##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Aor51HI AscI Asp718I AspEI
AspI AvrII BbrPI BbvCI BlnI
BsaAI BsaWI BseAI BsgI BsiEI
BsiWI BspDI BspEI BsrBI BssHII
BstAPI ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
FseI KpnI MluI MroI NcoI
NotI NruI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI PvuII RsrII SalI
SbfI SfiI SnaBI SpeI Sse8387I
StuI Tth111I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AflII 597 CTTAAG C^TTAAG
Alw44I 4753 GTGCAC G^TGCAC
AocI 2303 CCTCAGG CC^T[ACGT]AGG
ApaI 74 GGGCCC GGGCC^C
ApaLI 4753 GTGCAC G^TGCAC
AvaI 207 CCCGGG C^[CT]CG[GA]G
AviII 4690 TGCGCA TGC^GCA
BamHI 2546 GGATCC G^GATCC
BbeI 203 GGCGCC GGCGC^C
BclI 1901 TGATCA T^GATCA
BfrI 597 CTTAAG C^TTAAG
BsaI 3774 GAGACC GGTCTC(1/5)
BsoBI 207 CCCGGG C^[CT]CG[GA]G
BspHI 514 TCATGA T^CATGA
Bst1107I 2947 GTATAC GTA^TAC
BstBI 4474 TTCGAA TT^CGAA
BstEII 256 GGTAACC G^GT[ACGT]ACC
BstPI 256 GGTAACC G^GT[ACGT]ACC
BstZ17I 2947 GTATAC GTA^TAC
Bsu36I 2303 CCTCAGG CC^T[ACGT]AGG
DraIII 639 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2303 CCTCAGG CC^T[ACGT]AGG
EcoNI 3218 CCTTGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 256 GGTAACC G^GT[ACGT]ACC
EcoRI 498 GAATTC G^AATTC
FbaI 1901 TGATCA T^GATCA
FspI 4690 TGCGCA TGC^GCA
HaeII 203 GGCGCC [GA]GCGC^[CT]
KasI 203 GGCGCC G^GCGCC
NarI 203 GGCGCC GG^CGCC
NheI 1644 GCTAGC G^CTAGC
NspV 4474 TTCGAA TT^CGAA
PflMI 641 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 4951 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 514 TCATGA T^CATGA
SacI 4639 GAGCTC GAGCT^C
SapI 4434 GAAGAGC GCTCTTC(1/4)
ScaI 1365 AGTACT AGT^ACT
SexAI 640 ACCAGGT A^CC[AT]GGT
SfoI 203 GGCGCC GGC^GCC
SfuI 4474 TTCGAA TT^CGAA
SmaI 207 CCCGGG CCC^GGG
Van91I 641 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 207 CCCGGG C^CCGGG
XmaI 207 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 203 GGCGCC G[GA]^CG[CT]C
212 GACGCC
BalI 976 TGGCCA TGG^CCA
1111 TGGCCA
BcgI 131 CGAGGGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4466 GCAAAATGTTCG
BglI 241 GCCGAATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5205 GCCAAAAAGGC
BglII 2414 AGATCT A^GATCT
6363 AGATCT
BpmI 2587 CTCCAG CTGGAG(16/14)
984 CTGGAG
BsaHI 203 GGCGCC G[GA]^CG[CT]C
212 GACGCC
BsmBI 2752 CGTCTC CGTCTC(1/5)
1212 GAGACG
BspMI 767 ACCTGC ACCTGC(4/8)
5120 ACCTGC
BssSI 3376 CACGAG CACGAG(-5/-1)
3680 CACGAG
CfoI 204 GCGC GCG^C
4691 GCGC
DraII 74 GGGCCCC [GA]G^G[ACGT]CC[CT]
134 GGGGCCT
EcoO109I 74 GGGCCCC [GA]G^G[ACGT]CC[CT]
134 GGGGCCT
EcoT22I 668 ATGCAT ATGCA^T
1729 ATGCAT
FauI 196 GCGGG CCCGC(4/6)
200 GCGGG
HgaI 212 GACGC GACGC(5/10)
145 GCGTC
HhaI 204 GCGC GCG^C
4691 GCGC
Hin1I 203 GGCGCC G[GA]^CG[CT]C
212 GACGCC
HinP1I 204 GCGC G^CGC
4691 GCGC
HincII 537 GTTAAC GT[CT]^[GA]AC
1277 GTTAAC
HindII 537 GTTAAC GT[CT]^[GA]AC
1277 GTTAAC
HpaI 537 GTTAAC GTT^AAC
1277 GTTAAC
MaeII 462 ACGT A^CGT
2610 ACGT
MfeI 3904 CAATTG C^AATTG
4914 CAATTG
MluNI 976 TGGCCA TGG^CCA
1111 TGGCCA
MscI 976 TGGCCA TGG^CCA
1111 TGGCCA
MunI 3904 CAATTG C^AATTG
4914 CAATTG
NaeI 215 GCCGGC GCC^GGC
324 GCCGGC
NdeI 4717 CATATG CA^TATG
4863 CATATG
NgoMIV 215 GCCGGC G^CCGGC
324 GCCGGC
NsiI 668 ATGCAT ATGCA^T
1729 ATGCAT
SgrAI 214 CGCCGGCG C[GA]^CCGG[CT]G
323 CGCCGGCG
SphI 602 GCATGC GCATG^C
5775 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
AseI AsnI Asp700I AspHI AvaII
BanI BanII BbsI BbvI BciVI
BcnI BfaI BlpI BmyI Bpu1102I
BpuAI BsaBI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspLU11I BsrDI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
Cfr10I Cfr13I DdeI DpnII DraI
DsaI EaeI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HindIII
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PshBI PstI RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI SwaI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII
XmnI