##################################
# NO CUT ENZYMES (   41 enzymes) #
##################################

AatII       AflII       AgeI        Aor51HI     ApaI        
AscI        AviII       AvrII       BalI        BamHI       
BfrI        BlnI        BsaBI       BsiWI       BspLU11I    
Bst1107I    BstXI       BstZ17I     DraIII      DrdI        
Eco47III    FspI        HpaI        MamI        MluI        
MluNI       MscI        NheI        NruI        PacI        
PaeR7I      PinAI       PmeI        PvuI        SbfI        
SexAI       SfiI        SnaBI       Sse8387I    SwaI        
XhoI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

Acc65I       5419  GGTACC               G^GTACC
AclI         2219  AACGTT               AA^CGTT
Alw44I       1935  GTGCAC               G^TGCAC
ApaLI        1935  GTGCAC               G^TGCAC
Asp718I      5419  GGTACC               G^GTACC
AspI         4777  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI        1932  CACGTG               CAC^GTG
BcgI         1274  GCAGGAGTTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        3366  GGATAC               GTATCC(6/5)
BclI          772  TGATCA               T^GATCA
BglII        3551  AGATCT               A^GATCT
BsaAI        1932  CACGTG               [CT]AC^GT[GA]
BsmBI         410  CGTCTC               CGTCTC(1/5)
BsmI          364  GCATTC               GAATGC(1/-1)
BspDI        1011  ATCGAT               AT^CGAT
BssHII        607  GCGCGC               G^CGCGC
BstEII        974  GGTGACC              G^GT[ACGT]ACC
BstPI         974  GGTGACC              G^GT[ACGT]ACC
ClaI         1011  ATCGAT               AT^CGAT
CpoI         1079  CGGTCCG              CG^G[AT]CCG
EcoO65I       974  GGTGACC              G^GT[ACGT]ACC
EcoT22I      4888  ATGCAT               ATGCA^T
FbaI          772  TGATCA               T^GATCA
FseI          312  GGCCGGCC             GGCCGG^CC
HincII       3115  GTCGAC               GT[CT]^[GA]AC
HindII       3115  GTCGAC               GT[CT]^[GA]AC
KpnI         5419  GGTACC               GGTAC^C
NcoI         2779  CCATGG               C^CATGG
NotI            1  GCGGCCGC             GC^GGCCGC
NsiI         4888  ATGCAT               ATGCA^T
PflFI        4777  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1932  CACGTG               CAC^GTG
PmlI         1932  CACGTG               CAC^GTG
PshAI        3310  GACAGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     2219  AACGTT               AA^CGTT
RsrII        1079  CGGTCCG              CG^G[AT]CCG
SalI         3115  GTCGAC               G^TCGAC
ScaI         5656  AGTACT               AGT^ACT
SpeI          660  ACTAGT               A^CTAGT
SspI         3765  AATATT               AAT^ATT
Tth111I      4777  GACATTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         5071  TCTAGA               T^CTAGA
XcmI         1609  CCAGCGAATCTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   38 enzymes) #
###################################

AflIII       1931  ACACGT               A^C[GA][CT]GT
             2269  ACACGT              
AhdI          792  GACCCCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3489  GACATGATGTC         
AocI         1898  CCTGAGG              CC^T[ACGT]AGG
             2678  CCTCAGG             
AseI         4532  ATTAAT               AT^TAAT
             5899  ATTAAT              
AsnI         4532  ATTAAT               AT^TAAT
             5899  ATTAAT              
AspEI         792  GACCCCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3489  GACATGATGTC         
BglI          117  GCCTGCCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              308  GCCCGGCCGGC         
BsiEI           2  CGGCCG               CG[GA][CT]^CG
              311  CGGCCG              
Bsp1407I     2404  TGTACA               T^GTACA
             4460  TGTACA              
BspHI        3536  TCATGA               T^CATGA
             4554  TCATGA              
BsrBI         222  CCGCTC               CCGCTC(-3/-3)
             1239  GAGCGG              
BsrDI        2608  GCAATG               GCAATG(2/0)
             2715  GCAATG              
BsrGI        2404  TGTACA               T^GTACA
             4460  TGTACA              
BstAPI       1721  GCACTCTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             4267  GCAGAAAGTGC         
Bsu36I       1898  CCTGAGG              CC^T[ACGT]AGG
             2678  CCTCAGG             
DraI         1491  TTTAAA               TTT^AAA
             4916  TTTAAA              
EagI            2  CGGCCG               C^GGCCG
              311  CGGCCG              
Eam1105I      792  GACCCCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3489  GACATGATGTC         
EclXI           2  CGGCCG               C^GGCCG
              311  CGGCCG              
Eco52I          2  CGGCCG               C^GGCCG
              311  CGGCCG              
Eco81I       1898  CCTGAGG              CC^T[ACGT]AGG
             2678  CCTCAGG             
EcoRI        2504  GAATTC               G^AATTC
             5123  GAATTC              
EcoRV        1497  GATATC               GAT^ATC
             4767  GATATC              
MfeI         4175  CAATTG               C^AATTG
             5302  CAATTG              
MunI         4175  CAATTG               C^AATTG
             5302  CAATTG              
NaeI          313  GCCGGC               GCC^GGC
              575  GCCGGC              
NdeI         4431  CATATG               CA^TATG
             4601  CATATG              
NgoMIV        313  GCCGGC               G^CCGGC
              575  GCCGGC              
PflMI        1563  CCAGAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4031  CCAGGCCTTGG         
PshBI        4532  ATTAAT               AT^TAAT
             5899  ATTAAT              
RcaI         3536  TCATGA               T^CATGA
             4554  TCATGA              
SacI         2360  GAGCTC               GAGCT^C
             4367  GAGCTC              
SgrAI         574  CGCCGGCG             C[GA]^CCGG[CT]G
             1304  CACCGGCG            
SmaI          429  CCCGGG               CCC^GGG
             1125  CCCGGG              
SspBI        2404  TGTACA               T^GTACA
             4460  TGTACA              
Van91I       1563  CCAGAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4031  CCAGGCCTTGG         
XmaCI         429  CCCGGG               C^CCGGG
             1125  CCCGGG              
XmaI          429  CCCGGG               C^CCGGG
             1125  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  136 enzymes) #
#########################################

AccI        AccII       AccIII      AciI        AcsI        
AcyI        AfaI        AluI        AlwI        AlwNI       
ApoI        Asp700I     AspHI       AvaI        AvaII       
BanI        BanII       BbeI        BbsI        BbvCI       
BbvI        BcnI        BfaI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspEI       BspMI       BsrFI       
BsrI        BssKI       BssSI       BstBI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HindIII     HinfI       
HpaII       HphI        ItaI        KasI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MroI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NarI        NciI        NdeII       NlaIII      
NlaIV       NspI        NspV        PleI        PpuMI       
PstI        PvuII       RsaI        SacII       SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfoI        SfuI        SmlI        SphI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
XmnI