##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AgeI Alw44I ApaLI AscI
Asp718I AviII BamHI BbeI BbrPI
BciVI BfrI BsaBI BsaHI BsiEI
BsiWI BspDI BspLU11I BssHII BstAPI
BstBI BstEII BstPI BstUI ClaI
CpoI DrdI EagI EclXI Eco52I
EcoO65I FseI FspI HgaI Hin1I
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MunI
MvnI NaeI NarI NgoMIV NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I Van91I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccIII 76 TCCGGA T^CCGGA
AhdI 3388 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4668 AGCGCT AGC^GCT
ApaI 4533 GGGCCC GGGCC^C
AseI 3212 ATTAAT AT^TAAT
AsnI 3212 ATTAAT AT^TAAT
AspEI 3388 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 498 GTGCTC G[AT]GC[AT]^C
AspI 2058 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4748 CCTAGG C^CTAGG
BanI 5365 GGCACC G^G[CT][GA]CC
BbsI 928 GTCTTC GAAGAC(2/6)
BglI 139 GCCACTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 167 AGATCT A^GATCT
BlnI 4748 CCTAGG C^CTAGG
BlpI 4245 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4245 GCTCAGC GC^T[ACGT]AGC
BpuAI 928 GTCTTC GAAGAC(2/6)
BsaAI 4061 TACGTG [CT]AC^GT[GA]
BsaWI 76 TCCGGA [AT]^CCGG[AT]
BseAI 76 TCCGGA T^CCGGA
BsiHKAI 498 GTGCTC G[AT]GC[AT]^C
Bsp1407I 2376 TGTACA T^GTACA
BspEI 76 TCCGGA T^CCGGA
BspMI 4054 GCAGGT ACCTGC(4/8)
BsrBI 513 GAGCGG CCGCTC(-3/-3)
BsrFI 148 GCCGGT [GA]^CCGG[CT]
BsrGI 2376 TGTACA T^GTACA
BssSI 99 CACGAG CACGAG(-5/-1)
BstXI 2994 CCAGCTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 4245 GCTCAGC GC^T[ACGT]AGC
Cfr10I 148 GCCGGT [GA]^CCGG[CT]
DraIII 4917 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3388 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4668 AGCGCT AGC^GCT
EcoNI 988 CCTTGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 814 GAATTC G^AATTC
EcoRV 639 GATATC GAT^ATC
HindIII 5234 AAGCTT A^AGCTT
MroI 76 TCCGGA T^CCGGA
MspA1I 126 CAGCTG C[AC]G^C[GT]G
NheI 876 GCTAGC G^CTAGC
PflFI 2058 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2927 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 4254 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3212 ATTAAT AT^TAAT
PvuII 126 CAGCTG CAG^CTG
SfiI 138 GGCCACTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 4855 ACTAGT A^CTAGT
SspBI 2376 TGTACA T^GTACA
Tth111I 2058 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AflIII 567 ACACGT A^C[GA][CT]GT
4062 ACGTGT
AocI 855 CCTGAGG CC^T[ACGT]AGG
5276 CCTGAGG
AvaI 3033 CCCGAG C^[CT]CG[GA]G
4948 CTCGGG
BcgI 101 CGAGGCTTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1973 GCAGGAGAATCG
BclI 2479 TGATCA T^GATCA
3284 TGATCA
BsmBI 1896 CGTCTC CGTCTC(1/5)
5322 CGTCTC
BsoBI 3033 CCCGAG C^[CT]CG[GA]G
4948 CTCGGG
Bsu36I 855 CCTGAGG CC^T[ACGT]AGG
5276 CCTGAGG
Eco81I 855 CCTGAGG CC^T[ACGT]AGG
5276 CCTGAGG
FbaI 2479 TGATCA T^GATCA
3284 TGATCA
SmlI 2699 CTTGAG C^T[CT][GA]AG
4896 CTTGAG
StuI 996 AGGCCT AGG^CCT
4492 AGGCCT
SwaI 2742 ATTTAAAT ATTT^AAAT
4030 ATTTAAAT
TaqI 4852 TCGA T^CGA
5291 TCGA
TthHB8I 4852 TCGA T^CGA
5291 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AvaII
BalI BanII BbvCI BbvI BcnI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspHI BsrDI
BsrI BssKI Bst1107I BstF5I BstNI
BstYI BstZ17I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspI MvaI MwoI NciI NcoI
NdeI NdeII NlaIII NlaIV NsiI
NspI PleI PstI RcaI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SphI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII XmnI