##################################
# NO CUT ENZYMES (   61 enzymes) #
##################################

AatII       Acc65I      AclI        Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp718I     
BamHI       BbrPI       BbvCI       BsaBI       BsiWI       
BsrBI       BssHII      Bst1107I    BstAPI      BstZ17I     
CpoI        DraIII      EagI        EclXI       Eco47III    
Eco52I      EcoNI       EcoRV       FseI        HgaI        
HpaI        KpnI        KspI        MamI        MfeI        
MluI        MunI        NaeI        NgoMIV      NheI        
NotI        NruI        PflMI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    RsrII       SacII       
SalI        SexAI       SfiI        SgrAI       SmaI        
SnaBI       SwaI        Van91I      XbaI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccIII       2120  TCCGGA               T^CCGGA
AflII        4482  CTTAAG               C^TTAAG
AgeI         3559  ACCGGT               A^CCGGT
AhdI         1305  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI        1242  CAGACTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         1610  GGGCCC               GGGCC^C
AspEI        1305  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1598  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1347  TGCGCA               TGC^GCA
BalI         2502  TGGCCA               TGG^CCA
BcgI         1883  CGAAGTAACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         4482  CTTAAG               C^TTAAG
BglII         625  AGATCT               A^GATCT
BlpI         1200  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1200  GCTGAGC              GC^T[ACGT]AGC
BsaAI        4458  CACGTA               [CT]AC^GT[GA]
BseAI        2120  TCCGGA               T^CCGGA
BsmBI        2560  CGTCTC               CGTCTC(1/5)
Bsp1407I     3638  TGTACA               T^GTACA
BspDI         357  ATCGAT               AT^CGAT
BspEI        2120  TCCGGA               T^CCGGA
BspHI        2267  TCATGA               T^CATGA
BsrFI        3559  ACCGGT               [GA]^CCGG[CT]
BsrGI        3638  TGTACA               T^GTACA
BssSI        1086  CACGAG               CACGAG(-5/-1)
CelII        1200  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       3559  ACCGGT               [GA]^CCGG[CT]
ClaI          357  ATCGAT               AT^CGAT
DrdI         3750  GACATTTTAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     1305  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI         1347  TGCGCA               TGC^GCA
HindIII      4606  AAGCTT               A^AGCTT
MluNI        2502  TGGCCA               TGG^CCA
MroI         2120  TCCGGA               T^CCGGA
MscI         2502  TGGCCA               TGG^CCA
PacI         4592  TTAATTAA             TTAAT^TAA
PaeR7I       3992  CTCGAG               C^TCGAG
PflFI        1598  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        3559  ACCGGT               A^CCGGT
PshAI        3827  GACCGAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI          286  CGATCG               CGAT^CG
RcaI         2267  TCATGA               T^CATGA
SacI         1059  GAGCTC               GAGCT^C
SapI          387  GAAGAGC              GCTCTTC(1/4)
SbfI          380  CCTGCAGG             CCTGCA^GG
ScaI         4341  AGTACT               AGT^ACT
Sse8387I      380  CCTGCAGG             CCTGCA^GG
SspBI        3638  TGTACA               T^GTACA
Tth111I      1598  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1497  CCAACAGTCATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         3992  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AccI         1331  GTAGAC               GT^[AC][GT]AC
             4262  GTAGAC              
AccII         751  CGCG                 CG^CG
             1418  CGCG                
AcyI         1830  GGCGCC               G[GA]^CG[CT]C
             3618  GGCGCC              
AlwI          398  GATCC                GGATC(4/5)
              845  GGATC               
AocI         1672  CCTGAGG              CC^T[ACGT]AGG
             1781  CCTCAGG             
AvaI         2106  CTCGGG               C^[CT]CG[GA]G
             3992  CTCGAG              
AvrII         986  CCTAGG               C^CTAGG
             4141  CCTAGG              
BbeI         1830  GGCGCC               GGCGC^C
             3618  GGCGCC              
BclI         2841  TGATCA               T^GATCA
             4215  TGATCA              
BglI         1394  GCCTGGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2782  GCCCCACTGGC         
BlnI          986  CCTAGG               C^CTAGG
             4141  CCTAGG              
BsaHI        1830  GGCGCC               G[GA]^CG[CT]C
             3618  GGCGCC              
BsiEI           1  CGACCG               CG[GA][CT]^CG
              286  CGATCG              
BsmI         1172  GAATGC               GAATGC(1/-1)
             4558  GCATTC              
BsoBI        2106  CTCGGG               C^[CT]CG[GA]G
             3992  CTCGAG              
BspLU11I       56  ACATGT               A^CATGT
             4564  ACATGT              
BstBI         192  TTCGAA               TT^CGAA
              560  TTCGAA              
BstUI         751  CGCG                 CG^CG
             1418  CGCG                
Bsu36I       1672  CCTGAGG              CC^T[ACGT]AGG
             1781  CCTCAGG             
EaeI         1479  TGGCCG               [CT]^GGCC[GA]
             2502  TGGCCA              
Eco81I       1672  CCTGAGG              CC^T[ACGT]AGG
             1781  CCTCAGG             
EcoRI         351  GAATTC               G^AATTC
             2078  GAATTC              
FbaI         2841  TGATCA               T^GATCA
             4215  TGATCA              
HaeII        1830  GGCGCC               [GA]GCGC^[CT]
             3618  GGCGCC              
Hin1I        1830  GGCGCC               G[GA]^CG[CT]C
             3618  GGCGCC              
KasI         1830  GGCGCC               G^GCGCC
             3618  GGCGCC              
MvnI          751  CGCG                 CG^CG
             1418  CGCG                
NarI         1830  GGCGCC               GG^CGCC
             3618  GGCGCC              
NcoI         1691  CCATGG               C^CATGG
             2588  CCATGG              
NspV          192  TTCGAA               TT^CGAA
              560  TTCGAA              
PstI          381  CTGCAG               CTGCA^G
             4510  CTGCAG              
SfoI         1830  GGCGCC               GGC^GCC
             3618  GGCGCC              
SfuI          192  TTCGAA               TT^CGAA
              560  TTCGAA              
SspI         2037  AATATT               AAT^ATT
             2386  AATATT              


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
ApoI        Asp700I     AspHI       AvaII       BanI        
BanII       BbsI        BbvI        BciVI       BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BsrDI       BsrI        BssKI       BstEII      
BstF5I      BstNI       BstPI       BstXI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraII       DsaI        EarI        EcoO109I    
EcoO65I     EcoRII      EcoT14I     EcoT22I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeI        NdeII       NlaIII      
NlaIV       NsiI        NspI        PleI        PpuMI       
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SpeI        SphI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       XmnI