##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AviII       AvrII       BbrPI       BcgI        BciVI       
BglI        BglII       BlnI        BsaAI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BspEI       BsrBI       BsrDI       BssHII      BssSI       
BstXI       CpoI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoRV       FseI        FspI        HgaI        HindIII     
KpnI        KspI        MaeII       MfeI        MluI        
MroI        MunI        NcoI        NdeI        NotI        
NruI        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RsrII       
SacI        SacII       SalI        SbfI        SfiI        
SgrAI       SnaBI       SphI        Sse8387I    SwaI        
XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   70 enzymes) #
###################################

AccI         3314  GTATAC               GT^[AC][GT]AC
AccII        2643  CGCG                 CG^CG
AcyI           17  GGCGCC               G[GA]^CG[CT]C
AflII        3256  CTTAAG               C^TTAAG
AflIII        726  ACATGT               A^C[GA][CT]GT
Asp700I       518  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1163  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          112  CCCGGG               C^[CT]CG[GA]G
BalI          225  TGGCCA               TGG^CCA
BamHI        1027  GGATCC               G^GATCC
BbeI           17  GGCGCC               GGCGC^C
BbvCI        1655  CCTCAGC              CCTCAGC(-5/-2)
BfrI         3256  CTTAAG               C^TTAAG
BlpI         1313  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1313  GCTCAGC              GC^T[ACGT]AGC
BsaHI          17  GGCGCC               G[GA]^CG[CT]C
BsmAI        1277  GAGAC                GTCTC(1/5)
BsmFI        2145  GGGAC                GGGAC(10/14)
BsmI          425  GCATTC               GAATGC(1/-1)
BsoBI         112  CCCGGG               C^[CT]CG[GA]G
Bsp1407I     2183  TGTACA               T^GTACA
BspLU11I      726  ACATGT               A^CATGT
BsrFI          20  GCCGGC               [GA]^CCGG[CT]
BsrGI        2183  TGTACA               T^GTACA
Bst1107I     3314  GTATAC               GTA^TAC
BstAPI       2981  GCAGACGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         375  TTCGAA               TT^CGAA
BstEII       2589  GGTCACC              G^GT[ACGT]ACC
BstPI        2589  GGTCACC              G^GT[ACGT]ACC
BstUI        2643  CGCG                 CG^CG
BstZ17I      3314  GTATAC               GTA^TAC
CelII        1313  GCTCAGC              GC^T[ACGT]AGC
CfoI           18  GCGC                 GCG^C
Cfr10I         20  GCCGGC               [GA]^CCGG[CT]
DraII         217  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoO109I      217  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I      2589  GGTCACC              G^GT[ACGT]ACC
EcoRI        1427  GAATTC               G^AATTC
EcoT22I      1812  ATGCAT               ATGCA^T
HaeII          17  GGCGCC               [GA]GCGC^[CT]
HhaI           18  GCGC                 GCG^C
Hin1I          17  GGCGCC               G[GA]^CG[CT]C
HinP1I         18  GCGC                 G^CGC
KasI           17  GGCGCC               G^GCGCC
MluNI         225  TGGCCA               TGG^CCA
MscI          225  TGGCCA               TGG^CCA
MslI          562  CATCGATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        139  CAGCGG               C[AC]G^C[GT]G
MvnI         2643  CGCG                 CG^CG
NaeI           20  GCCGGC               GCC^GGC
NarI           17  GGCGCC               GG^CGCC
NgoMIV         20  GCCGGC               G^CCGGC
NheI          238  GCTAGC               G^CTAGC
NsiI         1812  ATGCAT               ATGCA^T
NspV          375  TTCGAA               TT^CGAA
PflFI        1163  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         228  CCAAAATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI         1520  GAAGAGC              GCTCTTC(1/4)
ScaI         3033  AGTACT               AGT^ACT
SfoI           17  GGCGCC               GGC^GCC
SfuI          375  TTCGAA               TT^CGAA
SmaI          112  CCCGGG               CCC^GGG
SpeI         2114  ACTAGT               A^CTAGT
SspBI        2183  TGTACA               T^GTACA
StuI         2803  AGGCCT               AGG^CCT
Tth111I      1163  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        228  CCAAAATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         112  CCCGGG               C^CCGGG
XmaI          112  CCCGGG               C^CCGGG
XmnI          518  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AlwNI         539  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1638  CAGAGCCTG           
AspHI         775  GAGCAC               G[AT]GC[AT]^C
             1890  GTGCTC              
AvaII        1468  GGACC                G^G[AT]CC
             2057  GGACC               
BanI           17  GGCGCC               G^G[CT][GA]CC
             1243  GGCACC              
BbsI         1379  GAAGAC               GAAGAC(2/6)
             1881  GTCTTC              
BclI          535  TGATCA               T^GATCA
             1057  TGATCA              
BcnI          112  CCCGG                CC^[GC]GG
              113  CCGGG               
BpmI         1535  CTCCAG               CTGGAG(16/14)
             1905  CTGGAG              
BpuAI        1379  GAAGAC               GAAGAC(2/6)
             1881  GTCTTC              
BsaBI        1826  GATCAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2396  GATTTTTATC          
BsgI          960  CTGCAC               GTGCAG(16/14)
             3056  CTGCAC              
BsiHKAI       775  GAGCAC               G[AT]GC[AT]^C
             1890  GTGCTC              
BspDI         563  ATCGAT               AT^CGAT
             1502  ATCGAT              
BstYI        1027  GGATCC               [GA]^GATC[CT]
             1237  GGATCT              
ClaI          563  ATCGAT               AT^CGAT
             1502  ATCGAT              
EaeI          225  TGGCCA               [CT]^GGCC[GA]
              452  CGGCCA              
EcoNI         469  CCTGCCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              925  CCTCTCTGAGG         
FauI          126  CCCGC                CCCGC(4/6)
               36  GCGGG               
FbaI          535  TGATCA               T^GATCA
             1057  TGATCA              
HapII          21  CCGG                 C^CGG
              113  CCGG                
HpaI         2549  GTTAAC               GTT^AAC
             3236  GTTAAC              
HpaII          21  CCGG                 C^CGG
              113  CCGG                
MamI         1826  GATCAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2396  GATTTTTATC          
MflI         1027  GGATCC               [GA]^GATC[CT]
             1237  GGATCT              
MspI           21  CCGG                 C^CGG
              113  CCGG                
NciI          112  CCCGG                CC^[GC]GG
              113  CCGGG               
NspI          726  ACATGT               [GA]CATG^[CT]
             2361  GCATGT              
SexAI        2694  ACCTGGT              A^CC[AT]GGT
             2959  ACCTGGT             
SfcI         1686  CTGTAG               C^T[GA][CT]AG
             1975  CTACAG              
XhoII        1027  GGATCC               [GA]^GATC[CT]
             1237  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AocI        ApoI        BanII       BbvI        BfaI        
BmyI        BsaJI       BseRI       BsiYI       BslI        
Bsp1286I    BspHI       BspMI       BsrI        BssKI       
BstF5I      BstNI       Bsu36I      Cac8I       Cfr13I      
DdeI        DpnII       DraI        EarI        Eco81I      
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HincII      HindII      HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MnlI        MseI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        RcaI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SmlI        
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I