##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
Acc65I AflII AgeI AscI AseI
AsnI Asp718I AvrII BciVI BclI
BfrI BlnI BsiEI BsiWI BsmI
BspHI BspLU11I BssSI Bst1107I BstBI
BstEII BstPI BstZ17I EagI EclXI
Eco52I EcoO65I FbaI FseI HpaI
KpnI MluI NdeI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshBI PvuI RcaI SalI
SbfI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 2473 GACGTC GACGT^C
AccI 5508 GTCTAC GT^[AC][GT]AC
AclI 1861 AACGTT AA^CGTT
AhdI 1733 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1733 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3420 TGCGCA TGC^GCA
BamHI 1199 GGATCC G^GATCC
BglII 6106 AGATCT A^GATCT
BsaBI 1296 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 6310 TGTACA T^GTACA
BspDI 232 ATCGAT AT^CGAT
BsrBI 319 CCGCTC CCGCTC(-3/-3)
BsrDI 552 CATTGC GCAATG(2/0)
BsrGI 6310 TGTACA T^GTACA
BssHII 3739 GCGCGC G^CGCGC
BstAPI 5747 GCAGCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 232 ATCGAT AT^CGAT
CpoI 4103 CGGACCG CG^G[AT]CCG
DraI 6287 TTTAAA TTT^AAA
DraIII 3368 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2467 GACATTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1733 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3512 GAATTC G^AATTC
EcoRV 235 GATATC GAT^ATC
EcoT22I 5489 ATGCAT ATGCA^T
FspI 3420 TGCGCA TGC^GCA
MamI 1296 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1182 CAATTG C^AATTG
MunI 1182 CAATTG C^AATTG
NsiI 5489 ATGCAT ATGCA^T
Psp1406I 1861 AACGTT AA^CGTT
RsrII 4103 CGGACCG CG^G[AT]CCG
SacI 2543 GAGCTC GAGCT^C
ScaI 863 AGTACT AGT^ACT
SexAI 495 ACCTGGT A^CC[AT]GGT
SspBI 6310 TGTACA T^GTACA
SspI 1240 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 2371 ACGTGT A^C[GA][CT]GT
5359 ACGTGT
AocI 2799 CCTCAGG CC^T[ACGT]AGG
4427 CCTGAGG
Aor51HI 5025 AGCGCT AGC^GCT
5300 AGCGCT
BsmBI 1394 GAGACG CGTCTC(1/5)
2528 GAGACG
Bsu36I 2799 CCTCAGG CC^T[ACGT]AGG
4427 CCTGAGG
Eco47III 5025 AGCGCT AGC^GCT
5300 AGCGCT
Eco81I 2799 CCTCAGG CC^T[ACGT]AGG
4427 CCTGAGG
HincII 1169 GTCAAC GT[CT]^[GA]AC
5774 GTTGAC
HindII 1169 GTCAAC GT[CT]^[GA]AC
5774 GTTGAC
KspI 83 CCGCGG CCGC^GG
146 CCGCGG
PflMI 2779 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5976 CCAGTCCTTGG
PshAI 2339 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2401 GACTCCGGTC
SacII 83 CCGCGG CCGC^GG
146 CCGCGG
SmaI 938 CCCGGG CCC^GGG
5633 CCCGGG
Van91I 2779 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5976 CCAGTCCTTGG
XbaI 697 TCTAGA T^CTAGA
6130 TCTAGA
XmaCI 938 CCCGGG C^CCGGG
5633 CCCGGG
XmaI 938 CCCGGG C^CCGGG
5633 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 149 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
ApaI ApaLI ApoI Asp700I AspHI
AspI AvaI AvaII BalI BanI
BanII BbeI BbrPI BbsI BbvCI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspEI BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflFI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XcmI XhoII XmnI