##################################
# NO CUT ENZYMES (   52 enzymes) #
##################################

AatII       AclI        AflII       AscI        AseI        
AsnI        AviII       AvrII       BbeI        BbrPI       
BfrI        BlnI        BsiWI       BspDI       BsrDI       
Bst1107I    BstZ17I     ClaI        CpoI        EcoRI       
EcoRV       FseI        FspI        KasI        KspI        
MluI        NarI        NdeI        NheI        NotI        
PacI        PaeR7I      PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        SexAI       SfoI        
SgrAI       SnaBI       SpeI        Sse8387I    SwaI        
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AcsI         3324  GAATTT               [GA]^AATT[CT]
AgeI          499  ACCGGT               A^CCGGT
AhdI         5904  GACACAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2498  GTGCAC               G^TGCAC
Aor51HI      1094  AGCGCT               AGC^GCT
ApaLI        2498  GTGCAC               G^TGCAC
ApoI         3324  GAATTT               [GA]^AATT[CT]
AspEI        5904  GACACAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI        5020  GTATCC               GTATCC(6/5)
BclI          395  TGATCA               T^GATCA
BglII        4622  AGATCT               A^GATCT
BsaAI          18  TACGTG               [CT]AC^GT[GA]
BsaBI        2723  GATGGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI         398  TCATGA               T^CATGA
BssHII        221  GCGCGC               G^CGCGC
BstBI         355  TTCGAA               TT^CGAA
DraI          411  TTTAAA               TTT^AAA
DrdI         5280  GACCGCCAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         5130  CGGCCG               C^GGCCG
Eam1105I     5904  GACACAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        5130  CGGCCG               C^GGCCG
Eco47III     1094  AGCGCT               AGC^GCT
Eco52I       5130  CGGCCG               C^GGCCG
EcoT22I      3585  ATGCAT               ATGCA^T
FbaI          395  TGATCA               T^GATCA
HpaI         1305  GTTAAC               GTT^AAC
MamI         2723  GATGGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         3828  CAATTG               C^AATTG
MunI         3828  CAATTG               C^AATTG
NsiI         3585  ATGCAT               ATGCA^T
NspV          355  TTCGAA               TT^CGAA
PinAI         499  ACCGGT               A^CCGGT
RcaI          398  TCATGA               T^CATGA
ScaI         3104  AGTACT               AGT^ACT
SfuI          355  TTCGAA               TT^CGAA
SmaI         3643  CCCGGG               CCC^GGG
SspI          616  AATATT               AAT^ATT
XmaCI        3643  CCCGGG               C^CCGGG
XmaI         3643  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

Acc65I       3232  GGTACC               G^GTACC
             3467  GGTACC              
AccI         1572  GTAGAC               GT^[AC][GT]AC
             1890  GTCTAC              
AccIII        763  TCCGGA               T^CCGGA
              973  TCCGGA              
Asp700I       140  GAATGGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3728  GAACTGCTTC          
Asp718I      3232  GGTACC               G^GTACC
             3467  GGTACC              
BamHI         766  GGATCC               G^GATCC
             5107  GGATCC              
BcgI          544  CGAGCCTGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             4064  CGAGGAGGCTGC        
BseAI         763  TCCGGA               T^CCGGA
              973  TCCGGA              
BsiEI        4466  CGACCG               CG[GA][CT]^CG
             5130  CGGCCG              
BsmBI        2873  CGTCTC               CGTCTC(1/5)
             2991  GAGACG              
BsmI         7063  GAATGC               GAATGC(1/-1)
             3655  GCATTC              
BspEI         763  TCCGGA               T^CCGGA
              973  TCCGGA              
BstEII       2121  GGTGACC              G^GT[ACGT]ACC
             6690  GGTCACC             
BstPI        2121  GGTGACC              G^GT[ACGT]ACC
             6690  GGTCACC             
EcoO65I      2121  GGTGACC              G^GT[ACGT]ACC
             6690  GGTCACC             
KpnI         3232  GGTACC               GGTAC^C
             3467  GGTACC              
MroI          763  TCCGGA               T^CCGGA
              973  TCCGGA              
NruI           96  TCGCGA               TCG^CGA
              238  TCGCGA              
SapI         6396  GAAGAGC              GCTCTTC(1/4)
             5060  GCTCTTC             
SphI         3710  GCATGC               GCATG^C
             3895  GCATGC              
XmnI          140  GAATGGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3728  GAACTGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  146 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        AlwNI       AocI        ApaI        
AspHI       AspI        AvaI        AvaII       BalI        
BanI        BanII       BbsI        BbvCI       BbvI        
BcnI        BfaI        BglI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaHI       BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    Bsp1407I    BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BsrI        BssKI       BssSI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DraIII      DsaI        EaeI        EarI        Eco81I      
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NcoI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PflFI       PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfiI        
SmlI        SspBI       StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     Van91I      XcmI        
XhoII