##################################
# NO CUT ENZYMES (   64 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        Alw44I      AlwNI       ApaLI       
AscI        Asp718I     AviII       BbeI        BciVI       
BfrI        BsaHI       BseAI       BsiEI       BsiWI       
BspDI       BspEI       BspHI       BsrBI       BssHII      
BstAPI      ClaI        CpoI        DraI        EagI        
EclXI       Eco52I      EcoNI       EcoT22I     FseI        
FspI        Hin1I       HpaI        KasI        KpnI        
KspI        MroI        NarI        NheI        NotI        
NruI        NsiI        PacI        PinAI       PmeI        
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SapI        SfiI        SfoI        SgrAI       
SnaBI       SpeI        SphI        SwaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccII        2618  CGCG                 CG^CG
AhdI         2209  GACCCCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      3401  AGCGCT               AGC^GCT
ApaI         3021  GGGCCC               GGGCC^C
AseI         4490  ATTAAT               AT^TAAT
AsnI         4490  ATTAAT               AT^TAAT
AspEI        2209  GACCCCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI        3652  CACGTG               CAC^GTG
BcgI         1164  CGATGACTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI         3440  GCCATGTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1844  AGATCT               A^GATCT
Bsp1407I     5280  TGTACA               T^GTACA
BspLU11I     4483  ACATGT               A^CATGT
BsrGI        5280  TGTACA               T^GTACA
BssSI        2542  CACGAG               CACGAG(-5/-1)
Bst1107I      906  GTATAC               GTA^TAC
BstEII       3931  GGTAACC              G^GT[ACGT]ACC
BstPI        3931  GGTAACC              G^GT[ACGT]ACC
BstUI        2618  CGCG                 CG^CG
BstZ17I       906  GTATAC               GTA^TAC
DraIII       2011  CACCGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         4836  GACTCTAGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     2209  GACCCCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     3401  AGCGCT               AGC^GCT
EcoO65I      3931  GGTAACC              G^GT[ACGT]ACC
EcoRI        3253  GAATTC               G^AATTC
HincII       2557  GTCAAC               GT[CT]^[GA]AC
HindII       2557  GTCAAC               GT[CT]^[GA]AC
MfeI         1597  CAATTG               C^AATTG
MluI         2617  ACGCGT               A^CGCGT
MunI         1597  CAATTG               C^AATTG
MvnI         2618  CGCG                 CG^CG
NaeI           90  GCCGGC               GCC^GGC
NdeI         4896  CATATG               CA^TATG
NgoMIV         90  GCCGGC               G^CCGGC
PflMI         880  CCAACAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        3652  CACGTG               CAC^GTG
PmlI         3652  CACGTG               CAC^GTG
PshBI        4490  ATTAAT               AT^TAAT
SbfI          579  CCTGCAGG             CCTGCA^GG
ScaI         2098  AGTACT               AGT^ACT
SexAI        1500  ACCAGGT              A^CC[AT]GGT
SmaI         4946  CCCGGG               CCC^GGG
Sse8387I      579  CCTGCAGG             CCTGCA^GG
SspBI        5280  TGTACA               T^GTACA
SspI         5275  AATATT               AAT^ATT
Van91I        880  CCAACAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI        4946  CCCGGG               C^CCGGG
XmaI         4946  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccI          906  GTATAC               GT^[AC][GT]AC
             2221  GTAGAC              
Asp700I      2096  GAAGTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4592  GAAGTAGTTC          
AspI          849  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2344  GACTGTGTC           
AvrII        3168  CCTAGG               C^CTAGG
             4755  CCTAGG              
BamHI         575  GGATCC               G^GATCC
             2799  GGATCC              
BbvCI        1249  GCTGAGG              CCTCAGC(-5/-2)
             1729  GCTGAGG             
BlnI         3168  CCTAGG               C^CTAGG
             4755  CCTAGG              
BlpI          764  GCTCAGC              GC^T[ACGT]AGC
             3867  GCTCAGC             
Bpu1102I      764  GCTCAGC              GC^T[ACGT]AGC
             3867  GCTCAGC             
BsaAI        3365  CACGTA               [CT]AC^GT[GA]
             3652  CACGTG              
BsaBI        2766  GATTGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4951  GATGGCTATC          
BsaWI         572  ACCGGA               [AT]^CCGG[AT]
             1586  ACCGGA              
BsrFI          90  GCCGGC               [GA]^CCGG[CT]
             3119  ACCGGC              
CelII         764  GCTCAGC              GC^T[ACGT]AGC
             3867  GCTCAGC             
Cfr10I         90  GCCGGC               [GA]^CCGG[CT]
             3119  ACCGGC              
MamI         2766  GATTGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4951  GATGGCTATC          
PaeR7I         23  CTCGAG               C^TCGAG
             3102  CTCGAG              
PflFI         849  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2344  GACTGTGTC           
PshAI         378  GACTGTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             4913  GACTCTTGTC          
Tth111I       849  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2344  GACTGTGTC           
XbaI         1911  TCTAGA               T^CTAGA
             5155  TCTAGA              
XhoI           23  CTCGAG               C^TCGAG
             3102  CTCGAG              
XmnI         2096  GAAGTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4592  GAAGTAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  122 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AocI        ApoI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbsI        
BbvI        BclI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspMI       BsrDI       BsrI        BssKI       BstBI       
BstF5I      BstNI       BstXI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        Eco81I      
EcoO109I    EcoRII      EcoRV       EcoT14I     FauI        
FbaI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HindIII     
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        NspV        PleI        PpuMI       PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfuI        SmlI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        XhoII