##################################
# NO CUT ENZYMES (   48 enzymes) #
##################################

AatII       AccIII      AflII       AgeI        AhdI        
ApaI        AscI        AspEI       BamHI       BciVI       
BfrI        BseAI       BsiEI       BsmBI       BspEI       
BspHI       BstBI       CpoI        DrdI        EagI        
Eam1105I    EclXI       Eco52I      EcoRV       FseI        
HpaI        MroI        NdeI        NheI        NotI        
NruI        NspV        PacI        PinAI       PmeI        
PshAI       PvuI        RcaI        RsrII       SacI        
SalI        SapI        SbfI        ScaI        SfiI        
SfuI        Sse8387I    SwaI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

Acc65I        419  GGTACC               G^GTACC
AclI         5244  AACGTT               AA^CGTT
Aor51HI      1092  AGCGCT               AGC^GCT
Asp718I       419  GGTACC               G^GTACC
AviII        2034  TGCGCA               TGC^GCA
BlpI          548  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      548  GCTGAGC              GC^T[ACGT]AGC
BsaBI        2167  GATGAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        2851  ACCGGA               [AT]^CCGG[AT]
BsiWI         301  CGTACG               C^GTACG
BspDI        2631  ATCGAT               AT^CGAT
BstEII        331  GGTGACC              G^GT[ACGT]ACC
BstPI         331  GGTGACC              G^GT[ACGT]ACC
CelII         548  GCTGAGC              GC^T[ACGT]AGC
ClaI         2631  ATCGAT               AT^CGAT
Eco47III     1092  AGCGCT               AGC^GCT
EcoO65I       331  GGTGACC              G^GT[ACGT]ACC
EcoRI        4792  GAATTC               G^AATTC
EcoT22I       942  ATGCAT               ATGCA^T
FspI         2034  TGCGCA               TGC^GCA
KpnI          419  GGTACC               GGTAC^C
MamI         2167  GATGAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI         2089  ACGCGT               A^CGCGT
NcoI         1470  CCATGG               C^CATGG
NsiI          942  ATGCAT               ATGCA^T
PaeR7I       4465  CTCGAG               C^TCGAG
Psp1406I     5244  AACGTT               AA^CGTT
SgrAI          99  CGCCGGCG             C[GA]^CCGG[CT]G
SnaBI        4519  TACGTA               TAC^GTA
StuI         2004  AGGCCT               AGG^CCT
XcmI         1573  CCAGCAGTCAAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         4465  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   39 enzymes) #
###################################

Alw44I        713  GTGCAC               G^TGCAC
             1106  GTGCAC              
AocI         4118  CCTAAGG              CC^T[ACGT]AGG
             4752  CCTAAGG             
ApaLI         713  GTGCAC               G^TGCAC
             1106  GTGCAC              
Asp700I      1540  GAATTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4025  GAATGATTTC          
AspI         3221  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4676  GACGTAGTC           
AvrII        1414  CCTAGG               C^CTAGG
             2176  CCTAGG              
BbrPI         740  CACGTG               CAC^GTG
             4735  CACGTG              
BglI          346  GCCGCTGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              649  GCCAGGGTGGC         
BlnI         1414  CCTAGG               C^CTAGG
             2176  CCTAGG              
BsaI          772  GAGACC               GGTCTC(1/5)
             1335  GAGACC              
Bsp1407I     5315  TGTACA               T^GTACA
             5366  TGTACA              
BspLU11I     4301  ACATGT               A^CATGT
             5085  ACATGT              
BspMI        1758  ACCTGC               ACCTGC(4/8)
             4050  ACCTGC              
BsrBI        3839  CCGCTC               CCGCTC(-3/-3)
              415  GAGCGG              
BsrDI        4428  CATTGC               GCAATG(2/0)
             2063  GCAATG              
BsrGI        5315  TGTACA               T^GTACA
             5366  TGTACA              
Bst1107I     3153  GTATAC               GTA^TAC
             4273  GTATAC              
BstXI        4191  CCAGTCAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4761  CCAGCTCGCTGG        
BstZ17I      3153  GTATAC               GTA^TAC
             4273  GTATAC              
Bsu36I       4118  CCTAAGG              CC^T[ACGT]AGG
             4752  CCTAAGG             
Eco81I       4118  CCTAAGG              CC^T[ACGT]AGG
             4752  CCTAAGG             
KspI           15  CCGCGG               CCGC^GG
              222  CCGCGG              
MfeI         1346  CAATTG               C^AATTG
             4324  CAATTG              
MunI         1346  CAATTG               C^AATTG
             4324  CAATTG              
PflFI        3221  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4676  GACGTAGTC           
PmaCI         740  CACGTG               CAC^GTG
             4735  CACGTG              
PmlI          740  CACGTG               CAC^GTG
             4735  CACGTG              
SacII          15  CCGCGG               CCGC^GG
              222  CCGCGG              
SexAI        3594  ACCTGGT              A^CC[AT]GGT
             4397  ACCTGGT             
SmaI          195  CCCGGG               CCC^GGG
              236  CCCGGG              
SpeI         1393  ACTAGT               A^CTAGT
             2695  ACTAGT              
SphI          975  GCATGC               GCATG^C
             1015  GCATGC              
SspBI        5315  TGTACA               T^GTACA
             5366  TGTACA              
SspI          707  AATATT               AAT^ATT
             2965  AATATT              
Tth111I      3221  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4676  GACGTAGTC           
XbaI         3375  TCTAGA               T^CTAGA
             3495  TCTAGA              
XmaCI         195  CCCGGG               C^CCGGG
              236  CCCGGG              
XmaI          195  CCCGGG               C^CCGGG
              236  CCCGGG              
XmnI         1540  GAATTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4025  GAATGATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  139 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AflIII      AluI        AlwI        AlwNI       
ApoI        AseI        AsnI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbeI        
BbsI        BbvCI       BbvI        BcgI        BclI        
BcnI        BfaI        BglII       BmyI        BpmI        
BpuAI       BsaAI       BsaHI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BsrFI       
BsrI        BssHII      BssKI       BssSI       BstAPI      
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraI        DraII       DraIII      DsaI        EaeI        
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        FbaI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HinfI       
HpaII       HphI        ItaI        KasI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NdeII       
NgoMIV      NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PshBI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XhoII