##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI AscI Asp718I AspEI AviII
BamHI BbrPI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BstBI BstEII
BstPI ClaI DraIII EagI Eam1105I
EclXI Eco52I EcoO65I EcoRI EcoRV
FseI FspI KpnI MfeI MluI
MroI MunI NaeI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
SacI SapI SbfI SexAI SfiI
SfuI SgrAI SnaBI SphI Sse8387I
SwaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AocI 3508 CCTGAGG CC^T[ACGT]AGG
Aor51HI 4767 AGCGCT AGC^GCT
AvrII 3214 CCTAGG C^CTAGG
BcgI 5267 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2761 GGATAC GTATCC(6/5)
BclI 2202 TGATCA T^GATCA
BglI 3537 GCCAACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3214 CCTAGG C^CTAGG
BsaAI 5115 CACGTA [CT]AC^GT[GA]
BsaBI 49 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspMI 15 GCAGGT ACCTGC(4/8)
BsrBI 1257 CCGCTC CCGCTC(-3/-3)
BsrFI 280 ACCGGC [GA]^CCGG[CT]
BssHII 258 GCGCGC G^CGCGC
BssSI 1857 CACGAG CACGAG(-5/-1)
Bst1107I 3307 GTATAC GTA^TAC
BstZ17I 3307 GTATAC GTA^TAC
Bsu36I 3508 CCTGAGG CC^T[ACGT]AGG
Cfr10I 280 ACCGGC [GA]^CCGG[CT]
CpoI 383 CGGACCG CG^G[AT]CCG
DrdI 3864 GACTCTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4767 AGCGCT AGC^GCT
Eco81I 3508 CCTGAGG CC^T[ACGT]AGG
FbaI 2202 TGATCA T^GATCA
HgaI 216 GCGTC GACGC(5/10)
HpaI 2771 GTTAAC GTT^AAC
KspI 151 CCGCGG CCGC^GG
MamI 49 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PaeR7I 1567 CTCGAG C^TCGAG
PshAI 607 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 383 CGGACCG CG^G[AT]CCG
SacII 151 CCGCGG CCGC^GG
SalI 5419 GTCGAC G^TCGAC
ScaI 2682 AGTACT AGT^ACT
SpeI 4210 ACTAGT A^CTAGT
XcmI 555 CCACCTAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1567 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 168 GGCGCC G[GA]^CG[CT]C
4930 GGCGCC
AflII 4144 CTTAAG C^TTAAG
4735 CTTAAG
Alw44I 1311 GTGCAC G^TGCAC
2367 GTGCAC
AlwNI 3609 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
4587 CAGGGACTG
ApaI 389 GGGCCC GGGCC^C
1703 GGGCCC
ApaLI 1311 GTGCAC G^TGCAC
2367 GTGCAC
BbeI 168 GGCGCC GGCGC^C
4930 GGCGCC
BfrI 4144 CTTAAG C^TTAAG
4735 CTTAAG
BlpI 463 GCTCAGC GC^T[ACGT]AGC
541 GCTGAGC
Bpu1102I 463 GCTCAGC GC^T[ACGT]AGC
541 GCTGAGC
BsaHI 168 GGCGCC G[GA]^CG[CT]C
4930 GGCGCC
BsmBI 4972 GAGACG CGTCTC(1/5)
5348 GAGACG
BspLU11I 2767 ACATGT A^CATGT
3831 ACATGT
BsrDI 4330 CATTGC GCAATG(2/0)
1229 GCAATG
BstAPI 1229 GCAATGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4868 GCAACCTCTGC
CelII 463 GCTCAGC GC^T[ACGT]AGC
541 GCTGAGC
EcoNI 474 CCTGTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4580 CCTAAGTCAGG
Hin1I 168 GGCGCC G[GA]^CG[CT]C
4930 GGCGCC
HincII 2771 GTTAAC GT[CT]^[GA]AC
5419 GTCGAC
HindII 2771 GTTAAC GT[CT]^[GA]AC
5419 GTCGAC
HindIII 3999 AAGCTT A^AGCTT
5747 AAGCTT
KasI 168 GGCGCC G^GCGCC
4930 GGCGCC
NarI 168 GGCGCC GG^CGCC
4930 GGCGCC
PflMI 3608 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6183 CCATTGGGTGG
PstI 4435 CTGCAG CTGCA^G
6411 CTGCAG
SfoI 168 GGCGCC GGC^GCC
4930 GGCGCC
Van91I 3608 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6183 CCATTGGGTGG
XbaI 2310 TCTAGA T^CTAGA
5525 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI ApoI AseI
AsnI Asp700I AspHI AspI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglII
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspHI BsrGI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeI NdeII NlaIII NlaIV NsiI
NspI PflFI PleI PpuMI PshBI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SspBI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XhoII XmaCI
XmaI XmnI