##################################
# NO CUT ENZYMES (   73 enzymes) #
##################################

AccII       AccIII      AgeI        Aor51HI     ApaI        
AscI        AviII       AvrII       BamHI       BbeI        
BcgI        BglI        BlnI        BsaBI       BsaWI       
BseAI       BsiEI       BsiWI       BspDI       BspEI       
BsrFI       BssHII      BssSI       BstBI       BstEII      
BstPI       BstUI       Cfr10I      ClaI        CpoI        
EagI        EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     FseI        FspI        HincII      HindII      
HpaI        KasI        KspI        MamI        MluI        
MroI        MvnI        NaeI        NarI        NgoMIV      
NotI        NruI        NspV        PinAI       PpuMI       
PshAI       PvuI        RsrII       SacI        SacII       
SalI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       Sse8387I    
SwaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AatII        1372  GACGTC               GACGT^C
AclI         3331  AACGTT               AA^CGTT
AcyI         1372  GACGTC               G[GA]^CG[CT]C
AhdI          774  GACCATCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         4124  CCTGAGG              CC^T[ACGT]AGG
AspEI         774  GACCATCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         1515  CTCGAG               C^[CT]CG[GA]G
BbrPI        6017  CACGTG               CAC^GTG
BclI         5852  TGATCA               T^GATCA
BcnI         1533  CCCGG                CC^[GC]GG
BlpI         2182  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I     2182  GCTTAGC              GC^T[ACGT]AGC
BsaAI        6017  CACGTG               [CT]AC^GT[GA]
BsaHI        1372  GACGTC               G[GA]^CG[CT]C
BsmBI        4935  CGTCTC               CGTCTC(1/5)
BsoBI        1515  CTCGAG               C^[CT]CG[GA]G
BsrBI        1335  CCGCTC               CCGCTC(-3/-3)
Bst1107I     4000  GTATAC               GTA^TAC
BstAPI       5222  GCAGGGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I      4000  GTATAC               GTA^TAC
Bsu36I       4124  CCTGAGG              CC^T[ACGT]AGG
CelII        2182  GCTTAGC              GC^T[ACGT]AGC
DraII        5224  AGGGCCT              [GA]G^G[ACGT]CC[CT]
DraIII       5059  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      774  GACCATCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       4124  CCTGAGG              CC^T[ACGT]AGG
EcoO109I     5224  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoRI        5412  GAATTC               G^AATTC
EcoRV        2503  GATATC               GAT^ATC
EcoT22I      6341  ATGCAT               ATGCA^T
FauI         1421  CCCGC                CCCGC(4/6)
FbaI         5852  TGATCA               T^GATCA
HaeII        4407  AGCGCC               [GA]GCGC^[CT]
HapII        1534  CCGG                 C^CGG
Hin1I        1372  GACGTC               G[GA]^CG[CT]C
HpaII        1534  CCGG                 C^CGG
MspI         1534  CCGG                 C^CGG
NciI         1533  CCCGG                CC^[GC]GG
NcoI         3266  CCATGG               C^CATGG
NheI         3883  GCTAGC               G^CTAGC
NsiI         6341  ATGCAT               ATGCA^T
PacI         4782  TTAATTAA             TTAAT^TAA
PaeR7I       1515  CTCGAG               C^TCGAG
PmaCI        6017  CACGTG               CAC^GTG
PmeI         1080  GTTTAAAC             GTTT^AAAC
PmlI         6017  CACGTG               CAC^GTG
Psp1406I     3331  AACGTT               AA^CGTT
SapI           95  GCTCTTC              GCTCTTC(1/4)
SpeI          333  ACTAGT               A^CTAGT
XhoI         1515  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI         2903  GTCTAC               GT^[AC][GT]AC
             4000  GTATAC              
AflII        2661  CTTAAG               C^TTAAG
             6183  CTTAAG              
Alw44I       1027  GTGCAC               G^TGCAC
             4708  GTGCAC              
ApaLI        1027  GTGCAC               G^TGCAC
             4708  GTGCAC              
Asp700I      5548  GAATTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             6069  GAATGTCTTC          
AspI         3698  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5467  GACTTGGTC           
AvaII          65  GGTCC                G^G[AT]CC
             2205  GGACC               
BbvCI        4403  CCTCAGC              CCTCAGC(-5/-2)
             2345  GCTGAGG             
BfrI         2661  CTTAAG               C^TTAAG
             6183  CTTAAG              
BglII        1685  AGATCT               A^GATCT
             5202  AGATCT              
BsaI          193  GAGACC               GGTCTC(1/5)
             3033  GAGACC              
BspLU11I     5808  ACATGT               A^CATGT
             6377  ACATGT              
DrdI         1099  GACTGGAGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1351  GACAAGAAGGTC        
HgaI          378  GACGC                GACGC(5/10)
             4934  GCGTC               
HindIII       688  AAGCTT               A^AGCTT
             3992  AAGCTT              
PflFI        3698  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5467  GACTTGGTC           
PflMI        1032  CCACGCATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1398  CCACTATCTGG         
ScaI          739  AGTACT               AGT^ACT
              793  AGTACT              
SphI         4599  GCATGC               GCATG^C
             6058  GCATGC              
StuI          989  AGGCCT               AGG^CCT
             5124  AGGCCT              
Tth111I      3698  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5467  GACTTGGTC           
Van91I       1032  CCACGCATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1398  CCACTATCTGG         
XbaI         2472  TCTAGA               T^CTAGA
             4956  TCTAGA              
XcmI          221  CCAGCAGCAATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             6009  CCAGAACCCACGTGG     
XmnI         5548  GAATTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             6069  GAATGTCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  110 enzymes) #
#########################################

Acc65I      AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        AseI        
AsnI        Asp718I     AspHI       BalI        BanI        
BanII       BbsI        BbvI        BciVI       BfaI        
BmyI        BpmI        BpuAI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    Bsp1407I    BspHI       
BspMI       BsrDI       BsrGI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DsaI        EaeI        EarI        EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HhaI        HinP1I      
HinfI       HphI        ItaI        KpnI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MfeI        MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MunI        MvaI        
MwoI        NdeI        NdeII       NlaIII      NlaIV       
NspI        PleI        PshBI       PstI        PvuII       
RcaI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspBI       SspI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII