##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AccIII AgeI AhdI Alw44I
ApaLI AscI AspEI AviII BbrPI
BciVI BlpI Bpu1102I BsaI BseAI
BsiEI BspDI BspEI BssHII Bst1107I
BstZ17I CelII ClaI CpoI EagI
Eam1105I EclXI Eco52I EcoRI FseI
FspI KspI MluI MroI NaeI
NgoMIV NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PvuI RsrII SacII SalI SbfI
ScaI SfiI SgrAI SnaBI Sse8387I
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 1911 GGTACC G^GTACC
AclI 535 AACGTT AA^CGTT
AcyI 133 GGCGCC G[GA]^CG[CT]C
Aor51HI 2283 AGCGCT AGC^GCT
Asp718I 1911 GGTACC G^GTACC
AspI 1381 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 4929 TGGCCA TGG^CCA
BbeI 133 GGCGCC GGCGC^C
BbvCI 630 CCTCAGC CCTCAGC(-5/-2)
BcgI 2483 GCACAAGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 2428 TACGTG [CT]AC^GT[GA]
BsaBI 4001 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 133 GGCGCC G[GA]^CG[CT]C
BsaWI 1192 TCCGGT [AT]^CCGG[AT]
BsiWI 1059 CGTACG C^GTACG
BsmBI 6134 CGTCTC CGTCTC(1/5)
BspLU11I 4685 ACATGT A^CATGT
BstBI 977 TTCGAA TT^CGAA
BstEII 303 GGTCACC G^GT[ACGT]ACC
BstPI 303 GGTCACC G^GT[ACGT]ACC
DrdI 1378 GACGACAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2283 AGCGCT AGC^GCT
EcoO65I 303 GGTCACC G^GT[ACGT]ACC
EcoRV 1789 GATATC GAT^ATC
Hin1I 133 GGCGCC G[GA]^CG[CT]C
HindIII 3032 AAGCTT A^AGCTT
KasI 133 GGCGCC G^GCGCC
KpnI 1911 GGTACC GGTAC^C
MamI 4001 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3099 CAATTG C^AATTG
MluNI 4929 TGGCCA TGG^CCA
MscI 4929 TGGCCA TGG^CCA
MunI 3099 CAATTG C^AATTG
NarI 133 GGCGCC GG^CGCC
NdeI 4529 CATATG CA^TATG
NspV 977 TTCGAA TT^CGAA
PflFI 1381 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1634 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 535 AACGTT AA^CGTT
SfoI 133 GGCGCC GGC^GCC
SfuI 977 TTCGAA TT^CGAA
SmaI 3134 CCCGGG CCC^GGG
SpeI 1149 ACTAGT A^CTAGT
SspI 970 AATATT AAT^ATT
Tth111I 1381 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1634 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1984 CCAGGCTGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3134 CCCGGG C^CCGGG
XmaI 3134 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 4427 GTAGAC GT^[AC][GT]AC
4592 GTCTAC
AflII 2536 CTTAAG C^TTAAG
3029 CTTAAG
AflIII 2429 ACGTGT A^C[GA][CT]GT
4685 ACATGT
BamHI 1657 GGATCC G^GATCC
2112 GGATCC
BfrI 2536 CTTAAG C^TTAAG
3029 CTTAAG
BglII 1167 AGATCT A^GATCT
5314 AGATCT
BspHI 2140 TCATGA T^CATGA
2999 TCATGA
BsrFI 45 ACCGGC [GA]^CCGG[CT]
1519 ACCGGC
BssSI 1626 CTCGTG CACGAG(-5/-1)
2491 CTCGTG
BstXI 2032 CCAGGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2348 CCAAGTGCATGG
Cfr10I 45 ACCGGC [GA]^CCGG[CT]
1519 ACCGGC
EaeI 4317 CGGCCA [CT]^GGCC[GA]
4929 TGGCCA
HaeII 133 GGCGCC [GA]GCGC^[CT]
2283 AGCGCT
NheI 292 GCTAGC G^CTAGC
430 GCTAGC
RcaI 2140 TCATGA T^CATGA
2999 TCATGA
SexAI 563 ACCAGGT A^CC[AT]GGT
1633 ACCAGGT
SphI 4524 GCATGC GCATG^C
6094 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AseI AsnI Asp700I AspHI AvaI
AvaII AvrII BanI BanII BbsI
BbvI BclI BcnI BfaI BglI
BlnI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrDI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I EcoT22I FauI
FbaI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NsiI NspI PleI PpuMI PshBI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XhoII XmnI