##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AgeI AscI AviII BglII BsiEI
BsiWI BsmI BspDI BspLU11I BssHII
BstBI BstEII BstPI ClaI CpoI
DrdI EagI EclXI Eco52I EcoNI
EcoO65I EcoRV EcoT22I FseI FspI
HgaI KspI MfeI MluI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PpuMI PvuI RsrII
SacII SalI SbfI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
StuI SwaI XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 3410 GGTACC G^GTACC
AccIII 2108 TCCGGA T^CCGGA
AclI 4427 AACGTT AA^CGTT
AflII 3615 CTTAAG C^TTAAG
AflIII 4466 ACACGT A^C[GA][CT]GT
Alw44I 1910 GTGCAC G^TGCAC
Aor51HI 1878 AGCGCT AGC^GCT
ApaI 993 GGGCCC GGGCC^C
ApaLI 1910 GTGCAC G^TGCAC
Asp718I 3410 GGTACC G^GTACC
AspI 5715 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3019 CCTAGG C^CTAGG
BamHI 1860 GGATCC G^GATCC
BbrPI 1865 CACGTG CAC^GTG
BciVI 2248 GTATCC GTATCC(6/5)
BfrI 3615 CTTAAG C^TTAAG
BglI 2767 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3019 CCTAGG C^CTAGG
BlpI 2507 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2507 GCTGAGC GC^T[ACGT]AGC
BsaBI 2123 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2108 TCCGGA [AT]^CCGG[AT]
BseAI 2108 TCCGGA T^CCGGA
BsmBI 1234 CGTCTC CGTCTC(1/5)
BspEI 2108 TCCGGA T^CCGGA
BspHI 4831 TCATGA T^CATGA
BsrFI 2463 ACCGGC [GA]^CCGG[CT]
BssSI 2375 CTCGTG CACGAG(-5/-1)
Bst1107I 4470 GTATAC GTA^TAC
BstZ17I 4470 GTATAC GTA^TAC
CelII 2507 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2463 ACCGGC [GA]^CCGG[CT]
DraII 992 GGGGCCC [GA]G^G[ACGT]CC[CT]
Eco47III 1878 AGCGCT AGC^GCT
EcoO109I 992 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 214 GAATTC G^AATTC
KpnI 3410 GGTACC GGTAC^C
MamI 2123 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2108 TCCGGA T^CCGGA
PflFI 5715 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1865 CACGTG CAC^GTG
PmlI 1865 CACGTG CAC^GTG
PshAI 244 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4427 AACGTT AA^CGTT
PstI 687 CTGCAG CTGCA^G
RcaI 4831 TCATGA T^CATGA
SacI 3043 GAGCTC GAGCT^C
SapI 2088 GCTCTTC GCTCTTC(1/4)
Tth111I 5715 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AatII 1232 GACGTC GACGT^C
1404 GACGTC
AhdI 1547 GACCACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1679 GACGAGCTGTC
AocI 3967 CCTGAGG CC^T[ACGT]AGG
5507 CCTGAGG
AspEI 1547 GACCACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1679 GACGAGCTGTC
AvaI 1874 CCCGAG C^[CT]CG[GA]G
5616 CTCGGG
AvaII 3471 GGTCC G^G[AT]CC
4793 GGTCC
BalI 381 TGGCCA TGG^CCA
5105 TGGCCA
BbeI 450 GGCGCC GGCGC^C
1784 GGCGCC
BbvCI 185 GCTGAGG CCTCAGC(-5/-2)
338 GCTGAGG
BcgI 484 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2307 GCATGCACCTCG
BclI 4642 TGATCA T^GATCA
4729 TGATCA
BcnI 1264 CCGGG CC^[GC]GG
1815 CCCGG
BsaAI 1865 CACGTG [CT]AC^GT[GA]
4467 CACGTA
BsoBI 1874 CCCGAG C^[CT]CG[GA]G
5616 CTCGGG
BsrBI 1419 CCGCTC CCGCTC(-3/-3)
146 GAGCGG
BstYI 1860 GGATCC [GA]^GATC[CT]
2542 GGATCT
Bsu36I 3967 CCTGAGG CC^T[ACGT]AGG
5507 CCTGAGG
EaeI 381 TGGCCA [CT]^GGCC[GA]
5105 TGGCCA
Eam1105I 1547 GACCACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1679 GACGAGCTGTC
Eco81I 3967 CCTGAGG CC^T[ACGT]AGG
5507 CCTGAGG
FauI 1418 CCCGC CCCGC(4/6)
447 GCGGG
FbaI 4642 TGATCA T^GATCA
4729 TGATCA
KasI 450 GGCGCC G^GCGCC
1784 GGCGCC
MflI 1860 GGATCC [GA]^GATC[CT]
2542 GGATCT
MluNI 381 TGGCCA TGG^CCA
5105 TGGCCA
MscI 381 TGGCCA TGG^CCA
5105 TGGCCA
NarI 450 GGCGCC GG^CGCC
1784 GGCGCC
NciI 1264 CCGGG CC^[GC]GG
1815 CCCGG
PvuII 293 CAGCTG CAG^CTG
2770 CAGCTG
ScaI 812 AGTACT AGT^ACT
4603 AGTACT
SfoI 450 GGCGCC GGC^GCC
1784 GGCGCC
SphI 2307 GCATGC GCATG^C
2560 GCATGC
XhoII 1860 GGATCC [GA]^GATC[CT]
2542 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
AseI AsnI Asp700I AspHI BanI
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspMI
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraIII DsaI EarI EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PshBI RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XmnI