##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccII AccIII AclI AgeI
AscI AspI AviII BamHI BbeI
BcnI BseAI BsiWI BspEI BssHII
BssSI Bst1107I BstBI BstUI BstZ17I
CpoI EagI EclXI Eco52I EcoRV
FseI FspI HindIII KasI KspI
MfeI MluI MroI MunI MvnI
NarI NciI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI Psp1406I RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 5629 GGTACC G^GTACC
AcyI 934 GACGCC G[GA]^CG[CT]C
Alw44I 1538 GTGCAC G^TGCAC
Aor51HI 5555 AGCGCT AGC^GCT
ApaLI 1538 GTGCAC G^TGCAC
Asp718I 5629 GGTACC G^GTACC
BbrPI 3339 CACGTG CAC^GTG
BciVI 5028 GGATAC GTATCC(6/5)
BclI 2252 TGATCA T^GATCA
BsaAI 3339 CACGTG [CT]AC^GT[GA]
BsaHI 934 GACGCC G[GA]^CG[CT]C
BsaWI 5915 TCCGGT [AT]^CCGG[AT]
BsiEI 1370 CGATCG CG[GA][CT]^CG
BsmBI 2627 GAGACG CGTCTC(1/5)
BsmI 3576 GCATTC GAATGC(1/-1)
BspDI 1652 ATCGAT AT^CGAT
BspLU11I 4616 ACATGT A^CATGT
BstEII 167 GGTGACC G^GT[ACGT]ACC
BstPI 167 GGTGACC G^GT[ACGT]ACC
ClaI 1652 ATCGAT AT^CGAT
DrdI 6024 GACAATAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 5555 AGCGCT AGC^GCT
EcoO65I 167 GGTGACC G^GT[ACGT]ACC
EcoRI 824 GAATTC G^AATTC
EcoT22I 3883 ATGCAT ATGCA^T
FbaI 2252 TGATCA T^GATCA
HaeII 5555 AGCGCT [GA]GCGC^[CT]
Hin1I 934 GACGCC G[GA]^CG[CT]C
HpaI 5358 GTTAAC GTT^AAC
KpnI 5629 GGTACC GGTAC^C
NdeI 4374 CATATG CA^TATG
NheI 67 GCTAGC G^CTAGC
NsiI 3883 ATGCAT ATGCA^T
PmaCI 3339 CACGTG CAC^GTG
PmlI 3339 CACGTG CAC^GTG
PvuI 1370 CGATCG CGAT^CG
SexAI 333 ACCTGGT A^CC[AT]GGT
SphI 3881 GCATGC GCATG^C
XbaI 5722 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 977 GTAGAC GT^[AC][GT]AC
1469 GTCTAC
AflIII 3338 ACACGT A^C[GA][CT]GT
4616 ACATGT
AhdI 750 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3394 GACAGTGGGTC
AseI 4004 ATTAAT AT^TAAT
5868 ATTAAT
AsnI 4004 ATTAAT AT^TAAT
5868 ATTAAT
AspEI 750 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3394 GACAGTGGGTC
AvrII 1025 CCTAGG C^CTAGG
3384 CCTAGG
BglII 387 AGATCT A^GATCT
1184 AGATCT
BlnI 1025 CCTAGG C^CTAGG
3384 CCTAGG
BsaBI 2292 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4126 GATGGCCATC
Bsp1407I 724 TGTACA T^GTACA
921 TGTACA
BspHI 112 TCATGA T^CATGA
705 TCATGA
BsrBI 460 CCGCTC CCGCTC(-3/-3)
163 GAGCGG
BsrDI 744 GCAATG GCAATG(2/0)
1226 GCAATG
BsrGI 724 TGTACA T^GTACA
921 TGTACA
BstAPI 653 GCAGAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
662 GCAAGGAGTGC
DraI 5814 TTTAAA TTT^AAA
5895 TTTAAA
DraIII 946 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1281 CACAGCGTG
Eam1105I 750 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3394 GACAGTGGGTC
MamI 2292 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4126 GATGGCCATC
NaeI 810 GCCGGC GCC^GGC
3530 GCCGGC
NgoMIV 810 GCCGGC G^CCGGC
3530 GCCGGC
PshBI 4004 ATTAAT AT^TAAT
5868 ATTAAT
RcaI 112 TCATGA T^CATGA
705 TCATGA
ScaI 1731 AGTACT AGT^ACT
4172 AGTACT
SspBI 724 TGTACA T^GTACA
921 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI AlwNI AocI ApaI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcgI BfaI BfrI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI