##################################
# NO CUT ENZYMES (   89 enzymes) #
##################################

AatII       Acc65I      AccIII      AcyI        AgeI        
AocI        Aor51HI     ApaI        AscI        Asp718I     
AviII       AvrII       BbeI        BbrPI       BglI        
BlnI        BsaAI       BsaHI       BseAI       BsiEI       
BsiWI       BspDI       BspEI       BspHI       BsrBI       
BsrFI       BssHII      BssSI       BstAPI      BstBI       
BstEII      BstPI       Bsu36I      Cfr10I      ClaI        
CpoI        EagI        EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     FseI        FspI        HaeII       
HgaI        Hin1I       HincII      HindII      HpaI        
KasI        KpnI        KspI        MfeI        MluI        
MroI        MunI        NaeI        NarI        NgoMIV      
NotI        NruI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
PvuI        RcaI        RsrII       SacII       SalI        
SbfI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    StuI        
SwaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccII        3133  CGCG                 CG^CG
AclI         1005  AACGTT               AA^CGTT
AflII        2986  CTTAAG               C^TTAAG
AflIII       1039  ACATGT               A^C[GA][CT]GT
AhdI         1760  GACTCTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI        1760  GACTCTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         5694  CTCGGG               C^[CT]CG[GA]G
BalI         5846  TGGCCA               TGG^CCA
BanI         3065  GGTGCC               G^G[CT][GA]CC
BciVI        5330  GGATAC               GTATCC(6/5)
BcnI         3118  CCGGG                CC^[GC]GG
BfrI         2986  CTTAAG               C^TTAAG
BsaWI        3046  ACCGGA               [AT]^CCGG[AT]
BsmBI          17  GAGACG               CGTCTC(1/5)
BsmI         3876  GAATGC               GAATGC(1/-1)
BsoBI        5694  CTCGGG               C^[CT]CG[GA]G
BspLU11I     1039  ACATGT               A^CATGT
BstUI        3133  CGCG                 CG^CG
BstXI        5818  CCAGTAGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII       4452  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         4311  GACATTATAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         4293  CCATGG               C^C[GA][CT]GG
EaeI         5846  TGGCCA               [CT]^GGCC[GA]
Eam1105I     1760  GACTCTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV        2298  GATATC               GAT^ATC
EcoT22I      4254  ATGCAT               ATGCA^T
FauI          118  GCGGG                CCCGC(4/6)
MluNI        5846  TGGCCA               TGG^CCA
MscI         5846  TGGCCA               TGG^CCA
MvnI         3133  CGCG                 CG^CG
NciI         3118  CCGGG                CC^[GC]GG
NcoI         4293  CCATGG               C^CATGG
NheI         3799  GCTAGC               G^CTAGC
NsiI         4254  ATGCAT               ATGCA^T
Psp1406I     1005  AACGTT               AA^CGTT
SapI         3180  GCTCTTC              GCTCTTC(1/4)
ScaI         3622  AGTACT               AGT^ACT
SexAI        4048  ACCTGGT              A^CC[AT]GGT


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

Alw44I       4828  GTGCAC               G^TGCAC
             5487  GTGCAC              
AlwNI         918  CAGCTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             5968  CAGCTGCTG           
ApaLI        4828  GTGCAC               G^TGCAC
             5487  GTGCAC              
BamHI        1016  GGATCC               G^GATCC
             3505  GGATCC              
BbvCI         915  CCTCAGC              CCTCAGC(-5/-2)
             3500  GCTGAGG             
BcgI         2297  CGATATCAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3899  GCAAATAAATCG        
BglII         962  AGATCT               A^GATCT
             1441  AGATCT              
BlpI          859  GCTTAGC              GC^T[ACGT]AGC
             2884  GCTTAGC             
Bpu1102I      859  GCTTAGC              GC^T[ACGT]AGC
             2884  GCTTAGC             
BsaBI        1071  GATCAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1547  GATATGAATC          
BsgI          532  CTGCAC               GTGCAG(16/14)
             4642  CTGCAC              
BsrDI        5168  CATTGC               GCAATG(2/0)
             1881  GCAATG              
Bst1107I      453  GTATAC               GTA^TAC
             1324  GTATAC              
BstZ17I       453  GTATAC               GTA^TAC
             1324  GTATAC              
CelII         859  GCTTAGC              GC^T[ACGT]AGC
             2884  GCTTAGC             
DraI         4520  TTTAAA               TTT^AAA
             5128  TTTAAA              
EcoNI        1402  CCTAGAACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3724  CCTGGTTCAGG         
EcoRI        1257  GAATTC               G^AATTC
             2070  GAATTC              
MamI         1071  GATCAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1547  GATATGAATC          
NspI          148  ACATGC               [GA]CATG^[CT]
             1039  ACATGT              
PflMI         865  CCAAGATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3056  CCAGTATTTGG         
Van91I        865  CCAAGATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3056  CCAGTATTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  109 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        ApoI        AseI        AsnI        Asp700I     
AspHI       AspI        AvaII       BanII       BbsI        
BbvI        BclI        BfaI        BmyI        BpmI        
BpuAI       BsaI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
Bsp1407I    BspMI       BsrGI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EarI        
EcoO109I    EcoRII      EcoT14I     FbaI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NdeI        
NdeII       NlaIII      NlaIV       PflFI       PleI        
PpuMI       PshBI       PstI        PvuII       RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SpeI        SspBI       SspI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       Tth111I     TthHB8I     
XbaI        XcmI        XhoII       XmnI