##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AocI Aor51HI
AscI AseI AsnI AviII BbeI
BbrPI BclI BfrI BsaBI BsaHI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
BstAPI BstUI Bsu36I ClaI CpoI
DraI DraIII DrdI Eco47III Eco81I
EcoRI FbaI FseI FspI Hin1I
HindIII HpaI KasI KspI MamI
MluI MroI MvnI NarI NdeI
NheI NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SapI SbfI SexAI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 1908 GGTACC G^GTACC
AcsI 3099 AAATTT [GA]^AATT[CT]
AhdI 1520 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1346 GTGCAC G^TGCAC
ApaI 1870 GGGCCC GGGCC^C
ApaLI 1346 GTGCAC G^TGCAC
ApoI 3099 AAATTT [GA]^AATT[CT]
Asp718I 1908 GGTACC G^GTACC
AspEI 1520 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1346 GTGCAC G[AT]GC[AT]^C
AspI 3254 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2401 CCTAGG C^CTAGG
BamHI 1012 GGATCC G^GATCC
BcgI 3287 GCAGCACCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 3166 GCCTGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2401 CCTAGG C^CTAGG
BsaAI 766 TACGTG [CT]AC^GT[GA]
BsiEI 2701 CGGCCG CG[GA][CT]^CG
BsiHKAI 1346 GTGCAC G[AT]GC[AT]^C
BsmBI 1017 CGTCTC CGTCTC(1/5)
BsrBI 1822 GAGCGG CCGCTC(-3/-3)
BsrFI 1543 GCCGGC [GA]^CCGG[CT]
BssSI 1717 CACGAG CACGAG(-5/-1)
Bst1107I 1753 GTATAC GTA^TAC
BstEII 2684 GGTGACC G^GT[ACGT]ACC
BstPI 2684 GGTGACC G^GT[ACGT]ACC
BstZ17I 1753 GTATAC GTA^TAC
Cfr10I 1543 GCCGGC [GA]^CCGG[CT]
EagI 2701 CGGCCG C^GGCCG
Eam1105I 1520 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2701 CGGCCG C^GGCCG
Eco52I 2701 CGGCCG C^GGCCG
EcoO65I 2684 GGTGACC G^GT[ACGT]ACC
EcoRV 1634 GATATC GAT^ATC
EcoT22I 2949 ATGCAT ATGCA^T
HgaI 2764 GACGC GACGC(5/10)
KpnI 1908 GGTACC GGTAC^C
MfeI 272 CAATTG C^AATTG
MunI 272 CAATTG C^AATTG
NaeI 1543 GCCGGC GCC^GGC
NcoI 3161 CCATGG C^CATGG
NgoMIV 1543 GCCGGC G^CCGGC
NsiI 2949 ATGCAT ATGCA^T
PflFI 3254 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 491 GACTATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 1837 GTCGAC G^TCGAC
ScaI 1172 AGTACT AGT^ACT
SspI 3368 AATATT AAT^ATT
Tth111I 3254 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 1753 GTATAC GT^[AC][GT]AC
1837 GTCGAC
AfaI 1173 GTAC GT^AC
1909 GTAC
Asp700I 672 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1960 GAATGGCTTC
AvaI 135 CCCGGG C^[CT]CG[GA]G
2857 CCCGGG
BciVI 2996 GGATAC GTATCC(6/5)
3195 GGATAC
BglII 859 AGATCT A^GATCT
876 AGATCT
BsoBI 135 CCCGGG C^[CT]CG[GA]G
2857 CCCGGG
BsrDI 1665 CATTGC GCAATG(2/0)
330 GCAATG
BstBI 1060 TTCGAA TT^CGAA
3295 TTCGAA
BstXI 427 CCAGAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2733 CCACAACCTTGG
EcoNI 1085 CCTGCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1945 CCTGACTCAGG
FauI 2491 CCCGC CCCGC(4/6)
2545 CCCGC
HincII 341 GTCAAC GT[CT]^[GA]AC
1837 GTCGAC
HindII 341 GTCAAC GT[CT]^[GA]AC
1837 GTCGAC
MseI 15 TTAA T^TAA
702 TTAA
NspV 1060 TTCGAA TT^CGAA
3295 TTCGAA
PvuII 708 CAGCTG CAG^CTG
1443 CAGCTG
RsaI 1173 GTAC GT^AC
1909 GTAC
SfuI 1060 TTCGAA TT^CGAA
3295 TTCGAA
SmaI 135 CCCGGG CCC^GGG
2857 CCCGGG
SmlI 684 CTCAAG C^T[CT][GA]AG
2140 CTTGAG
Tru9I 15 TTAA T^TAA
702 TTAA
XmaCI 135 CCCGGG C^CCGGG
2857 CCCGGG
XmaI 135 CCCGGG C^CCGGG
2857 CCCGGG
XmnI 672 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1960 GAATGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII