##################################
# NO CUT ENZYMES (   60 enzymes) #
##################################

AatII       Acc65I      AclI        AgeI        AhdI        
ApaI        AscI        Asp718I     AspEI       AvrII       
BalI        BbeI        BclI        BlnI        BsmBI       
BspHI       BsrFI       BssHII      Cfr10I      CpoI        
DraIII      EagI        Eam1105I    EclXI       Eco52I      
FbaI        FseI        KasI        KpnI        KspI        
MluI        MluNI       MscI        NaeI        NarI        
NcoI        NgoMIV      NotI        NruI        PacI        
PinAI       PmeI        PshAI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
SfiI        SfoI        SgrAI       SmaI        SnaBI       
Sse8387I    SwaI        XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccIII        129  TCCGGA               T^CCGGA
AflII         974  CTTAAG               C^TTAAG
AocI         3740  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      5605  AGCGCT               AGC^GCT
AvaI         1587  CTCGAG               C^[CT]CG[GA]G
AviII         541  TGCGCA               TGC^GCA
BamHI         148  GGATCC               G^GATCC
BanI         4688  GGCACC               G^G[CT][GA]CC
BbrPI        2626  CACGTG               CAC^GTG
BbvCI        3188  CCTCAGC              CCTCAGC(-5/-2)
BcgI         1247  CGAACAAACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        3581  GGATAC               GTATCC(6/5)
BfrI          974  CTTAAG               C^TTAAG
BsaAI        2626  CACGTG               [CT]AC^GT[GA]
BsaWI         129  TCCGGA               [AT]^CCGG[AT]
BseAI         129  TCCGGA               T^CCGGA
BsiEI        3399  CGGTCG               CG[GA][CT]^CG
BsiWI        5702  CGTACG               C^GTACG
BsoBI        1587  CTCGAG               C^[CT]CG[GA]G
BspEI         129  TCCGGA               T^CCGGA
BspLU11I     5255  ACATGT               A^CATGT
BsrBI          83  GAGCGG               CCGCTC(-3/-3)
BssSI        3612  CTCGTG               CACGAG(-5/-1)
BstBI        1245  TTCGAA               TT^CGAA
Bsu36I       3740  CCTCAGG              CC^T[ACGT]AGG
DrdI         1630  GACATTACAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI           21  CCACGG               C^C[GA][CT]GG
EaeI           12  CGGCCA               [CT]^GGCC[GA]
Eco47III     5605  AGCGCT               AGC^GCT
Eco81I       3740  CCTCAGG              CC^T[ACGT]AGG
EcoRI         478  GAATTC               G^AATTC
EcoT22I       282  ATGCAT               ATGCA^T
FspI          541  TGCGCA               TGC^GCA
HaeII        5605  AGCGCT               [GA]GCGC^[CT]
MroI          129  TCCGGA               T^CCGGA
NheI         1312  GCTAGC               G^CTAGC
NsiI          282  ATGCAT               ATGCA^T
NspV         1245  TTCGAA               TT^CGAA
PaeR7I       1587  CTCGAG               C^TCGAG
PflMI        2780  CCACCAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        2626  CACGTG               CAC^GTG
PmlI         2626  CACGTG               CAC^GTG
SexAI         749  ACCAGGT              A^CC[AT]GGT
SfuI         1245  TTCGAA               TT^CGAA
StuI         2687  AGGCCT               AGG^CCT
Van91I       2780  CCACCAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         1587  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AcyI           42  GACGCC               G[GA]^CG[CT]C
             2012  GGCGTC              
AflIII       2625  ACACGT               A^C[GA][CT]GT
             5255  ACATGT              
Alw44I       3348  GTGCAC               G^TGCAC
             4863  GTGCAC              
ApaLI        3348  GTGCAC               G^TGCAC
             4863  GTGCAC              
AseI         5410  ATTAAT               AT^TAAT
             5961  ATTAAT              
AsnI         5410  ATTAAT               AT^TAAT
             5961  ATTAAT              
AspI         2525  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3531  GACGCAGTC           
BglI           30  GCCGGGCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             4724  GCCTCTAAGGC         
BlpI         2663  GCTGAGC              GC^T[ACGT]AGC
             4049  GCTCAGC             
Bpu1102I     2663  GCTGAGC              GC^T[ACGT]AGC
             4049  GCTCAGC             
BsaBI         443  GATGAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2576  GATCTAGATC          
BsaHI          42  GACGCC               G[GA]^CG[CT]C
             2012  GGCGTC              
BsaI         3433  GAGACC               GGTCTC(1/5)
             2800  GGTCTC              
BsmI         1894  GAATGC               GAATGC(1/-1)
             2133  GAATGC              
BspDI         431  ATCGAT               AT^CGAT
             2312  ATCGAT              
Bst1107I     5267  GTATAC               GTA^TAC
             5713  GTATAC              
BstEII       2440  GGTAACC              G^GT[ACGT]ACC
             4486  GGTAACC             
BstPI        2440  GGTAACC              G^GT[ACGT]ACC
             4486  GGTAACC             
BstZ17I      5267  GTATAC               GTA^TAC
             5713  GTATAC              
CelII        2663  GCTGAGC              GC^T[ACGT]AGC
             4049  GCTCAGC             
ClaI          431  ATCGAT               AT^CGAT
             2312  ATCGAT              
DraII        3283  GGGTCCT              [GA]G^G[ACGT]CC[CT]
             3571  AGGACCT             
EcoNI        3548  CCTCAAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             6655  CCTTTATCAGG         
EcoO109I     3283  GGGTCCT              [GA]G^G[ACGT]CC[CT]
             3571  AGGACCT             
EcoO65I      2440  GGTAACC              G^GT[ACGT]ACC
             4486  GGTAACC             
Hin1I          42  GACGCC               G[GA]^CG[CT]C
             2012  GGCGTC              
MamI          443  GATGAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2576  GATCTAGATC          
MfeI         1866  CAATTG               C^AATTG
             5135  CAATTG              
MunI         1866  CAATTG               C^AATTG
             5135  CAATTG              
PflFI        2525  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3531  GACGCAGTC           
PpuMI        3283  GGGTCCT              [GA]G^G[AT]CC[CT]
             3571  AGGACCT             
PshBI        5410  ATTAAT               AT^TAAT
             5961  ATTAAT              
SacI         1440  GAGCTC               GAGCT^C
             2339  GAGCTC              
Tth111I      2525  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3531  GACGCAGTC           
XbaI         1183  TCTAGA               T^CTAGA
             2578  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  116 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AluI        AlwI        AlwNI       ApoI        Asp700I     
AspHI       AvaII       BanII       BbsI        BbvI        
BcnI        BfaI        BglII       BmyI        BpmI        
BpuAI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
Bsp1407I    BspMI       BsrDI       BsrGI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        EarI        EcoRII      
EcoRV       EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HincII      HindII      HindIII     HinfI       HpaI        
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PstI        PvuII       RsaI        SapI        Sau3AI      
Sau96I      ScaI        ScrFI       SfaNI       SfcI        
SmlI        SpeI        SphI        SspBI       SspI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
XmnI