##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI ApoI AscI
AseI AsnI AviII AvrII BbeI
BbrPI BciVI BclI BfrI BlnI
BsaAI BseAI BsiWI BspDI BspEI
BspHI BspLU11I BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DrdI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KasI KspI MfeI
MluI MroI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 3579 GGTACC G^GTACC
AcyI 468 GACGCC G[GA]^CG[CT]C
AhdI 945 GACAGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4308 GTGCAC G^TGCAC
Aor51HI 293 AGCGCT AGC^GCT
ApaLI 4308 GTGCAC G^TGCAC
Asp700I 1512 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3579 GGTACC G^GTACC
AspEI 945 GACAGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 902 GGATCC G^GATCC
BfaI 3019 CTAG C^TAG
BsaBI 4184 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 468 GACGCC G[GA]^CG[CT]C
BsaWI 4578 ACCGGA [AT]^CCGG[AT]
BsiEI 547 CGGCCG CG[GA][CT]^CG
BsmBI 252 GAGACG CGTCTC(1/5)
Bsp1407I 1093 TGTACA T^GTACA
BspMI 1346 GCAGGT ACCTGC(4/8)
BsrBI 1160 GAGCGG CCGCTC(-3/-3)
BsrFI 2978 GCCGGC [GA]^CCGG[CT]
BsrGI 1093 TGTACA T^GTACA
Cfr10I 2978 GCCGGC [GA]^CCGG[CT]
DraIII 1547 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 547 CGGCCG C^GGCCG
Eam1105I 945 GACAGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 547 CGGCCG C^GGCCG
Eco47III 293 AGCGCT AGC^GCT
Eco52I 547 CGGCCG C^GGCCG
HaeII 293 AGCGCT [GA]GCGC^[CT]
Hin1I 468 GACGCC G[GA]^CG[CT]C
HincII 4542 GTCAAC GT[CT]^[GA]AC
HindII 4542 GTCAAC GT[CT]^[GA]AC
KpnI 3579 GGTACC GGTAC^C
MaeI 3019 CTAG C^TAG
MamI 4184 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 4333 TTAA T^TAA
NaeI 2978 GCCGGC GCC^GGC
NgoMIV 2978 GCCGGC G^CCGGC
NspI 3036 GCATGT [GA]CATG^[CT]
PflMI 1392 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3157 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 2869 ACCAGGT A^CC[AT]GGT
SmaI 4384 CCCGGG CCC^GGG
SmlI 3901 CTCAAG C^T[CT][GA]AG
SspBI 1093 TGTACA T^GTACA
TaqI 766 TCGA T^CGA
Tru9I 4333 TTAA T^TAA
Tsp509I 1270 AATT ^AATT
TthHB8I 766 TCGA T^CGA
Van91I 1392 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 4384 CCCGGG C^CCGGG
XmaI 4384 CCCGGG C^CCGGG
XmnI 1512 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 9 enzymes) #
###################################
AspHI 10 GTGCTC G[AT]GC[AT]^C
4308 GTGCAC
BcgI 1007 CGAGGCTTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1028 CGACACCTCTGC
BsaI 3096 GAGACC GGTCTC(1/5)
4089 GAGACC
BsiHKAI 10 GTGCTC G[AT]GC[AT]^C
4308 GTGCAC
BsmI 2828 GAATGC GAATGC(1/-1)
3983 GCATTC
BsrDI 1449 GCAATG GCAATG(2/0)
4140 GCAATG
BstAPI 2736 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3842 GCAGATCTTGC
HgaI 468 GACGC GACGC(5/10)
3387 GACGC
SfiI 747 GGCCAGGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3311 GGCCACAGAGGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PflFI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TfiI TseI Tsp45I TspRI
Tth111I XcmI XhoII