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# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AccIII AgeI AhdI Aor51HI
ApaI AscI AspEI AspI BbeI
BbrPI BbvCI BsaAI BseAI BsiWI
BspDI BspEI BspHI BspMI BssHII
BssSI BstBI BstEII BstPI ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I KasI MluI
MroI NarI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI RcaI RsrII SapI
SbfI SexAI SfiI SfoI SfuI
SnaBI Sse8387I SwaI Tth111I XcmI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 6252 GGTACC G^GTACC
AcyI 267 GGCGTC G[GA]^CG[CT]C
AocI 2588 CCTGAGG CC^T[ACGT]AGG
Asp700I 959 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 6252 GGTACC G^GTACC
AviII 3016 TGCGCA TGC^GCA
BalI 6400 TGGCCA TGG^CCA
BamHI 366 GGATCC G^GATCC
BanI 6252 GGTACC G^G[CT][GA]CC
BcgI 103 GCATCGGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5012 GGATAC GTATCC(6/5)
BglI 156 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3005 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 3005 GCTTAGC GC^T[ACGT]AGC
BsaHI 267 GGCGTC G[GA]^CG[CT]C
BsaWI 5376 ACCGGA [AT]^CCGG[AT]
BsiEI 1372 CGATCG CG[GA][CT]^CG
BsmI 5704 GCATTC GAATGC(1/-1)
BsrBI 830 GAGCGG CCGCTC(-3/-3)
BstXI 4955 CCATCCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2588 CCTGAGG CC^T[ACGT]AGG
CelII 3005 GCTTAGC GC^T[ACGT]AGC
Eco81I 2588 CCTGAGG CC^T[ACGT]AGG
EcoNI 1272 CCTATTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 175 GGCCGGCC GGCCGG^CC
FspI 3016 TGCGCA TGC^GCA
HaeII 6204 GGCGCT [GA]GCGC^[CT]
Hin1I 267 GGCGTC G[GA]^CG[CT]C
HindIII 636 AAGCTT A^AGCTT
KpnI 6252 GGTACC GGTAC^C
MluNI 6400 TGGCCA TGG^CCA
MscI 6400 TGGCCA TGG^CCA
NaeI 176 GCCGGC GCC^GGC
NcoI 387 CCATGG C^CATGG
NgoMIV 176 GCCGGC G^CCGGC
PaeR7I 6533 CTCGAG C^TCGAG
PvuI 1372 CGATCG CGAT^CG
SacI 482 GAGCTC GAGCT^C
SalI 1950 GTCGAC G^TCGAC
SgrAI 263 CACCGGCG C[GA]^CCGG[CT]G
SmaI 198 CCCGGG CCC^GGG
XhoI 6533 CTCGAG C^TCGAG
XmaCI 198 CCCGGG C^CCGGG
XmaI 198 CCCGGG C^CCGGG
XmnI 959 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AclI 1602 AACGTT AA^CGTT
2814 AACGTT
AflII 5583 CTTAAG C^TTAAG
6244 CTTAAG
Alw44I 2052 GTGCAC G^TGCAC
3609 GTGCAC
AlwNI 2343 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
5053 CAGTAACTG
ApaLI 2052 GTGCAC G^TGCAC
3609 GTGCAC
AvrII 3564 CCTAGG C^CTAGG
5948 CCTAGG
BclI 646 TGATCA T^GATCA
2302 TGATCA
BfrI 5583 CTTAAG C^TTAAG
6244 CTTAAG
BglII 1987 AGATCT A^GATCT
4649 AGATCT
BlnI 3564 CCTAGG C^CTAGG
5948 CCTAGG
BpmI 1459 CTCCAG CTGGAG(16/14)
1964 CTCCAG
BsaBI 361 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5555 GATTTTTATC
BsaI 896 GGTCTC GGTCTC(1/5)
1464 GGTCTC
BseRI 25 GAGGAG GAGGAG(10/8)
479 GAGGAG
BsgI 4772 CTGCAC GTGCAG(16/14)
4966 GTGCAG
BsmBI 1406 CGTCTC CGTCTC(1/5)
6352 GAGACG
BspLU11I 2734 ACATGT A^CATGT
3800 ACATGT
BsrFI 176 GCCGGC [GA]^CCGG[CT]
264 ACCGGC
Bst1107I 2603 GTATAC GTA^TAC
3452 GTATAC
BstAPI 2083 GCACATATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2618 GCATAAAATGC
BstZ17I 2603 GTATAC GTA^TAC
3452 GTATAC
Cfr10I 176 GCCGGC [GA]^CCGG[CT]
264 ACCGGC
DrdI 261 GACACCGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5880 GACATAACTGTC
EaeI 178 CGGCCA [CT]^GGCC[GA]
6400 TGGCCA
FbaI 646 TGATCA T^GATCA
2302 TGATCA
KspI 68 CCGCGG CCGC^GG
160 CCGCGG
MamI 361 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5555 GATTTTTATC
MfeI 1092 CAATTG C^AATTG
5661 CAATTG
MunI 1092 CAATTG C^AATTG
5661 CAATTG
NheI 5916 GCTAGC G^CTAGC
6410 GCTAGC
PflMI 5936 CCAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6130 CCAACTTTTGG
Psp1406I 1602 AACGTT AA^CGTT
2814 AACGTT
PstI 954 CTGCAG CTGCA^G
1983 CTGCAG
PvuII 3498 CAGCTG CAG^CTG
3699 CAGCTG
SacII 68 CCGCGG CCGC^GG
160 CCGCGG
ScaI 4002 AGTACT AGT^ACT
4319 AGTACT
SpeI 5443 ACTAGT A^CTAGT
5543 ACTAGT
SphI 3159 GCATGC GCATG^C
5241 GCATGC
StuI 5322 AGGCCT AGG^CCT
5400 AGGCCT
Van91I 5936 CCAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6130 CCAACTTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI ApoI AseI
AsnI AspHI AvaI AvaII BanII
BbsI BbvI BcnI BfaI BmyI
BpuAI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EarI EcoO109I EcoRI EcoRII
EcoRV EcoT14I EcoT22I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeI
NdeII NlaIII NlaIV NsiI NspI
PleI PpuMI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XhoII