##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AccIII AclI AcsI AflII AgeI
AocI Aor51HI ApoI AscI AseI
AsnI AspI AvrII BbeI BbrPI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsaWI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BsrGI BstBI Bsu36I ClaI DraI
Eco47III Eco81I EcoRI EcoRV EcoT22I
FbaI FseI HindIII HpaI KasI
MamI MfeI MluI MroI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
SapI ScaI SexAI SfoI SfuI
SnaBI SpeI SphI SspBI SspI
SwaI Tru9I Tth111I XbaI XcmI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 1943 GACGTC GACGT^C
Acc65I 1508 GGTACC G^GTACC
AflIII 3058 ACATGT A^C[GA][CT]GT
AhdI 1706 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2940 GTGCAC G^TGCAC
ApaLI 2940 GTGCAC G^TGCAC
Asp700I 937 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1508 GGTACC G^GTACC
AspEI 1706 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1155 TGCGCA TGC^GCA
BamHI 1660 GGATCC G^GATCC
BbsI 1594 GTCTTC GAAGAC(2/6)
BpuAI 1594 GTCTTC GAAGAC(2/6)
BspLU11I 3058 ACATGT A^CATGT
BspMI 1673 GCAGGT ACCTGC(4/8)
BsrDI 1459 CATTGC GCAATG(2/0)
BssHII 22 GCGCGC G^CGCGC
BssSI 994 CACGAG CACGAG(-5/-1)
Bst1107I 1819 GTATAC GTA^TAC
BstXI 2196 CCAAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1819 GTATAC GTA^TAC
CpoI 566 CGGACCG CG^G[AT]CCG
DrdI 258 GACGTGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2106 CGGCCG C^GGCCG
Eam1105I 1706 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 116 GAAGAG CTCTTC(1/4)
EclXI 2106 CGGCCG C^GGCCG
Eco52I 2106 CGGCCG C^GGCCG
FspI 1155 TGCGCA TGC^GCA
HaeII 2557 GGCGCT [GA]GCGC^[CT]
HincII 1538 GTCGAC GT[CT]^[GA]AC
HindII 1538 GTCGAC GT[CT]^[GA]AC
KpnI 1508 GGTACC GGTAC^C
Ksp632I 116 GAAGAG CTCTTC(1/4)
KspI 1270 CCGCGG CCGC^GG
NruI 379 TCGCGA TCG^CGA
NspI 3058 ACATGT [GA]CATG^[CT]
PaeR7I 2330 CTCGAG C^TCGAG
PpuMI 1675 AGGTCCC [GA]G^G[AT]CC[CT]
RsrII 566 CGGACCG CG^G[AT]CCG
SacI 1853 GAGCTC GAGCT^C
SacII 1270 CCGCGG CCGC^GG
SalI 1538 GTCGAC G^TCGAC
SfiI 1468 GGCCCAGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2612 CACCGGCG C[GA]^CCGG[CT]G
XhoI 2330 CTCGAG C^TCGAG
XmnI 937 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BbvCI 202 GCTGAGG CCTCAGC(-5/-2)
640 GCTGAGG
BcgI 932 CGAGAGAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2788 GCAGCCTACTCG
BlpI 38 GCTGAGC GC^T[ACGT]AGC
1036 GCTGAGC
Bpu1102I 38 GCTGAGC GC^T[ACGT]AGC
1036 GCTGAGC
BsaI 339 GAGACC GGTCTC(1/5)
1967 GAGACC
BsmBI 1945 CGTCTC CGTCTC(1/5)
537 GAGACG
BsrBI 304 CCGCTC CCGCTC(-3/-3)
2483 GAGCGG
BstEII 2962 GGTCACC G^GT[ACGT]ACC
3007 GGTGACC
BstPI 2962 GGTCACC G^GT[ACGT]ACC
3007 GGTGACC
CelII 38 GCTGAGC GC^T[ACGT]AGC
1036 GCTGAGC
DraIII 698 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1020 CACTGGGTG
EcoO65I 2962 GGTCACC G^GT[ACGT]ACC
3007 GGTGACC
SbfI 124 CCTGCAGG CCTGCA^GG
1327 CCTGCAGG
SmaI 106 CCCGGG CCC^GGG
463 CCCGGG
Sse8387I 124 CCTGCAGG CCTGCA^GG
1327 CCTGCAGG
XmaCI 106 CCCGGG C^CCGGG
463 CCCGGG
XmaI 106 CCCGGG C^CCGGG
463 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BglI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII