################################## # NO CUT ENZYMES ( 48 enzymes) # ################################## AatII AccIII AflII AgeI AhdI ApaI AscI AspEI BamHI BciVI BfrI BseAI BsiEI BsmBI BspEI BspHI BstBI CpoI DrdI EagI Eam1105I EclXI Eco52I EcoRV FseI HpaI MroI NdeI NheI NotI NruI NspV PacI PinAI PmeI PshAI PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SfiI SfuI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### Acc65I 419 GGTACC G^GTACC AclI 5244 AACGTT AA^CGTT Aor51HI 1092 AGCGCT AGC^GCT Asp718I 419 GGTACC G^GTACC AviII 2034 TGCGCA TGC^GCA BlpI 548 GCTGAGC GC^T[ACGT]AGC Bpu1102I 548 GCTGAGC GC^T[ACGT]AGC BsaBI 2167 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2851 ACCGGA [AT]^CCGG[AT] BsiWI 301 CGTACG C^GTACG BspDI 2631 ATCGAT AT^CGAT BstEII 331 GGTGACC G^GT[ACGT]ACC BstPI 331 GGTGACC G^GT[ACGT]ACC CelII 548 GCTGAGC GC^T[ACGT]AGC ClaI 2631 ATCGAT AT^CGAT Eco47III 1092 AGCGCT AGC^GCT EcoO65I 331 GGTGACC G^GT[ACGT]ACC EcoRI 4792 GAATTC G^AATTC EcoT22I 942 ATGCAT ATGCA^T FspI 2034 TGCGCA TGC^GCA KpnI 419 GGTACC GGTAC^C MamI 2167 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 2089 ACGCGT A^CGCGT NcoI 1470 CCATGG C^CATGG NsiI 942 ATGCAT ATGCA^T PaeR7I 4465 CTCGAG C^TCGAG Psp1406I 5244 AACGTT AA^CGTT SgrAI 99 CGCCGGCG C[GA]^CCGG[CT]G SnaBI 4519 TACGTA TAC^GTA StuI 2004 AGGCCT AGG^CCT XcmI 1573 CCAGCAGTCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 4465 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### Alw44I 713 GTGCAC G^TGCAC 1106 GTGCAC AocI 4118 CCTAAGG CC^T[ACGT]AGG 4752 CCTAAGG ApaLI 713 GTGCAC G^TGCAC 1106 GTGCAC Asp700I 1540 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4025 GAATGATTTC AspI 3221 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4676 GACGTAGTC AvrII 1414 CCTAGG C^CTAGG 2176 CCTAGG BbrPI 740 CACGTG CAC^GTG 4735 CACGTG BglI 346 GCCGCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 649 GCCAGGGTGGC BlnI 1414 CCTAGG C^CTAGG 2176 CCTAGG BsaI 772 GAGACC GGTCTC(1/5) 1335 GAGACC Bsp1407I 5315 TGTACA T^GTACA 5366 TGTACA BspLU11I 4301 ACATGT A^CATGT 5085 ACATGT BspMI 1758 ACCTGC ACCTGC(4/8) 4050 ACCTGC BsrBI 3839 CCGCTC CCGCTC(-3/-3) 415 GAGCGG BsrDI 4428 CATTGC GCAATG(2/0) 2063 GCAATG BsrGI 5315 TGTACA T^GTACA 5366 TGTACA Bst1107I 3153 GTATAC GTA^TAC 4273 GTATAC BstXI 4191 CCAGTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4761 CCAGCTCGCTGG BstZ17I 3153 GTATAC GTA^TAC 4273 GTATAC Bsu36I 4118 CCTAAGG CC^T[ACGT]AGG 4752 CCTAAGG Eco81I 4118 CCTAAGG CC^T[ACGT]AGG 4752 CCTAAGG KspI 15 CCGCGG CCGC^GG 222 CCGCGG MfeI 1346 CAATTG C^AATTG 4324 CAATTG MunI 1346 CAATTG C^AATTG 4324 CAATTG PflFI 3221 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4676 GACGTAGTC PmaCI 740 CACGTG CAC^GTG 4735 CACGTG PmlI 740 CACGTG CAC^GTG 4735 CACGTG SacII 15 CCGCGG CCGC^GG 222 CCGCGG SexAI 3594 ACCTGGT A^CC[AT]GGT 4397 ACCTGGT SmaI 195 CCCGGG CCC^GGG 236 CCCGGG SpeI 1393 ACTAGT A^CTAGT 2695 ACTAGT SphI 975 GCATGC GCATG^C 1015 GCATGC SspBI 5315 TGTACA T^GTACA 5366 TGTACA SspI 707 AATATT AAT^ATT 2965 AATATT Tth111I 3221 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4676 GACGTAGTC XbaI 3375 TCTAGA T^CTAGA 3495 TCTAGA XmaCI 195 CCCGGG C^CCGGG 236 CCCGGG XmaI 195 CCCGGG C^CCGGG 236 CCCGGG XmnI 1540 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4025 GAATGATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 139 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI AseI AsnI AspHI AvaI AvaII BalI BanI BanII BbeI BbsI BbvCI BbvI BcgI BclI BcnI BfaI BglII BmyI BpmI BpuAI BsaAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrFI BsrI BssHII BssKI BssSI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PshBI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII