##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
Acc65I AflII AhdI AscI Asp718I
AspEI AspI AviII BbeI BcgI
BfrI BglII BsiWI BssHII BstBI
DrdI Eam1105I EcoNI EcoT22I FseI
FspI HindIII KasI KpnI MfeI
MluI MunI NaeI NarI NcoI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PmeI PshAI
PvuI SalI SapI SbfI SexAI
SfiI SfoI SfuI SmaI SnaBI
Sse8387I SspI SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 4782 GACGTC GACGT^C
AccIII 4386 TCCGGA T^CCGGA
AclI 4142 AACGTT AA^CGTT
Alw44I 3647 GTGCAC G^TGCAC
AocI 3711 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2605 AGCGCT AGC^GCT
ApaI 4863 GGGCCC GGGCC^C
ApaLI 3647 GTGCAC G^TGCAC
AvrII 3675 CCTAGG C^CTAGG
BbvCI 3560 GCTGAGG CCTCAGC(-5/-2)
BglI 468 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3675 CCTAGG C^CTAGG
BlpI 359 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 359 GCTCAGC GC^T[ACGT]AGC
BsaBI 2103 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 4386 TCCGGA T^CCGGA
BsiEI 106 CGGCCG CG[GA][CT]^CG
BspDI 409 ATCGAT AT^CGAT
BspEI 4386 TCCGGA T^CCGGA
BssSI 4028 CACGAG CACGAG(-5/-1)
Bst1107I 2894 GTATAC GTA^TAC
BstAPI 45 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2894 GTATAC GTA^TAC
Bsu36I 3711 CCTGAGG CC^T[ACGT]AGG
CelII 359 GCTCAGC GC^T[ACGT]AGC
ClaI 409 ATCGAT AT^CGAT
CpoI 187 CGGACCG CG^G[AT]CCG
EagI 106 CGGCCG C^GGCCG
EclXI 106 CGGCCG C^GGCCG
Eco47III 2605 AGCGCT AGC^GCT
Eco52I 106 CGGCCG C^GGCCG
Eco81I 3711 CCTGAGG CC^T[ACGT]AGG
EcoRI 2859 GAATTC G^AATTC
KspI 184 CCGCGG CCGC^GG
MamI 2103 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 4386 TCCGGA T^CCGGA
NdeI 5379 CATATG CA^TATG
NheI 3257 GCTAGC G^CTAGC
PpuMI 856 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 4142 AACGTT AA^CGTT
RsrII 187 CGGACCG CG^G[AT]CCG
SacII 184 CCGCGG CCGC^GG
ScaI 2132 AGTACT AGT^ACT
SgrAI 5278 CACCGGTG C[GA]^CCGG[CT]G
SphI 5053 GCATGC GCATG^C
XbaI 4558 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AvaI 691 CTCGGG C^[CT]CG[GA]G
893 CTCGGG
BalI 709 TGGCCA TGG^CCA
1819 TGGCCA
BamHI 4023 GGATCC G^GATCC
4880 GGATCC
BbrPI 2684 CACGTG CAC^GTG
5646 CACGTG
BciVI 1899 GGATAC GTATCC(6/5)
5072 GTATCC
BclI 2006 TGATCA T^GATCA
5551 TGATCA
BsgI 9 CTGCAC GTGCAG(16/14)
3861 GTGCAG
BsoBI 691 CTCGGG C^[CT]CG[GA]G
893 CTCGGG
BspHI 4053 TCATGA T^CATGA
5548 TCATGA
BsrBI 2349 CCGCTC CCGCTC(-3/-3)
252 GAGCGG
BstEII 773 GGTCACC G^GT[ACGT]ACC
3658 GGTTACC
BstPI 773 GGTCACC G^GT[ACGT]ACC
3658 GGTTACC
DraIII 2684 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
5646 CACGTGGTG
EarI 4201 CTCTTC CTCTTC(1/4)
1534 GAAGAG
EcoO65I 773 GGTCACC G^GT[ACGT]ACC
3658 GGTTACC
FbaI 2006 TGATCA T^GATCA
5551 TGATCA
HincII 2827 GTTAAC GT[CT]^[GA]AC
5034 GTTAAC
HindII 2827 GTTAAC GT[CT]^[GA]AC
5034 GTTAAC
HpaI 2827 GTTAAC GTT^AAC
5034 GTTAAC
Ksp632I 4201 CTCTTC CTCTTC(1/4)
1534 GAAGAG
MluNI 709 TGGCCA TGG^CCA
1819 TGGCCA
MscI 709 TGGCCA TGG^CCA
1819 TGGCCA
PflMI 575 CCAACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1968 CCATTCCGTGG
PmaCI 2684 CACGTG CAC^GTG
5646 CACGTG
PmlI 2684 CACGTG CAC^GTG
5646 CACGTG
RcaI 4053 TCATGA T^CATGA
5548 TCATGA
SacI 1247 GAGCTC GAGCT^C
2076 GAGCTC
Van91I 575 CCAACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1968 CCATTCCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AgeI AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
AspHI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI Bsp1286I
Bsp1407I BspLU11I BspMI BsrDI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EcoO109I EcoRII
EcoRV EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PinAI PleI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SpeI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI