##################################
# NO CUT ENZYMES (   83 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AgeI        
Alw44I      ApaLI       AscI        Asp718I     AspI        
AviII       AvrII       BbeI        BbrPI       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaWI       
BseAI       BsiEI       BsiWI       BspDI       BspEI       
BspHI       BssHII      BssSI       BstBI       BstEII      
BstPI       BstUI       CelII       ClaI        CpoI        
DraIII      DrdI        EagI        EclXI       Eco52I      
EcoO65I     EcoT22I     FauI        FspI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MunI        MvnI        NarI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PvuI        RcaI        RsrII       
SacII       SalI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        SwaI        Tth111I     
Van91I      XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccI         1502  GTATAC               GT^[AC][GT]AC
AclI         4465  AACGTT               AA^CGTT
AcyI         1516  GGCGTC               G[GA]^CG[CT]C
AflIII       3916  ACATGT               A^C[GA][CT]GT
AhdI         1815  GACCTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI           32  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       338  AGCGCT               AGC^GCT
ApaI         4572  GGGCCC               GGGCC^C
AseI         3946  ATTAAT               AT^TAAT
AsnI         3946  ATTAAT               AT^TAAT
AspEI        1815  GACCTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI          884  TGGCCA               TGG^CCA
BamHI         527  GGATCC               G^GATCC
BcgI          534  GCAGTGCAGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        3880  AGATCT               A^GATCT
BsaHI        1516  GGCGTC               G[GA]^CG[CT]C
BsmBI         615  GAGACG               CGTCTC(1/5)
BspLU11I     3916  ACATGT               A^CATGT
BsrBI        4498  GAGCGG               CCGCTC(-3/-3)
BsrFI        1446  GCCGGC               [GA]^CCGG[CT]
Bst1107I     1502  GTATAC               GTA^TAC
BstXI        1535  CCAGCAATTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1502  GTATAC               GTA^TAC
Bsu36I         32  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       1446  GCCGGC               [GA]^CCGG[CT]
Eam1105I     1815  GACCTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      338  AGCGCT               AGC^GCT
Eco81I         32  CCTGAGG              CC^T[ACGT]AGG
EcoRI        1136  GAATTC               G^AATTC
FseI         1445  GGCCGGCC             GGCCGG^CC
HaeII         338  AGCGCT               [GA]GCGC^[CT]
HgaI         1517  GCGTC                GACGC(5/10)
Hin1I        1516  GGCGTC               G[GA]^CG[CT]C
HincII        636  GTTAAC               GT[CT]^[GA]AC
HindII        636  GTTAAC               GT[CT]^[GA]AC
HpaI          636  GTTAAC               GTT^AAC
MluNI         884  TGGCCA               TGG^CCA
MscI          884  TGGCCA               TGG^CCA
NaeI         1446  GCCGGC               GCC^GGC
NdeI          880  CATATG               CA^TATG
NgoMIV       1446  GCCGGC               G^CCGGC
PshAI        2149  GACAGAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        3946  ATTAAT               AT^TAAT
Psp1406I     4465  AACGTT               AA^CGTT
SapI         2741  GAAGAGC              GCTCTTC(1/4)
SbfI         2978  CCTGCAGG             CCTGCA^GG
ScaI          961  AGTACT               AGT^ACT
SexAI        4326  ACCTGGT              A^CC[AT]GGT
SfiI         4564  GGCCAGGAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         4575  CCCGGG               CCC^GGG
Sse8387I     2978  CCTGCAGG             CCTGCA^GG
XmaCI        4575  CCCGGG               C^CCGGG
XmaI         4575  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

Asp700I      1665  GAAACTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2994  GAACTTCTTC          
AvaI         1549  CTCGGG               C^[CT]CG[GA]G
             4575  CCCGGG              
BbvCI        2226  GCTGAGG              CCTCAGC(-5/-2)
             2469  GCTGAGG             
BciVI         306  GTATCC               GTATCC(6/5)
             3646  GTATCC              
BclI         1621  TGATCA               T^GATCA
             2687  TGATCA              
BsaI          920  GAGACC               GGTCTC(1/5)
              118  GGTCTC              
BsoBI        1549  CTCGGG               C^[CT]CG[GA]G
             4575  CCCGGG              
BstAPI        790  GCAACAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1046  GCAAAGGTTGC         
CfoI          339  GCGC                 GCG^C
             2400  GCGC                
DsaI         1145  CCATGG               C^C[GA][CT]GG
             3629  CCATGG              
EaeI          884  TGGCCA               [CT]^GGCC[GA]
             1448  CGGCCA              
EcoRV         228  GATATC               GAT^ATC
             3397  GATATC              
FbaI         1621  TGATCA               T^GATCA
             2687  TGATCA              
HhaI          339  GCGC                 GCG^C
             2400  GCGC                
HinP1I        339  GCGC                 G^CGC
             2400  GCGC                
NcoI         1145  CCATGG               C^CATGG
             3629  CCATGG              
NspI         1903  GCATGT               [GA]CATG^[CT]
             3916  ACATGT              
SspI         3788  AATATT               AAT^ATT
             4239  AATATT              
StuI           36  AGGCCT               AGG^CCT
             2414  AGGCCT              
TaqI          232  TCGA                 T^CGA
             4559  TCGA                
TthHB8I       232  TCGA                 T^CGA
             4559  TCGA                
XcmI         2365  CCAGCCGCCCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4615  CCACCTTAAGAATGG     
XmnI         1665  GAAACTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2994  GAACTTCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   99 enzymes) #
#########################################

AciI        AcsI        AfaI        AflII       AluI        
AlwI        AlwNI       ApoI        AspHI       AvaII       
BanI        BanII       BbsI        BbvI        BcnI        
BfaI        BfrI        BglI        BmyI        BpmI        
BpuAI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
Bsp1286I    Bsp1407I    BspMI       BsrDI       BsrGI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DraI        
DraII       EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspBI       StyI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       XhoII