFI         299  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1214  GACGAGGTC           
SfoI         1270  GGCGCC               GGC^GCC
             5766  GGCGCC              
StuI         2122  AGGCCT               AGG^CCT
             5111  AGGCCT              
Tth111I       299  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1214  GACGAGGTC           
XhoI         1046  CTCGAG               C^TCGAG
             2214  CTCGAG              
XmnI         3502  GAAATCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3607  GAATGTTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  137 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       AocI        
ApaI        ApoI        AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvCI       
BbvI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspMI       BsrDI       BsrFI       BsrGI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
BstYI       Bsu36I      Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        Eco81I      EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NciI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        SacI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmaI        
SmlI        SspBI       StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XbaI        XcmI        XhoII       
XmaCI       XmaI