##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
Acc65I AccIII AflIII AgeI AhdI
Aor51HI AscI Asp718I AspEI BbvCI
BcgI BclI BseAI BsiWI BspEI
BspLU11I BssHII BstXI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV FbaI
FseI HindIII HpaI KpnI MluI
MroI NheI NotI NruI PacI
PaeR7I PinAI PmeI PshAI PvuI
SacI SbfI SexAI SfiI SgrAI
SnaBI SpeI Sse8387I SwaI XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 1801 GACGTC GACGT^C
AclI 4563 AACGTT AA^CGTT
AflII 1108 CTTAAG C^TTAAG
Alw44I 1794 GTGCAC G^TGCAC
ApaI 136 GGGCCC GGGCC^C
ApaLI 1794 GTGCAC G^TGCAC
AseI 4834 ATTAAT AT^TAAT
AsnI 4834 ATTAAT AT^TAAT
AviII 4591 TGCGCA TGC^GCA
AvrII 4414 CCTAGG C^CTAGG
BbeI 1563 GGCGCC GGCGC^C
BbrPI 4438 CACGTG CAC^GTG
BfrI 1108 CTTAAG C^TTAAG
BglI 760 GCCTACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4414 CCTAGG C^CTAGG
BlpI 873 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 873 GCTGAGC GC^T[ACGT]AGC
BsiEI 1699 CGGTCG CG[GA][CT]^CG
BsmBI 249 CGTCTC CGTCTC(1/5)
BspHI 746 TCATGA T^CATGA
BsrBI 1705 CCGCTC CCGCTC(-3/-3)
BssSI 271 CTCGTG CACGAG(-5/-1)
Bst1107I 3385 GTATAC GTA^TAC
BstZ17I 3385 GTATAC GTA^TAC
CelII 873 GCTGAGC GC^T[ACGT]AGC
CpoI 86 CGGTCCG CG^G[AT]CCG
EcoNI 4449 CCTGGATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 4591 TGCGCA TGC^GCA
KasI 1563 GGCGCC G^GCGCC
MfeI 5109 CAATTG C^AATTG
MunI 5109 CAATTG C^AATTG
NarI 1563 GGCGCC GG^CGCC
NdeI 5479 CATATG CA^TATG
PmaCI 4438 CACGTG CAC^GTG
PmlI 4438 CACGTG CAC^GTG
PshBI 4834 ATTAAT AT^TAAT
Psp1406I 4563 AACGTT AA^CGTT
PvuII 1888 CAGCTG CAG^CTG
RcaI 746 TCATGA T^CATGA
RsrII 86 CGGTCCG CG^G[AT]CCG
SalI 286 GTCGAC G^TCGAC
SapI 3022 GAAGAGC GCTCTTC(1/4)
ScaI 626 AGTACT AGT^ACT
SfoI 1563 GGCGCC GGC^GCC
SmaI 1951 CCCGGG CCC^GGG
XmaCI 1951 CCCGGG C^CCGGG
XmaI 1951 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AocI 820 CCTGAGG CC^T[ACGT]AGG
4622 CCTGAGG
Asp700I 1171 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3308 GAACAGGTTC
BamHI 480 GGATCC G^GATCC
2356 GGATCC
BsaAI 1738 TACGTG [CT]AC^GT[GA]
4438 CACGTG
BsaI 43 GAGACC GGTCTC(1/5)
5596 GGTCTC
BsaWI 1088 TCCGGT [AT]^CCGG[AT]
1095 ACCGGA
BsmI 340 GAATGC GAATGC(1/-1)
5416 GAATGC
BspDI 1471 ATCGAT AT^CGAT
4351 ATCGAT
BstBI 1168 TTCGAA TT^CGAA
2091 TTCGAA
Bsu36I 820 CCTGAGG CC^T[ACGT]AGG
4622 CCTGAGG
ClaI 1471 ATCGAT AT^CGAT
4351 ATCGAT
Eco81I 820 CCTGAGG CC^T[ACGT]AGG
4622 CCTGAGG
EcoRI 2958 GAATTC G^AATTC
3967 GAATTC
HgaI 33 GACGC GACGC(5/10)
5692 GACGC
NaeI 121 GCCGGC GCC^GGC
732 GCCGGC
NcoI 104 CCATGG C^CATGG
554 CCATGG
NgoMIV 121 GCCGGC G^CCGGC
732 GCCGGC
NspV 1168 TTCGAA TT^CGAA
2091 TTCGAA
PpuMI 545 GGGACCT [GA]G^G[AT]CC[CT]
1496 GGGACCT
SfuI 1168 TTCGAA TT^CGAA
2091 TTCGAA
StuI 771 AGGCCT AGG^CCT
5084 AGGCCT
XbaI 2503 TCTAGA T^CTAGA
5019 TCTAGA
XmnI 1171 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3308 GAACAGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
AspHI AspI AvaI AvaII BalI
BanI BanII BbsI BbvI BciVI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaBI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrDI BsrFI BsrGI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DraIII DrdI DsaI EaeI EarI
EcoO109I EcoO65I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PflFI PflMI PleI PstI RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XhoII