##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AscI Asp718I AviII BbvCI
BciVI BclI BfrI BglII BsaBI
BsaWI BseAI BsiWI BsmBI BsmI
BspDI BspEI BspHI BsrBI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI EcoNI EcoO65I EcoT22I
FbaI FspI HpaI KpnI KspI
MamI MluI MroI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SexAI SfiI SfuI
SgrAI SnaBI SwaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 5316 CGCG CG^CG
AcyI 1352 GGCGCC G[GA]^CG[CT]C
Alw44I 3402 GTGCAC G^TGCAC
ApaLI 3402 GTGCAC G^TGCAC
AspI 1194 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1352 GGCGCC GGCGC^C
BbrPI 172 CACGTG CAC^GTG
BcgI 5306 GCATCACCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 2028 GCCAGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3110 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3110 GCTCAGC GC^T[ACGT]AGC
BsaAI 172 CACGTG [CT]AC^GT[GA]
BsaHI 1352 GGCGCC G[GA]^CG[CT]C
Bsp1407I 5275 TGTACA T^GTACA
BsrGI 5275 TGTACA T^GTACA
BstUI 5316 CGCG CG^CG
CelII 3110 GCTCAGC GC^T[ACGT]AGC
DrdI 6051 GACAGCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 2616 GATATC GAT^ATC
FseI 2032 GGCCGGCC GGCCGG^CC
HgaI 3966 GCGTC GACGC(5/10)
Hin1I 1352 GGCGCC G[GA]^CG[CT]C
HincII 4197 GTTGAC GT[CT]^[GA]AC
HindII 4197 GTTGAC GT[CT]^[GA]AC
KasI 1352 GGCGCC G^GCGCC
MfeI 6134 CAATTG C^AATTG
MunI 6134 CAATTG C^AATTG
MvnI 5316 CGCG CG^CG
NarI 1352 GGCGCC GG^CGCC
NdeI 4865 CATATG CA^TATG
NheI 5060 GCTAGC G^CTAGC
PflFI 1194 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 172 CACGTG CAC^GTG
PmlI 172 CACGTG CAC^GTG
SbfI 3490 CCTGCAGG CCTGCA^GG
SfoI 1352 GGCGCC GGC^GCC
SmaI 2515 CCCGGG CCC^GGG
SpeI 4937 ACTAGT A^CTAGT
SphI 5536 GCATGC GCATG^C
Sse8387I 3490 CCTGCAGG CCTGCA^GG
SspBI 5275 TGTACA T^GTACA
Tth111I 1194 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2515 CCCGGG C^CCGGG
XmaI 2515 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 1383 GTAGAC GT^[AC][GT]AC
5525 GTAGAC
AhdI 950 GACAGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1386 GACTCGCCGTC
Aor51HI 3777 AGCGCT AGC^GCT
5707 AGCGCT
AseI 2750 ATTAAT AT^TAAT
5111 ATTAAT
AsnI 2750 ATTAAT AT^TAAT
5111 ATTAAT
Asp700I 528 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4714 GAAGTTCTTC
AspEI 950 GACAGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1386 GACTCGCCGTC
AvrII 1092 CCTAGG C^CTAGG
4567 CCTAGG
BalI 3904 TGGCCA TGG^CCA
4349 TGGCCA
BamHI 3162 GGATCC G^GATCC
4692 GGATCC
BlnI 1092 CCTAGG C^CTAGG
4567 CCTAGG
BsiEI 2020 CGGCCG CG[GA][CT]^CG
2035 CGGCCG
EagI 2020 CGGCCG C^GGCCG
2035 CGGCCG
Eam1105I 950 GACAGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1386 GACTCGCCGTC
EclXI 2020 CGGCCG C^GGCCG
2035 CGGCCG
Eco47III 3777 AGCGCT AGC^GCT
5707 AGCGCT
Eco52I 2020 CGGCCG C^GGCCG
2035 CGGCCG
EcoRI 2843 GAATTC G^AATTC
4733 GAATTC
MluNI 3904 TGGCCA TGG^CCA
4349 TGGCCA
MscI 3904 TGGCCA TGG^CCA
4349 TGGCCA
PaeR7I 2051 CTCGAG C^TCGAG
5263 CTCGAG
PflMI 1525 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4253 CCAGGTCTTGG
PshBI 2750 ATTAAT AT^TAAT
5111 ATTAAT
ScaI 1293 AGTACT AGT^ACT
6112 AGTACT
SspI 2723 AATATT AAT^ATT
6157 AATATT
StuI 3155 AGGCCT AGG^CCT
3732 AGGCCT
Van91I 1525 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4253 CCAGGTCTTGG
XbaI 1469 TCTAGA T^CTAGA
4426 TCTAGA
XcmI 238 CCATGAATTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5396 CCATTGGTGAAATGG
XhoI 2051 CTCGAG C^TCGAG
5263 CTCGAG
XmnI 528 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4714 GAAGTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspLU11I BspMI
BsrDI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII