##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AviII AvrII BanI BbeI BbsI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspLU11I
Bst1107I BstBI BstZ17I CelII ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV EcoT14I
FseI FspI HgaI Hin1I HpaI
KasI KpnI KspI MamI MluI
MroI NaeI NarI NcoI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PmeI PshAI Psp1406I PvuI
PvuII RsrII SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I StuI
StyI SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AseI 2627 ATTAAT AT^TAAT
AsnI 2627 ATTAAT AT^TAAT
AspI 2121 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1174 CTCGGG C^[CT]CG[GA]G
BalI 1941 TGGCCA TGG^CCA
BbrPI 234 CACGTG CAC^GTG
BcgI 2036 GCACGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 241 CCCGG CC^[GC]GG
BsaAI 234 CACGTG [CT]AC^GT[GA]
BseRI 57 GAGGAG GAGGAG(10/8)
BsoBI 1174 CTCGGG C^[CT]CG[GA]G
BsrDI 1259 CATTGC GCAATG(2/0)
BssHII 67 GCGCGC G^CGCGC
BssSI 2037 CACGAG CACGAG(-5/-1)
BstEII 623 GGTAACC G^GT[ACGT]ACC
BstPI 623 GGTAACC G^GT[ACGT]ACC
BstXI 1881 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 919 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 75 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1840 CCTGCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 919 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 623 GGTAACC G^GT[ACGT]ACC
FauI 140 CCCGC CCCGC(4/6)
HincII 255 GTTGAC GT[CT]^[GA]AC
HindII 255 GTTGAC GT[CT]^[GA]AC
MluNI 1941 TGGCCA TGG^CCA
MscI 1941 TGGCCA TGG^CCA
MspA1I 51 CCGCTG C[AC]G^C[GT]G
NciI 241 CCCGG CC^[GC]GG
NspI 1819 ACATGC [GA]CATG^[CT]
PflFI 2121 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 234 CACGTG CAC^GTG
PmlI 234 CACGTG CAC^GTG
PpuMI 919 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 2627 ATTAAT AT^TAAT
SexAI 2949 ACCAGGT A^CC[AT]GGT
TaqI 542 TCGA T^CGA
Tth111I 2121 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 542 TCGA T^CGA
XbaI 1520 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccI 945 GTAGAC GT^[AC][GT]AC
3297 GTCTAC
AgeI 227 ACCGGT A^CCGGT
1327 ACCGGT
AlwNI 630 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
3207 CAGCCTCTG
AocI 248 CCTCAGG CC^T[ACGT]AGG
1562 CCTAAGG
Asp700I 225 GAACCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1509 GAATGATTTC
BamHI 966 GGATCC G^GATCC
1178 GGATCC
BbvCI 2030 GCTGAGG CCTCAGC(-5/-2)
2497 GCTGAGG
BciVI 2615 GGATAC GTATCC(6/5)
192 GTATCC
BclI 2547 TGATCA T^GATCA
2855 TGATCA
BglI 1943 GCCAATATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2053 GCCTGGGAGGC
BglII 862 AGATCT A^GATCT
2082 AGATCT
BsaWI 227 ACCGGT [AT]^CCGG[AT]
1327 ACCGGT
BsgI 2093 CTGCAC GTGCAG(16/14)
2723 CTGCAC
BsmI 1052 GAATGC GAATGC(1/-1)
1700 GAATGC
Bsp1407I 3075 TGTACA T^GTACA
3109 TGTACA
BspMI 259 ACCTGC ACCTGC(4/8)
2155 ACCTGC
BsrBI 96 CCGCTC CCGCTC(-3/-3)
141 CCGCTC
BsrFI 227 ACCGGT [GA]^CCGG[CT]
1327 ACCGGT
BsrGI 3075 TGTACA T^GTACA
3109 TGTACA
BstAPI 86 GCAGGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2062 GCAGAGGTTGC
Bsu36I 248 CCTCAGG CC^T[ACGT]AGG
1562 CCTAAGG
Cfr10I 227 ACCGGT [GA]^CCGG[CT]
1327 ACCGGT
DraI 596 TTTAAA TTT^AAA
2822 TTTAAA
DsaI 1196 CCGTGG C^C[GA][CT]GG
2777 CCGTGG
EaeI 1255 CGGCCA [CT]^GGCC[GA]
1941 TGGCCA
Eco81I 248 CCTCAGG CC^T[ACGT]AGG
1562 CCTAAGG
FbaI 2547 TGATCA T^GATCA
2855 TGATCA
HaeII 17 GGCGCT [GA]GCGC^[CT]
47 AGCGCC
HindIII 1348 AAGCTT A^AGCTT
1719 AAGCTT
MfeI 1402 CAATTG C^AATTG
3157 CAATTG
MunI 1402 CAATTG C^AATTG
3157 CAATTG
NdeI 2600 CATATG CA^TATG
3094 CATATG
PinAI 227 ACCGGT A^CCGGT
1327 ACCGGT
PstI 84 CTGCAG CTGCA^G
261 CTGCAG
SapI 143 GCTCTTC GCTCTTC(1/4)
1190 GCTCTTC
SpeI 1373 ACTAGT A^CTAGT
2908 ACTAGT
SspBI 3075 TGTACA T^GTACA
3109 TGTACA
SspI 2277 AATATT AAT^ATT
2972 AATATT
XmnI 225 GAACCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1509 GAATGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BanII
BbvI BfaI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoRII
EcoT22I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV NsiI PflMI
PleI RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XcmI XhoII