##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI AhdI Aor51HI ApoI
AscI AseI AsnI AspEI AspI
AviII AvrII BbeI BbvCI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BseAI BsiWI BsmBI BspEI
BsrBI BsrDI Bst1107I BstAPI BstZ17I
CelII CpoI Eam1105I Eco47III EcoRI
EcoRV EcoT22I FseI FspI HincII
HindII HpaI KasI MamI MfeI
MluI MroI MunI NarI NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI SbfI
SfiI SfoI SgrAI SnaBI Sse8387I
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 1151 GGTACC G^GTACC
AcyI 2559 GGCGTC G[GA]^CG[CT]C
Asp718I 1151 GGTACC G^GTACC
BamHI 1040 GGATCC G^GATCC
BbrPI 502 CACGTG CAC^GTG
BbsI 820 GAAGAC GAAGAC(2/6)
BpuAI 820 GAAGAC GAAGAC(2/6)
BsaHI 2559 GGCGTC G[GA]^CG[CT]C
BsaI 2662 GAGACC GGTCTC(1/5)
BsaWI 2297 ACCGGA [AT]^CCGG[AT]
BsiEI 11 CGGCCG CG[GA][CT]^CG
Bsp1407I 1098 TGTACA T^GTACA
BspDI 949 ATCGAT AT^CGAT
BspHI 1496 TCATGA T^CATGA
BsrFI 1225 GCCGGC [GA]^CCGG[CT]
BsrGI 1098 TGTACA T^GTACA
BssHII 1002 GCGCGC G^CGCGC
BstBI 2034 TTCGAA TT^CGAA
BstEII 2496 GGTCACC G^GT[ACGT]ACC
BstPI 2496 GGTCACC G^GT[ACGT]ACC
BstXI 1030 CCAGACTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1225 GCCGGC [GA]^CCGG[CT]
ClaI 949 ATCGAT AT^CGAT
DraIII 2675 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 11 CGGCCG C^GGCCG
EclXI 11 CGGCCG C^GGCCG
Eco52I 11 CGGCCG C^GGCCG
EcoNI 678 CCTCTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2496 GGTCACC G^GT[ACGT]ACC
HaeII 2762 AGCGCC [GA]GCGC^[CT]
Hin1I 2559 GGCGTC G[GA]^CG[CT]C
HindIII 1214 AAGCTT A^AGCTT
KpnI 1151 GGTACC GGTAC^C
NaeI 1225 GCCGGC GCC^GGC
NdeI 658 CATATG CA^TATG
NgoMIV 1225 GCCGGC G^CCGGC
NspV 2034 TTCGAA TT^CGAA
PmaCI 502 CACGTG CAC^GTG
PmlI 502 CACGTG CAC^GTG
PpuMI 2483 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 1496 TCATGA T^CATGA
ScaI 572 AGTACT AGT^ACT
SexAI 2676 ACCAGGT A^CC[AT]GGT
SfuI 2034 TTCGAA TT^CGAA
SmaI 238 CCCGGG CCC^GGG
SpeI 3546 ACTAGT A^CTAGT
SphI 2141 GCATGC GCATG^C
SspBI 1098 TGTACA T^GTACA
SspI 3474 AATATT AAT^ATT
TfiI 2551 GAATC G^A[AT]TC
XmaCI 238 CCCGGG C^CCGGG
XmaI 238 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Alw44I 1608 GTGCAC G^TGCAC
2960 GTGCAC
AlwNI 868 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2281 CAGGCACTG
ApaLI 1608 GTGCAC G^TGCAC
2960 GTGCAC
Asp700I 418 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3037 GAAGGGGTTC
BclI 353 TGATCA T^GATCA
1182 TGATCA
BglI 2275 GCCCTTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2833 GCCACCGAGGC
BsmI 1221 GAATGC GAATGC(1/-1)
983 GCATTC
BspMI 2129 ACCTGC ACCTGC(4/8)
3515 ACCTGC
BstYI 1040 GGATCC [GA]^GATC[CT]
2201 AGATCC
DraI 2257 TTTAAA TTT^AAA
3297 TTTAAA
DrdI 2730 GACGCAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3199 GACACAGTTGTC
FbaI 353 TGATCA T^GATCA
1182 TGATCA
KspI 224 CCGCGG CCGC^GG
2804 CCGCGG
MflI 1040 GGATCC [GA]^GATC[CT]
2201 AGATCC
MslI 502 CACGTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2610 CATCAAAGTG
PflMI 1523 CCAACATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3120 CCAGGATGTGG
SacII 224 CCGCGG CCGC^GG
2804 CCGCGG
SmlI 607 CTTGAG C^T[CT][GA]AG
730 CTTGAG
Van91I 1523 CCAACATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3120 CCAGGATGTGG
XhoII 1040 GGATCC [GA]^GATC[CT]
2201 AGATCC
XmnI 418 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3037 GAAGGGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbvI
BcgI BcnI BfaI BmyI BpmI
BsaAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BsrI BssKI BssSI
BstF5I BstNI BstUI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI