##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

AatII       AclI        AgeI        Aor51HI     ApaI        
AscI        AseI        AsnI        AspI        BbeI        
BbrPI       BcgI        BsiWI       BspDI       BssHII      
Bst1107I    BstBI       BstZ17I     ClaI        CpoI        
EagI        EclXI       Eco47III    Eco52I      EcoRV       
EcoT22I     FseI        HincII      HindII      HpaI        
KasI        MfeI        MluI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RsrII       SalI        
SbfI        SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SwaI        Tth111I     
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccI         4395  GTAGAC               GT^[AC][GT]AC
AccIII       3346  TCCGGA               T^CCGGA
AflII         957  CTTAAG               C^TTAAG
AhdI         5508  GACTCGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI        5508  GACTCGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        4381  TGCGCA               TGC^GCA
AvrII        5813  CCTAGG               C^CTAGG
BbvCI        5384  CCTCAGC              CCTCAGC(-5/-2)
BclI         5723  TGATCA               T^GATCA
BfrI          957  CTTAAG               C^TTAAG
BglI         1123  GCCTGCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        3970  AGATCT               A^GATCT
BlnI         5813  CCTAGG               C^CTAGG
BsaAI        4350  TACGTG               [CT]AC^GT[GA]
BseAI        3346  TCCGGA               T^CCGGA
BsiEI        2283  CGGTCG               CG[GA][CT]^CG
BspEI        3346  TCCGGA               T^CCGGA
BspLU11I     4495  ACATGT               A^CATGT
BsrBI          66  GAGCGG               CCGCTC(-3/-3)
BsrFI        3856  ACCGGC               [GA]^CCGG[CT]
BssSI        3450  CACGAG               CACGAG(-5/-1)
Cfr10I       3856  ACCGGC               [GA]^CCGG[CT]
Eam1105I     5508  GACTCGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        4991  GAATTC               G^AATTC
FbaI         5723  TGATCA               T^GATCA
FspI         4381  TGCGCA               TGC^GCA
HaeII        5330  GGCGCT               [GA]GCGC^[CT]
HindIII      3264  AAGCTT               A^AGCTT
KspI         4127  CCGCGG               CCGC^GG
MroI         3346  TCCGGA               T^CCGGA
PflMI        2669  CCAGGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII        4127  CCGCGG               CCGC^GG
SapI          755  GCTCTTC              GCTCTTC(1/4)
ScaI         2686  AGTACT               AGT^ACT
SphI         5249  GCATGC               GCATG^C
SspI         5911  AATATT               AAT^ATT
Van91I       2669  CCAGGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

Acc65I       3703  GGTACC               G^GTACC
             5769  GGTACC              
AcyI         5060  GACGCC               G[GA]^CG[CT]C
             5270  GGCGTC              
Alw44I        980  GTGCAC               G^TGCAC
             4491  GTGCAC              
ApaLI         980  GTGCAC               G^TGCAC
             4491  GTGCAC              
Asp718I      3703  GGTACC               G^GTACC
             5769  GGTACC              
BamHI        1549  GGATCC               G^GATCC
             4468  GGATCC              
BanI         3703  GGTACC               G^G[CT][GA]CC
             5769  GGTACC              
BsaHI        5060  GACGCC               G[GA]^CG[CT]C
             5270  GGCGTC              
Bsp1407I     3694  TGTACA               T^GTACA
             3709  TGTACA              
BsrGI        3694  TGTACA               T^GTACA
             3709  TGTACA              
BstAPI       3479  GCAGTACATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3637  GCATGAGCTGC         
BstEII       1178  GGTGACC              G^GT[ACGT]ACC
             2880  GGTGACC             
BstPI        1178  GGTGACC              G^GT[ACGT]ACC
             2880  GGTGACC             
DraI         2580  TTTAAA               TTT^AAA
             5907  TTTAAA              
EcoNI        2405  CCTTTTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             5105  CCTGCCTGAGG         
EcoO65I      1178  GGTGACC              G^GT[ACGT]ACC
             2880  GGTGACC             
FauI         5380  CCCGC                CCCGC(4/6)
             4129  GCGGG               
Hin1I        5060  GACGCC               G[GA]^CG[CT]C
             5270  GGCGTC              
KpnI         3703  GGTACC               GGTAC^C
             5769  GGTACC              
NcoI           37  CCATGG               C^CATGG
             2812  CCATGG              
PshAI        1573  GACATCGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             4960  GACAGATGTC          
SspBI        3694  TGTACA               T^GTACA
             3709  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (  132 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       AocI        ApoI        
Asp700I     AspHI       AvaI        AvaII       BalI        
BanII       BbsI        BbvI        BciVI       BcnI        
BfaI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaBI       BsaI        BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    BspHI       BspMI       BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
BstYI       Bsu36I      Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraII       DraIII      
DrdI        DsaI        EaeI        EarI        Eco81I      
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MamI        MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        PpuMI       PstI        
PvuII       RcaI        RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SexAI       SfaNI       SfcI        
SmaI        SmlI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII       XmaCI       
XmaI        XmnI