##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AccII AclI AflII AgeI
AhdI Aor51HI ApaI AscI Asp718I
AspEI AviII AvrII BbeI BbrPI
BfrI BlnI BlpI Bpu1102I BsiEI
BsiWI BspDI BssHII BssSI BstAPI
BstUI CelII CfoI ClaI CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoNI FseI FspI HaeII
HhaI HinP1I HpaI KasI KpnI
KspI MluI MvnI NarI NdeI
NheI NotI NruI PaeR7I PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfoI SgrAI SmaI SpeI Sse8387I
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 3584 GACGTC GACGT^C
AccIII 4921 TCCGGA T^CCGGA
AspI 3945 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2589 TGGCCA TGG^CCA
BcgI 8 CGACTTGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4924 GGATAC GTATCC(6/5)
BclI 5032 TGATCA T^GATCA
BcnI 526 CCGGG CC^[GC]GG
BglI 547 GCCCATTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 4921 TCCGGA T^CCGGA
Bsp1407I 2760 TGTACA T^GTACA
BspEI 4921 TCCGGA T^CCGGA
BspHI 3026 TCATGA T^CATGA
BsrBI 1840 CCGCTC CCGCTC(-3/-3)
BsrFI 1083 GCCGGC [GA]^CCGG[CT]
BsrGI 2760 TGTACA T^GTACA
Bst1107I 479 GTATAC GTA^TAC
BstBI 438 TTCGAA TT^CGAA
BstZ17I 479 GTATAC GTA^TAC
Cfr10I 1083 GCCGGC [GA]^CCGG[CT]
DrdI 4977 GACCAACAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1286 CCATGG C^C[GA][CT]GG
EaeI 2589 TGGCCA [CT]^GGCC[GA]
EcoRI 5686 GAATTC G^AATTC
EcoRV 1388 GATATC GAT^ATC
FbaI 5032 TGATCA T^GATCA
MfeI 5692 CAATTG C^AATTG
MluNI 2589 TGGCCA TGG^CCA
MroI 4921 TCCGGA T^CCGGA
MscI 2589 TGGCCA TGG^CCA
MunI 5692 CAATTG C^AATTG
NaeI 1083 GCCGGC GCC^GGC
NciI 526 CCGGG CC^[GC]GG
NcoI 1286 CCATGG C^CATGG
NgoMIV 1083 GCCGGC G^CCGGC
NspV 438 TTCGAA TT^CGAA
PacI 3653 TTAATTAA TTAAT^TAA
PflFI 3945 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 3986 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1812 GACCTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3026 TCATGA T^CATGA
SacI 2843 GAGCTC GAGCT^C
SapI 3735 GCTCTTC GCTCTTC(1/4)
ScaI 3005 AGTACT AGT^ACT
SfuI 438 TTCGAA TT^CGAA
SmlI 1063 CTTGAG C^T[CT][GA]AG
SnaBI 5303 TACGTA TAC^GTA
SspBI 2760 TGTACA T^GTACA
StuI 3402 AGGCCT AGG^CCT
Tth111I 3945 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 479 GTATAC GT^[AC][GT]AC
1819 GTCTAC
AcyI 2293 GGCGTC G[GA]^CG[CT]C
3584 GACGTC
Alw44I 2783 GTGCAC G^TGCAC
4227 GTGCAC
AlwNI 2177 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
3965 CAGTATCTG
AocI 1365 CCTTAGG CC^T[ACGT]AGG
1637 CCTGAGG
ApaLI 2783 GTGCAC G^TGCAC
4227 GTGCAC
AvaI 31 CCCGAG C^[CT]CG[GA]G
62 CCCGAG
BbvCI 1217 CCTCAGC CCTCAGC(-5/-2)
2366 CCTCAGC
BglII 4214 AGATCT A^GATCT
4448 AGATCT
BsaAI 4771 CACGTA [CT]AC^GT[GA]
5303 TACGTA
BsaBI 1310 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4145 GATTCAGATC
BsaHI 2293 GGCGTC G[GA]^CG[CT]C
3584 GACGTC
BsoBI 31 CCCGAG C^[CT]CG[GA]G
62 CCCGAG
BsrDI 1560 GCAATG GCAATG(2/0)
1673 GCAATG
BstEII 1099 GGTGACC G^GT[ACGT]ACC
4576 GGTAACC
BstPI 1099 GGTGACC G^GT[ACGT]ACC
4576 GGTAACC
BstXI 2592 CCAAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3948 CCAGTCAGATGG
Bsu36I 1365 CCTTAGG CC^T[ACGT]AGG
1637 CCTGAGG
DraII 2551 GGGGCCT [GA]G^G[ACGT]CC[CT]
3986 AGGTCCC
Eco81I 1365 CCTTAGG CC^T[ACGT]AGG
1637 CCTGAGG
EcoO109I 2551 GGGGCCT [GA]G^G[ACGT]CC[CT]
3986 AGGTCCC
EcoO65I 1099 GGTGACC G^GT[ACGT]ACC
4576 GGTAACC
EcoT22I 1191 ATGCAT ATGCA^T
2675 ATGCAT
FauI 2269 CCCGC CCCGC(4/6)
2316 GCGGG
Hin1I 2293 GGCGTC G[GA]^CG[CT]C
3584 GACGTC
MamI 1310 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4145 GATTCAGATC
NsiI 1191 ATGCAT ATGCA^T
2675 ATGCAT
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI Asp700I
AspHI AvaII BamHI BanI BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI Bsp1286I BspLU11I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PflMI
PleI PshBI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SphI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XcmI
XhoII XmnI