##################################
# NO CUT ENZYMES (   56 enzymes) #
##################################

AclI        AflII       AgeI        AhdI        Alw44I      
ApaLI       AscI        AseI        AsnI        AspEI       
AspI        BbrPI       BclI        BfrI        BspHI       
BspLU11I    Bst1107I    BstBI       BstZ17I     DraI        
Eam1105I    EcoRV       EcoT22I     FbaI        FseI        
KspI        MfeI        MluI        MunI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        SacII       ScaI        SfuI        SgrAI       
SphI        SspI        SwaI        Tth111I     XcmI        
XhoI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AatII        3490  GACGTC               GACGT^C
Acc65I       5327  GGTACC               G^GTACC
AccI          147  GTCGAC               GT^[AC][GT]AC
Asp700I      2046  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      5327  GGTACC               G^GTACC
AviII         781  TGCGCA               TGC^GCA
AvrII        4550  CCTAGG               C^CTAGG
BciVI        3667  GGATAC               GTATCC(6/5)
BglII        2437  AGATCT               A^GATCT
BlnI         4550  CCTAGG               C^CTAGG
BsmI          637  GAATGC               GAATGC(1/-1)
BspDI        5582  ATCGAT               AT^CGAT
BsrDI        5446  CATTGC               GCAATG(2/0)
BssHII        307  GCGCGC               G^CGCGC
BstEII       6301  GGTCACC              G^GT[ACGT]ACC
BstPI        6301  GGTCACC              G^GT[ACGT]ACC
ClaI         5582  ATCGAT               AT^CGAT
CpoI         5294  CGGTCCG              CG^G[AT]CCG
EcoO65I      6301  GGTCACC              G^GT[ACGT]ACC
EcoRI           8  GAATTC               G^AATTC
FspI          781  TGCGCA               TGC^GCA
HindIII      1427  AAGCTT               A^AGCTT
HpaI         2744  GTTAAC               GTT^AAC
KpnI         5327  GGTACC               GGTAC^C
NcoI         5436  CCATGG               C^CATGG
NdeI         6385  CATATG               CA^TATG
RsrII        5294  CGGTCCG              CG^G[AT]CCG
SalI          147  GTCGAC               G^TCGAC
SbfI         4037  CCTGCAGG             CCTGCA^GG
SexAI        2003  ACCTGGT              A^CC[AT]GGT
SfiI         4453  GGCCGACCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI         5372  CTCAAG               C^T[CT][GA]AG
SnaBI        5020  TACGTA               TAC^GTA
SpeI          431  ACTAGT               A^CTAGT
Sse8387I     4037  CCTGCAGG             CCTGCA^GG
XbaI          483  TCTAGA               T^CTAGA
XmnI         2046  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccIII       4851  TCCGGA               T^CCGGA
             5379  TCCGGA              
AflIII       1504  ACACGT               A^C[GA][CT]GT
             5220  ACGTGT              
AocI          423  CCTCAGG              CC^T[ACGT]AGG
             6295  CCTGAGG             
BamHI         758  GGATCC               G^GATCC
             5412  GGATCC              
BcgI         5271  CGAACCGCGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              480  GCATCTAGATCG        
BsaWI        4851  TCCGGA               [AT]^CCGG[AT]
             5379  TCCGGA              
BseAI        4851  TCCGGA               T^CCGGA
             5379  TCCGGA              
BsiWI        1074  CGTACG               C^GTACG
             5022  CGTACG              
Bsp1407I     6097  TGTACA               T^GTACA
             6381  TGTACA              
BspEI        4851  TCCGGA               T^CCGGA
             5379  TCCGGA              
BsrGI        6097  TGTACA               T^GTACA
             6381  TGTACA              
BssSI        5417  CACGAG               CACGAG(-5/-1)
             3273  CTCGTG              
Bsu36I        423  CCTCAGG              CC^T[ACGT]AGG
             6295  CCTGAGG             
DraIII       4935  CACGCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             5590  CACCAAGTG           
EagI          572  CGGCCG               C^GGCCG
             1324  CGGCCG              
EclXI         572  CGGCCG               C^GGCCG
             1324  CGGCCG              
Eco52I        572  CGGCCG               C^GGCCG
             1324  CGGCCG              
Eco81I        423  CCTCAGG              CC^T[ACGT]AGG
             6295  CCTGAGG             
EcoNI        2004  CCTGGTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4399  CCTCACCGAGG         
MroI         4851  TCCGGA               T^CCGGA
             5379  TCCGGA              
NspI         5508  ACATGC               [GA]CATG^[CT]
             5904  ACATGC              
PflMI        1998  CCAGAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4091  CCACCTGCTGG         
SacI         2676  GAGCTC               GAGCT^C
             5860  GAGCTC              
SspBI        6097  TGTACA               T^GTACA
             6381  TGTACA              
Van91I       1998  CCAGAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4091  CCACCTGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  140 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       Aor51HI     ApaI        
ApoI        AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbeI        BbsI        BbvCI       
BbvI        BcnI        BfaI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DrdI        
DsaI        EaeI        EarI        Eco47III    EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        KasI        Ksp632I     
MaeI        MaeII       MaeIII      MamI        MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NarI        NciI        
NdeII       NgoMIV      NlaIII      NlaIV       PleI        
PpuMI       PstI        PvuII       RsaI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfoI        SmaI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       XmaCI       XmaI