##################################
# NO CUT ENZYMES (   66 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AflII       
AhdI        Alw44I      ApaLI       AscI        Asp718I     
AspEI       AspI        AviII       AvrII       BbrPI       
BcgI        BclI        BfrI        BlnI        BsaAI       
BsaBI       BsaI        BseAI       BspDI       BspEI       
BsrDI       Bst1107I    BstAPI      BstBI       BstZ17I     
ClaI        CpoI        DrdI        Eam1105I    EcoRI       
EcoRV       FbaI        FseI        FspI        HpaI        
KpnI        MamI        MluI        MroI        NheI        
NotI        NspV        PacI        PflFI       PmaCI       
PmeI        PmlI        PshAI       PvuI        RsrII       
SalI        SapI        SbfI        SfuI        SgrAI       
SnaBI       SphI        Sse8387I    SwaI        Tth111I     
XbaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AclI         3467  AACGTT               AA^CGTT
AgeI          846  ACCGGT               A^CCGGT
AocI         4778  CCTTAGG              CC^T[ACGT]AGG
AseI         2655  ATTAAT               AT^TAAT
AsnI         2655  ATTAAT               AT^TAAT
BbvCI        2944  GCTGAGG              CCTCAGC(-5/-2)
BglII        2564  AGATCT               A^GATCT
BlpI          764  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      764  GCTGAGC              GC^T[ACGT]AGC
BsaWI         846  ACCGGT               [AT]^CCGG[AT]
BsiWI        4010  CGTACG               C^GTACG
BsmBI        2355  CGTCTC               CGTCTC(1/5)
BsmI         3024  GCATTC               GAATGC(1/-1)
BspHI        2833  TCATGA               T^CATGA
BssSI         515  CACGAG               CACGAG(-5/-1)
Bsu36I       4778  CCTTAGG              CC^T[ACGT]AGG
CelII         764  GCTGAGC              GC^T[ACGT]AGC
DraIII       1661  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I       4778  CCTTAGG              CC^T[ACGT]AGG
HincII       1768  GTTGAC               GT[CT]^[GA]AC
HindII       1768  GTTGAC               GT[CT]^[GA]AC
HindIII      4202  AAGCTT               A^AGCTT
MfeI         1994  CAATTG               C^AATTG
MunI         1994  CAATTG               C^AATTG
NcoI         1393  CCATGG               C^CATGG
NruI         5006  TCGCGA               TCG^CGA
PaeR7I       1443  CTCGAG               C^TCGAG
PinAI         846  ACCGGT               A^CCGGT
PpuMI         914  AGGACCC              [GA]G^G[AT]CC[CT]
PshBI        2655  ATTAAT               AT^TAAT
Psp1406I     3467  AACGTT               AA^CGTT
PstI         2192  CTGCAG               CTGCA^G
RcaI         2833  TCATGA               T^CATGA
ScaI         4048  AGTACT               AGT^ACT
SexAI        3932  ACCAGGT              A^CC[AT]GGT
SfiI          484  GGCCGAGCCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI         2475  ACTAGT               A^CTAGT
StuI         2708  AGGCCT               AGG^CCT
XcmI         1058  CCAGGCATCCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1443  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AlwNI         159  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1244  CAGCGACTG           
Aor51HI        23  AGCGCT               AGC^GCT
             1486  AGCGCT              
BalI         1326  TGGCCA               TGG^CCA
             1802  TGGCCA              
BciVI        2609  GGATAC               GTATCC(6/5)
             4311  GGATAC              
BsgI         4069  CTGCAC               GTGCAG(16/14)
             4908  CTGCAC              
BsrBI          89  GAGCGG               CCGCTC(-3/-3)
              635  GAGCGG              
BssHII        314  GCGCGC               G^CGCGC
              646  GCGCGC              
BstEII       1676  GGTGACC              G^GT[ACGT]ACC
             3525  GGTCACC             
BstPI        1676  GGTGACC              G^GT[ACGT]ACC
             3525  GGTCACC             
BstXI        2076  CCAGGGGCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2722  CCAGAGCTCTGG        
Eco47III       23  AGCGCT               AGC^GCT
             1486  AGCGCT              
EcoO65I      1676  GGTGACC              G^GT[ACGT]ACC
             3525  GGTCACC             
EcoT22I      2390  ATGCAT               ATGCA^T
             2551  ATGCAT              
MluNI        1326  TGGCCA               TGG^CCA
             1802  TGGCCA              
MscI         1326  TGGCCA               TGG^CCA
             1802  TGGCCA              
NsiI         2390  ATGCAT               ATGCA^T
             2551  ATGCAT              
SmlI         1443  CTCGAG               C^T[CT][GA]AG
             2874  CTCAAG              


#########################################
# 3 OR MORE CUT ENZYMES (  135 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        ApaI        ApoI        
Asp700I     AspHI       AvaI        AvaII       BamHI       
BanI        BanII       BbeI        BbsI        BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BpuAI       BsaHI       BsaJI       BseRI       BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    Bsp1407I    BspLU11I    BspMI       
BsrFI       BsrGI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraI        
DraII       DsaI        EaeI        EagI        EarI        
EclXI       Eco52I      EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
KasI        Ksp632I     KspI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NarI        NciI        
NdeI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PflMI       PleI        PvuII       RsaI        
SacI        SacII       Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SfoI        SmaI        SspBI       
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XhoII       XmaCI       XmaI        XmnI