##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
Acc65I AccI AclI AgeI AscI
AseI AsnI Asp718I AspI AviII
AvrII BbeI BciVI BclI BlnI
BsiEI BsiWI Bsp1407I BspDI BspHI
BsrGI BssSI Bst1107I BstBI BstZ17I
ClaI CpoI EagI EclXI Eco52I
EcoRV FbaI FseI FspI HindIII
HpaI KasI KpnI MfeI MluI
MunI NarI NdeI NheI NotI
NruI NspV PaeR7I PflFI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SspBI
SspI SwaI TaqI Tth111I TthHB8I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 1196 GACGTC GACGT^C
AccIII 591 TCCGGA T^CCGGA
AcyI 1196 GACGTC G[GA]^CG[CT]C
AhdI 1749 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4053 AGCGCT AGC^GCT
Asp700I 3264 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1749 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2713 GGATCC G^GATCC
BcgI 2443 GCAGTGGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 3714 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1196 GACGTC G[GA]^CG[CT]C
BseAI 591 TCCGGA T^CCGGA
BspEI 591 TCCGGA T^CCGGA
BspLU11I 2134 ACATGT A^CATGT
BssHII 1007 GCGCGC G^CGCGC
BstEII 2665 GGTGACC G^GT[ACGT]ACC
BstPI 2665 GGTGACC G^GT[ACGT]ACC
DraI 2304 TTTAAA TTT^AAA
DrdI 3486 GACCATGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1749 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4053 AGCGCT AGC^GCT
EcoO65I 2665 GGTGACC G^GT[ACGT]ACC
EcoRI 386 GAATTC G^AATTC
EcoT22I 3499 ATGCAT ATGCA^T
Hin1I 1196 GACGTC G[GA]^CG[CT]C
HincII 1058 GTCAAC GT[CT]^[GA]AC
HindII 1058 GTCAAC GT[CT]^[GA]AC
MamI 3714 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 591 TCCGGA T^CCGGA
NsiI 3499 ATGCAT ATGCA^T
PacI 2808 TTAATTAA TTAAT^TAA
PshAI 4167 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 4537 CCTGCAGG CCTGCA^GG
SphI 3440 GCATGC GCATG^C
Sse8387I 4537 CCTGCAGG CCTGCA^GG
XmnI 3264 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AflII 2288 CTTAAG C^TTAAG
3507 CTTAAG
BbvCI 24 GCTGAGG CCTCAGC(-5/-2)
152 GCTGAGG
BfrI 2288 CTTAAG C^TTAAG
3507 CTTAAG
BglII 2519 AGATCT A^GATCT
3849 AGATCT
BsaWI 591 TCCGGA [AT]^CCGG[AT]
4927 TCCGGT
HaeII 1144 GGCGCT [GA]GCGC^[CT]
4053 AGCGCT
HgaI 2129 GACGC GACGC(5/10)
3647 GACGC
KspI 32 CCGCGG CCGC^GG
2921 CCGCGG
NaeI 515 GCCGGC GCC^GGC
4332 GCCGGC
NgoMIV 515 GCCGGC G^CCGGC
4332 GCCGGC
SacII 32 CCGCGG CCGC^GG
2921 CCGCGG
SexAI 3806 ACCTGGT A^CC[AT]GGT
4091 ACCAGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI AspHI AvaI
AvaII BalI BanI BanII BbrPI
BbsI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XhoII XmaCI XmaI