##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccI AclI AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI Asp718I AspEI AspI AviII
BamHI BbrPI BcgI BciVI BclI
BglII BlpI Bpu1102I BsaAI BsaBI
BsaI BsgI BsiWI BsmI Bsp1407I
BspDI BspLU11I BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraIII DrdI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI FbaI FseI FspI KpnI
MamI MluI NcoI NdeI NheI
NotI NspV PaeR7I PflFI PinAI
PmaCI PmeI PmlI Psp1406I RsrII
SacI SalI SapI SbfI SexAI
SfiI SfuI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 28 TCCGGA T^CCGGA
AflII 2972 CTTAAG C^TTAAG
AflIII 825 ACACGT A^C[GA][CT]GT
AlwNI 673 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AscI 737 GGCGCGCC GG^CGCGCC
AspHI 1020 GTGCTC G[AT]GC[AT]^C
AvrII 2870 CCTAGG C^CTAGG
BalI 277 TGGCCA TGG^CCA
BbvCI 1277 GCTGAGG CCTCAGC(-5/-2)
BfrI 2972 CTTAAG C^TTAAG
BlnI 2870 CCTAGG C^CTAGG
BsaWI 28 TCCGGA [AT]^CCGG[AT]
BseAI 28 TCCGGA T^CCGGA
BsiHKAI 1020 GTGCTC G[AT]GC[AT]^C
BsmBI 188 GAGACG CGTCTC(1/5)
BspEI 28 TCCGGA T^CCGGA
BspMI 864 ACCTGC ACCTGC(4/8)
BsrBI 266 CCGCTC CCGCTC(-3/-3)
BstAPI 1447 GCAGGGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1224 CCAAATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 2001 GGATCT [GA]^GATC[CT]
DraI 2613 TTTAAA TTT^AAA
EcoRV 2186 GATATC GAT^ATC
EcoT22I 3310 ATGCAT ATGCA^T
HincII 2716 GTTAAC GT[CT]^[GA]AC
HindII 2716 GTTAAC GT[CT]^[GA]AC
HindIII 1239 AAGCTT A^AGCTT
HpaI 2716 GTTAAC GTT^AAC
MfeI 1399 CAATTG C^AATTG
MflI 2001 GGATCT [GA]^GATC[CT]
MluNI 277 TGGCCA TGG^CCA
MroI 28 TCCGGA T^CCGGA
MscI 277 TGGCCA TGG^CCA
MunI 1399 CAATTG C^AATTG
NruI 439 TCGCGA TCG^CGA
NsiI 3310 ATGCAT ATGCA^T
PacI 1637 TTAATTAA TTAAT^TAA
PshAI 2254 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 314 CGATCG CGAT^CG
SmaI 930 CCCGGG CCC^GGG
SphI 3198 GCATGC GCATG^C
StuI 1745 AGGCCT AGG^CCT
XcmI 1015 CCATAGTGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 2001 GGATCT [GA]^GATC[CT]
XmaCI 930 CCCGGG C^CCGGG
XmaI 930 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AseI 1636 ATTAAT AT^TAAT
2510 ATTAAT
AsnI 1636 ATTAAT AT^TAAT
2510 ATTAAT
Asp700I 1561 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2123 GAAGATATTC
BbeI 287 GGCGCC GGCGC^C
716 GGCGCC
BsmAI 188 GAGAC GTCTC(1/5)
2295 GAGAC
BspHI 1326 TCATGA T^CATGA
1702 TCATGA
BsrDI 1780 CATTGC GCAATG(2/0)
1367 GCAATG
EcoT14I 1765 CCAAGG C^C[AT][AT]GG
2870 CCTAGG
KasI 287 GGCGCC G^GCGCC
716 GGCGCC
MaeII 827 ACGT A^CGT
1246 ACGT
NaeI 284 GCCGGC GCC^GGC
473 GCCGGC
NarI 287 GGCGCC GG^CGCC
716 GGCGCC
NgoMIV 284 GCCGGC G^CCGGC
473 GCCGGC
NspI 1736 ACATGC [GA]CATG^[CT]
3198 GCATGC
PshBI 1636 ATTAAT AT^TAAT
2510 ATTAAT
PstI 679 CTGCAG CTGCA^G
2135 CTGCAG
PvuII 239 CAGCTG CAG^CTG
676 CAGCTG
RcaI 1326 TCATGA T^CATGA
1702 TCATGA
ScaI 1789 AGTACT AGT^ACT
2728 AGTACT
SfoI 287 GGCGCC GGC^GCC
716 GGCGCC
SgrAI 472 CGCCGGCG C[GA]^CCGG[CT]G
882 CGCCGGTG
SspI 1355 AATATT AAT^ATT
1899 AATATT
StyI 1765 CCAAGG C^C[AT][AT]GG
2870 CCTAGG
XmnI 1561 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2123 GAAGATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsiEI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I