##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI AhdI Alw44I ApaLI AscI
Asp700I Asp718I AspEI AspI AviII
AvrII BbeI BbrPI BbvCI BciVI
BcnI BglI BlnI BsaAI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BsrBI BsrFI BsrGI
BssHII BstBI BstEII BstPI BstUI
Cfr10I ClaI DraIII EagI Eam1105I
EclXI Eco52I EcoO65I EcoRV FauI
FseI FspI HapII HincII HindII
HpaI HpaII KasI KpnI KspI
MluI MroI MspI MvnI NaeI
NarI NciI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccI 706 GTATAC GT^[AC][GT]AC
AcyI 2667 GACGCC G[GA]^CG[CT]C
AflII 1619 CTTAAG C^TTAAG
Aor51HI 3656 AGCGCT AGC^GCT
AvaI 1419 CTCGGG C^[CT]CG[GA]G
BamHI 2840 GGATCC G^GATCC
BfrI 1619 CTTAAG C^TTAAG
BsaBI 5419 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2667 GACGCC G[GA]^CG[CT]C
BsmI 1127 GCATTC GAATGC(1/-1)
BsoBI 1419 CTCGGG C^[CT]CG[GA]G
BspLU11I 5161 ACATGT A^CATGT
BssSI 440 CTCGTG CACGAG(-5/-1)
Bst1107I 706 GTATAC GTA^TAC
BstZ17I 706 GTATAC GTA^TAC
CpoI 1837 CGGACCG CG^G[AT]CCG
DsaI 1347 CCATGG C^C[GA][CT]GG
Eco47III 3656 AGCGCT AGC^GCT
EcoRI 5041 GAATTC G^AATTC
HaeII 3656 AGCGCT [GA]GCGC^[CT]
Hin1I 2667 GACGCC G[GA]^CG[CT]C
MamI 5419 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1347 CCATGG C^CATGG
NheI 5218 GCTAGC G^CTAGC
PshAI 2331 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 1837 CGGACCG CG^G[AT]CCG
SacI 2030 GAGCTC GAGCT^C
SexAI 744 ACCAGGT A^CC[AT]GGT
SpeI 784 ACTAGT A^CTAGT
StuI 1356 AGGCCT AGG^CCT
TaqI 1453 TCGA T^CGA
TthHB8I 1453 TCGA T^CGA
XcmI 1668 CCAAATATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 3005 ACACGT A^C[GA][CT]GT
5161 ACATGT
AlwNI 1255 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1858 CAGGAGCTG
ApaI 300 GGGCCC GGGCC^C
3063 GGGCCC
AspHI 437 GTGCTC G[AT]GC[AT]^C
2030 GAGCTC
BanI 4112 GGCACC G^G[CT][GA]CC
5505 GGTGCC
BcgI 1812 CGAATGAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
286 GCACACAATTCG
BglII 994 AGATCT A^GATCT
2024 AGATCT
BlpI 2045 GCTGAGC GC^T[ACGT]AGC
3125 GCTCAGC
Bpu1102I 2045 GCTGAGC GC^T[ACGT]AGC
3125 GCTCAGC
BsiHKAI 437 GTGCTC G[AT]GC[AT]^C
2030 GAGCTC
BspMI 2200 ACCTGC ACCTGC(4/8)
2967 ACCTGC
BsrDI 1231 CATTGC GCAATG(2/0)
3133 CATTGC
BstAPI 4172 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4475 GCACTTTATGC
CelII 2045 GCTGAGC GC^T[ACGT]AGC
3125 GCTCAGC
DrdI 2422 GACCACACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2966 GACCTGCCTGTC
HgaI 2667 GACGC GACGC(5/10)
5222 GCGTC
MaeII 2598 ACGT A^CGT
3007 ACGT
MfeI 206 CAATTG C^AATTG
4993 CAATTG
MspA1I 266 CAGCTG C[AC]G^C[GT]G
1196 CAGCTG
MunI 206 CAATTG C^AATTG
4993 CAATTG
NdeI 571 CATATG CA^TATG
5563 CATATG
PvuII 266 CAGCTG CAG^CTG
1196 CAGCTG
SapI 2261 GAAGAGC GCTCTTC(1/4)
2270 GAAGAGC
SwaI 3241 ATTTAAAT ATTT^AAAT
4839 ATTTAAAT
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI AseI AsnI AvaII
BalI BanII BbsI BbvI BclI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
EcoT22I FbaI Fnu4HI FokI HaeIII
HhaI HinP1I HindIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV NsiI NspI PleI
PpuMI PshBI PstI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII