##################################
# NO CUT ENZYMES (   71 enzymes) #
##################################

AatII       Acc65I      AflII       AgeI        Alw44I      
Aor51HI     ApaLI       AscI        Asp718I     AviII       
BamHI       BbeI        BbrPI       BbvCI       BciVI       
BfrI        BglI        BsaI        BsiWI       BsmBI       
BsrBI       BssHII      Bst1107I    BstEII      BstPI       
BstZ17I     CpoI        DrdI        EagI        EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRV       
FseI        FspI        HpaI        KasI        KpnI        
KspI        MfeI        MunI        NarI        NheI        
NotI        NruI        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PstI        RsrII       
SacI        SacII       SalI        SbfI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SpeI        
Sse8387I    XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   63 enzymes) #
###################################

AccI         2205  GTAGAC               GT^[AC][GT]AC
AccIII       1595  TCCGGA               T^CCGGA
AclI         4206  AACGTT               AA^CGTT
AhdI         2661  GACTTTATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         3415  CCTCAGG              CC^T[ACGT]AGG
ApaI          275  GGGCCC               GGGCC^C
AspEI        2661  GACTTTATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1985  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI           47  CCCGAG               C^[CT]CG[GA]G
AvrII        2415  CCTAGG               C^CTAGG
BalI         1385  TGGCCA               TGG^CCA
BclI         1614  TGATCA               T^GATCA
BcnI          273  CCGGG                CC^[GC]GG
BglII        2821  AGATCT               A^GATCT
BlnI         2415  CCTAGG               C^CTAGG
BlpI         2715  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I     2715  GCTTAGC              GC^T[ACGT]AGC
BsaAI        1086  TACGTA               [CT]AC^GT[GA]
BsaBI        1753  GATTCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1595  TCCGGA               [AT]^CCGG[AT]
BseAI        1595  TCCGGA               T^CCGGA
BsiEI        3070  CGATCG               CG[GA][CT]^CG
BsoBI          47  CCCGAG               C^[CT]CG[GA]G
BspDI        2947  ATCGAT               AT^CGAT
BspEI        1595  TCCGGA               T^CCGGA
BspHI        3373  TCATGA               T^CATGA
BstBI         509  TTCGAA               TT^CGAA
BstXI         810  CCAGCCAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       3415  CCTCAGG              CC^T[ACGT]AGG
CelII        2715  GCTTAGC              GC^T[ACGT]AGC
ClaI         2947  ATCGAT               AT^CGAT
DraII        2570  GGGACCC              [GA]G^G[ACGT]CC[CT]
DraIII       2015  CACTTAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     2661  GACTTTATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       3415  CCTCAGG              CC^T[ACGT]AGG
EcoO109I     2570  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRI        3582  GAATTC               G^AATTC
FbaI         1614  TGATCA               T^GATCA
MamI         1753  GATTCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI         4188  ACGCGT               A^CGCGT
MluNI        1385  TGGCCA               TGG^CCA
MroI         1595  TCCGGA               T^CCGGA
MscI         1385  TGGCCA               TGG^CCA
NaeI         2711  GCCGGC               GCC^GGC
NciI          273  CCGGG                CC^[GC]GG
NcoI          146  CCATGG               C^CATGG
NdeI         1793  CATATG               CA^TATG
NgoMIV       2711  GCCGGC               G^CCGGC
NspV          509  TTCGAA               TT^CGAA
PflFI        1985  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        2570  GGGACCC              [GA]G^G[AT]CC[CT]
PshAI         823  GACAACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     4206  AACGTT               AA^CGTT
PvuI         3070  CGATCG               CGAT^CG
PvuII         815  CAGCTG               CAG^CTG
RcaI         3373  TCATGA               T^CATGA
ScaI         4329  AGTACT               AGT^ACT
SfuI          509  TTCGAA               TT^CGAA
SnaBI        1086  TACGTA               TAC^GTA
SphI         3904  GCATGC               GCATG^C
StuI          981  AGGCCT               AGG^CCT
SwaI         2951  ATTTAAAT             ATTT^AAAT
Tth111I      1985  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccII           1  CGCG                 CG^CG
             4189  CGCG                
AseI         2355  ATTAAT               AT^TAAT
             3198  ATTAAT              
AsnI         2355  ATTAAT               AT^TAAT
             3198  ATTAAT              
AspHI        3122  GAGCAC               G[AT]GC[AT]^C
             3247  GAGCAC              
BanII          50  GAGCCC               G[GA]GC[CT]^C
              275  GGGCCC              
BbsI         4013  GAAGAC               GAAGAC(2/6)
             3171  GTCTTC              
BcgI         3651  CGACTTTCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              109  GCAGGGGCTTCG        
BpuAI        4013  GAAGAC               GAAGAC(2/6)
             3171  GTCTTC              
BsiHKAI      3122  GAGCAC               G[AT]GC[AT]^C
             3247  GAGCAC              
Bsp1407I     2148  TGTACA               T^GTACA
             4038  TGTACA              
BspLU11I     2039  ACATGT               A^CATGT
             3517  ACATGT              
BsrGI        2148  TGTACA               T^GTACA
             4038  TGTACA              
BssSI         709  CACGAG               CACGAG(-5/-1)
             2343  CTCGTG              
BstUI           1  CGCG                 CG^CG
             4189  CGCG                
CfoI          203  GCGC                 GCG^C
             1835  GCGC                
EaeI         1385  TGGCCA               [CT]^GGCC[GA]
             1823  CGGCCA              
HaeII         202  AGCGCC               [GA]GCGC^[CT]
             1834  AGCGCC              
HhaI          203  GCGC                 GCG^C
             1835  GCGC                
HinP1I        203  GCGC                 G^CGC
             1835  GCGC                
HincII       3046  GTCAAC               GT[CT]^[GA]AC
             3946  GTCAAC              
HindII       3046  GTCAAC               GT[CT]^[GA]AC
             3946  GTCAAC              
HindIII      2000  AAGCTT               A^AGCTT
             3938  AAGCTT              
MvnI            1  CGCG                 CG^CG
             4189  CGCG                
PflMI         966  CCAGTTGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1902  CCATGAACTGG         
PshBI        2355  ATTAAT               AT^TAAT
             3198  ATTAAT              
SspBI        2148  TGTACA               T^GTACA
             4038  TGTACA              
Van91I        966  CCAGTTGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1902  CCATGAACTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   97 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        Asp700I     
AvaII       BanI        BbvI        BfaI        BmyI        
BpmI        BsaHI       BsaJI       BseRI       BsgI        
BsiYI       BslI        BsmAI       BsmFI       BsmI        
Bsp1286I    BspMI       BsrDI       BsrFI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstYI       
Cac8I       Cfr10I      Cfr13I      DdeI        DpnII       
DraI        DsaI        EarI        EcoRII      EcoT14I     
EcoT22I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        Hin1I       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NsiI        
NspI        PleI        RsaI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       XmnI