##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

AatII       AccI        AclI        AgeI        AhdI        
Aor51HI     AscI        AseI        AsnI        AspEI       
BbeI        BbrPI       BcgI        BciVI       BclI        
BglI        BglII       BsaAI       BsaBI       BsiEI       
BsiWI       BspDI       BssHII      BssSI       Bst1107I    
BstBI       BstZ17I     ClaI        CpoI        DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoRV       EcoT22I     FbaI        FseI        HaeII       
HincII      HindII      HindIII     HpaI        KasI        
KspI        MaeII       MamI        MluI        NaeI        
NarI        NgoMIV      NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SnaBI       SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

Acc65I       4374  GGTACC               G^GTACC
AccII         342  CGCG                 CG^CG
AccIII       2411  TCCGGA               T^CCGGA
AflII        4658  CTTAAG               C^TTAAG
Asp718I      4374  GGTACC               G^GTACC
AviII         400  TGCGCA               TGC^GCA
BfrI         4658  CTTAAG               C^TTAAG
BseAI        2411  TCCGGA               T^CCGGA
Bsp1407I     4008  TGTACA               T^GTACA
BspEI        2411  TCCGGA               T^CCGGA
BsrGI        4008  TGTACA               T^GTACA
BstAPI       1729  GCACTCAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         342  CGCG                 CG^CG
BstXI        3706  CCACTCCTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI           53  TTTAAA               TTT^AAA
DraIII       1730  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
FspI          400  TGCGCA               TGC^GCA
KpnI         4374  GGTACC               GGTAC^C
MfeI          187  CAATTG               C^AATTG
MroI         2411  TCCGGA               T^CCGGA
MunI          187  CAATTG               C^AATTG
MvnI          342  CGCG                 CG^CG
NcoI         3195  CCATGG               C^CATGG
NdeI         3154  CATATG               CA^TATG
NheI          361  GCTAGC               G^CTAGC
SbfI         2967  CCTGCAGG             CCTGCA^GG
SpeI         4520  ACTAGT               A^CTAGT
SphI         1922  GCATGC               GCATG^C
Sse8387I     2967  CCTGCAGG             CCTGCA^GG
SspBI        4008  TGTACA               T^GTACA
TaqI          748  TCGA                 T^CGA
TthHB8I       748  TCGA                 T^CGA
XbaI         3761  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcyI           42  GGCGTC               G[GA]^CG[CT]C
             4680  GGCGTC              
AflIII       1566  ACATGT               A^C[GA][CT]GT
             3416  ACATGT              
Alw44I       2306  GTGCAC               G^TGCAC
             3631  GTGCAC              
ApaLI        2306  GTGCAC               G^TGCAC
             3631  GTGCAC              
Asp700I      2373  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4117  GAAAATTTTC          
AspI          217  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1606  GACTATGTC           
AvrII        3870  CCTAGG               C^CTAGG
             4082  CCTAGG              
BamHI         606  GGATCC               G^GATCC
             3907  GGATCC              
BlnI         3870  CCTAGG               C^CTAGG
             4082  CCTAGG              
BsaHI          42  GGCGTC               G[GA]^CG[CT]C
             4680  GGCGTC              
BsaI          215  GAGACC               GGTCTC(1/5)
             4252  GGTCTC              
BsmBI          44  CGTCTC               CGTCTC(1/5)
              210  GAGACG              
BsmI         1240  GCATTC               GAATGC(1/-1)
             3778  GCATTC              
BspHI        1820  TCATGA               T^CATGA
             4204  TCATGA              
BspLU11I     1566  ACATGT               A^CATGT
             3416  ACATGT              
BspMI        3321  ACCTGC               ACCTGC(4/8)
              897  GCAGGT              
BsrBI        3094  CCGCTC               CCGCTC(-3/-3)
             3133  GAGCGG              
FauI          692  GCGGG                CCCGC(4/6)
             3135  GCGGG               
Hin1I          42  GGCGTC               G[GA]^CG[CT]C
             4680  GGCGTC              
PflFI         217  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1606  GACTATGTC           
RcaI         1820  TCATGA               T^CATGA
             4204  TCATGA              
SapI          832  GAAGAGC              GCTCTTC(1/4)
             3096  GCTCTTC             
SexAI         218  ACCAGGT              A^CC[AT]GGT
             3173  ACCAGGT             
SmaI          276  CCCGGG               CCC^GGG
             2342  CCCGGG              
SspI         3208  AATATT               AAT^ATT
             4451  AATATT              
Tth111I       217  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1606  GACTATGTC           
XmaCI         276  CCCGGG               C^CCGGG
             2342  CCCGGG              
XmaI          276  CCCGGG               C^CCGGG
             2342  CCCGGG              
XmnI         2373  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4117  GAAAATTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  118 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       AocI        ApaI        ApoI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbsI        BbvCI       BbvI        BcnI        BfaI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrDI       BsrFI       BsrI        BssKI       
BstEII      BstF5I      BstNI       BstPI       BstYI       
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StuI        StyI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Van91I      XcmI        XhoII